LOCUS       BBa_K518004             1728 bp    DNA     linear       14-Nov-2024
DEFINITION  IPTG-inducible L-Asp producing device
ACCESSION   BBa_K518004
VERSION     BBa_K518004.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     CDS             83..1561
                     /label=AspA
     misc_feature    83..83
                     /label=G
     misc_feature    670..670
                     /label=C
     misc_feature    706..706
                     /label=G
     misc_feature    739..739
                     /label=C
     misc_feature    1081..1081
                     /label=G
     misc_feature    1207..1207
                     /label=G
     misc_feature    1562..1594
                     /label=LVA
     misc_feature    1601..1625
                     /label=Help:Barcodes
     CDS             83..1625
                     /label=BBa_C0083
     stem_loop       1641..1660
                     /label=T7 TE
     misc_feature    1667..1667
                     /label=stop
     polyA_site      1661..1674
                     /label=polya
     misc_feature    1634..1674
                     /label=BBa_B0012
     terminator      1634..1674
                     /label=BBa_B0012
     stem_loop       1695..1717
                     /label=stem_loop
     misc_feature    1683..1728
                     /label=BBa_B0011
     terminator      1683..1728
                     /label=BBa_B0011
     terminator      1634..1728
                     /label=BBa_B0014
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagtact agatgtgttt aaagcaaatc attggcagct tgaaaaagaa
      121 ggttcacatg tcaaacaaca ttcgtatcga agaagatctg ttgggtacca gggaagttcc
      181 agctgatgcc tactatggtg ttcacactct gagagcgatt gaaaacttct atatcagcaa
      241 caacaaaatc agtgatattc ctgaatttgt tcgcggtatg gtaatggtta aaaaagccgc
      301 agctatggca aacaaagagc tgcaaaccat tcctaaaagt gtagcgaatg ccatcattgc
      361 cgcatgtgat gaagtcctga acaacggaaa atgcatggat cagttcccgg tagacgtcta
      421 ccagggcggc gcaggtactt ccgtaaacat gaacaccaac gaagtgctgg ccaatatcgg
      481 tctggaactg atgggtcacc aaaaaggtga atatcagtac ctgaacccga acgaccatgt
      541 taacaaatgt cagtccacta acgacgccta cccgaccggt ttccgtatcg cagtttactc
      601 ttccctgatt aagctggtag atgcgattaa ccaactgcgt gaaggctttg aacgtaaagc
      661 tgtcgaattt caggacatcc tgaaaatggg tcgtacccag ctgcaagacg cagtaccgat
      721 gaccctcggt caggaatttc gcgctttcag catcctgctg aaagaagaag tgaaaaacat
      781 ccaacgtacc gctgaactgc tgctggaagt taaccttggt gcaacagcaa tcggtactgg
      841 tctgaacacg ccgaaagagt actctccgct ggcagtgaaa aaactggctg aagttactgg
      901 cttcccatgc gtaccggctg aagacctgat cgaagcgacc tctgactgcg gcgcttatgt
      961 tatggttcac ggcgcgctga aacgcctggc tgtgaagatg tccaaaatct gtaacgacct
     1021 gcgcttgctc tcttcaggcc cacgtgccgg cctgaacgag atcaacctgc cggaactgca
     1081 agcgggctct tccatcatgc cagctaaagt aaacccggtt gttccggaag tggttaacca
     1141 ggtatgcttc aaagtcatcg gtaacgacac cactgttacc atggcagcag aagcaggtca
     1201 gctgcaattg aacgttatgg agccggtcat tggccaggcc atgttcgaat ccgttcacat
     1261 tctgaccaac gcttgctaca acctgctgga aaaatgcatt aacggcatca ctgctaacaa
     1321 agaagtgtgc gaaggttacg tttacaactc tatcggtatc gttacttacc tgaacccgtt
     1381 catcggtcac cacaacggtg acatcgtggg taaaatctgt gccgaaaccg gtaagagtgt
     1441 acgtgaagtc gttctggaac gcggtctgtt gactgaagcg gaacttgacg atattttctc
     1501 cgtacagaat ctgatgcacc cggcttacaa agcaaaacgc tatactgatg aaagcgaaca
     1561 ggctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag
     1621 atctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     1681 agagagaata taaaaagcca gattattaat ccggcttttt tattattt
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