LOCUS BBa_K518007 929 bp DNA linear 24-Nov-2024 DEFINITION cheZ expression cassette (no promoter) ACCESSION BBa_K518007 VERSION BBa_K518007.1 FEATURES Location/Qualifiers RBS 1..13 /label=RBS-3\Weak RBS 7..10 /label=RBS misc_feature 1..13 /label=BBa_B0032 RBS 1..13 /label=BBa_B0032 CDS 22..826 /label=BBa_K518005 stem_loop 842..861 /label=T7 TE misc_feature 868..868 /label=stop polyA_site 862..875 /label=polya misc_feature 835..875 /label=BBa_B0012 terminator 835..875 /label=BBa_B0012 stem_loop 896..918 /label=stem_loop misc_feature 884..929 /label=BBa_B0011 terminator 884..929 /label=BBa_B0011 terminator 835..929 /label=BBa_B0014 ORIGIN 1 tcacacagga aagtactaga gatcattgct gcggcgcaag cgggggccag tggctatgtg 61 gtgaagccat ttaccgccgc gacgctggag gaaaaactca acaaaatctt tgagaaactg 121 ggcatgtgag gatgcgacta tgatgcaacc atcaatcaaa cctgctgacg agcattcagc 181 tggcgatatc attgcgcgca tcggcagcct gacgcgtatg ctgcgcgaca gtttgcggga 241 actggggctg gatcaggcca ttgccgaagc ggcggaagcc atccccgatg cgcgcgatcg 301 tttgtactat gttgtgcaga tgaccgccca ggctgcggag cgggcgctga acagtgttga 361 ggcgtcacaa ccgcatcagg atcaaatgga gaaatcagca aaagcgttaa cccaacgttg 421 ggatgactgg tttgccgatc cgattgacct tgccgacgcc cgtgaactgg taacagatac 481 acgacaattt ctggcagatg tacccgcgca taccagcttt actaacgcgc aactgctgga 541 aatcatgatg gcgcaggatt ttcaggatct caccgggcag gtcattaagc ggatgatgga 601 tgtcattcag gagatcgaac gccagttgct gatggtgctg ttggaaaaca tcccggaaca 661 ggagtcgcgt ccaaaacgtg aaaaccagag tttgcttaat ggacctcagg tcgataccag 721 caaagccggt gtggtagcca gtcaggatca ggtggacgat ttgttggata gtcttggatt 781 ttgatttgta ttgcctgatg tggcgtgacc acgtcatatc aggcggtact agagtcacac 841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc 901 agattattaa tccggctttt ttattattt //