LOCUS       BBa_K518006              992 bp    DNA     linear       22-Dec-2024
DEFINITION  IPTG-inducible CheZ expression device
ACCESSION   BBa_K518006
VERSION     BBa_K518006.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     CDS             85..889
                     /label=BBa_K518005
     stem_loop       905..924
                     /label=T7 TE
     misc_feature    931..931
                     /label=stop
     polyA_site      925..938
                     /label=polya
     misc_feature    898..938
                     /label=BBa_B0012
     terminator      898..938
                     /label=BBa_B0012
     stem_loop       959..981
                     /label=stem_loop
     misc_feature    947..992
                     /label=BBa_B0011
     terminator      947..992
                     /label=BBa_B0011
     terminator      898..992
                     /label=BBa_B0014
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagtact agagatcatt gctgcggcgc aagcgggggc cagtggctat
      121 gtggtgaagc catttaccgc cgcgacgctg gaggaaaaac tcaacaaaat ctttgagaaa
      181 ctgggcatgt gaggatgcga ctatgatgca accatcaatc aaacctgctg acgagcattc
      241 agctggcgat atcattgcgc gcatcggcag cctgacgcgt atgctgcgcg acagtttgcg
      301 ggaactgggg ctggatcagg ccattgccga agcggcggaa gccatccccg atgcgcgcga
      361 tcgtttgtac tatgttgtgc agatgaccgc ccaggctgcg gagcgggcgc tgaacagtgt
      421 tgaggcgtca caaccgcatc aggatcaaat ggagaaatca gcaaaagcgt taacccaacg
      481 ttgggatgac tggtttgccg atccgattga ccttgccgac gcccgtgaac tggtaacaga
      541 tacacgacaa tttctggcag atgtacccgc gcataccagc tttactaacg cgcaactgct
      601 ggaaatcatg atggcgcagg attttcagga tctcaccggg caggtcatta agcggatgat
      661 ggatgtcatt caggagatcg aacgccagtt gctgatggtg ctgttggaaa acatcccgga
      721 acaggagtcg cgtccaaaac gtgaaaacca gagtttgctt aatggacctc aggtcgatac
      781 cagcaaagcc ggtgtggtag ccagtcagga tcaggtggac gatttgttgg atagtcttgg
      841 attttgattt gtattgcctg atgtggcgtg accacgtcat atcaggcggt actagagtca
      901 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
      961 gccagattat taatccggct tttttattat tt
//