##gff-version 3
##sequence-region BBa_K518004 1 1728
BBa_K518004	.	terminator	1634	1728	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K518004	.	terminator	1683	1728	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K518004	.	stem_loop	1695	1717	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K518004	.	engineered_region	1683	1728	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K518004	.	terminator	1634	1674	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K518004	.	engineered_region	1634	1674	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K518004	.	stop_codon	1667	1667	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K518004	.	stem_loop	1641	1660	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K518004	.	polyA_site	1661	1674	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K518004	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K518004	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K518004	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K518004	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K518004	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K518004	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K518004	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K518004	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K518004	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K518004	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K518004	.	CDS	83	1625	.	+	0	ID=BBa_C0083;Name=BBa_C0083
BBa_K518004	.	point_mutation	83	83	.	+	0	ID=annotation300982;Name=G;Parent=BBa_C0083
BBa_K518004	.	point_mutation	1081	1081	.	+	0	ID=annotation300987;Name=G;Parent=BBa_C0083
BBa_K518004	.	point_mutation	739	739	.	+	0	ID=annotation300986;Name=C;Parent=BBa_C0083
BBa_K518004	.	tag	1562	1594	.	+	0	ID=annotation300993;Name=LVA;Parent=BBa_C0083
BBa_K518004	.	point_mutation	1207	1207	.	+	0	ID=annotation300988;Name=G;Parent=BBa_C0083
BBa_K518004	.	point_mutation	670	670	.	+	0	ID=annotation300983;Name=C;Parent=BBa_C0083
BBa_K518004	.	CDS	83	1561	.	+	0	ID=annotation300981;Name=AspA;Parent=BBa_C0083
BBa_K518004	.	engineered_tag	1601	1625	.	+	0	ID=annotation2213998;Name=Help:Barcodes;Parent=BBa_C0083
BBa_K518004	.	point_mutation	706	706	.	+	0	ID=annotation300984;Name=G;Parent=BBa_C0083
>BBa_K518004
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgtgtttaaagcaaatcattggcagcttgaaaaagaaggttcacatgtcaaacaacattcgtatcgaagaagatc
tgttgggtaccagggaagttccagctgatgcctactatggtgttcacactctgagagcgattgaaaacttctatatcag
caacaacaaaatcagtgatattcctgaatttgttcgcggtatggtaatggttaaaaaagccgcagctatggcaaacaaa
gagctgcaaaccattcctaaaagtgtagcgaatgccatcattgccgcatgtgatgaagtcctgaacaacggaaaatgca
tggatcagttcccggtagacgtctaccagggcggcgcaggtacttccgtaaacatgaacaccaacgaagtgctggccaa
tatcggtctggaactgatgggtcaccaaaaaggtgaatatcagtacctgaacccgaacgaccatgttaacaaatgtcag
tccactaacgacgcctacccgaccggtttccgtatcgcagtttactcttccctgattaagctggtagatgcgattaacc
aactgcgtgaaggctttgaacgtaaagctgtcgaatttcaggacatcctgaaaatgggtcgtacccagctgcaagacgc
agtaccgatgaccctcggtcaggaatttcgcgctttcagcatcctgctgaaagaagaagtgaaaaacatccaacgtacc
gctgaactgctgctggaagttaaccttggtgcaacagcaatcggtactggtctgaacacgccgaaagagtactctccgc
tggcagtgaaaaaactggctgaagttactggcttcccatgcgtaccggctgaagacctgatcgaagcgacctctgactg
cggcgcttatgttatggttcacggcgcgctgaaacgcctggctgtgaagatgtccaaaatctgtaacgacctgcgcttg
ctctcttcaggcccacgtgccggcctgaacgagatcaacctgccggaactgcaagcgggctcttccatcatgccagcta
aagtaaacccggttgttccggaagtggttaaccaggtatgcttcaaagtcatcggtaacgacaccactgttaccatggc
agcagaagcaggtcagctgcaattgaacgttatggagccggtcattggccaggccatgttcgaatccgttcacattctg
accaacgcttgctacaacctgctggaaaaatgcattaacggcatcactgctaacaaagaagtgtgcgaaggttacgttt
acaactctatcggtatcgttacttacctgaacccgttcatcggtcaccacaacggtgacatcgtgggtaaaatctgtgc
cgaaaccggtaagagtgtacgtgaagtcgttctggaacgcggtctgttgactgaagcggaacttgacgatattttctcc
gtacagaatctgatgcacccggcttacaaagcaaaacgctatactgatgaaagcgaacaggctgcaaacgacgaaaact
acgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt