LOCUS       BBa_K518007              929 bp    DNA     linear       25-Dec-2024
DEFINITION  cheZ expression cassette (no promoter)
ACCESSION   BBa_K518007
VERSION     BBa_K518007.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             22..826
                     /label=BBa_K518005
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     stem_loop       896..918
                     /label=stem_loop
     misc_feature    884..929
                     /label=BBa_B0011
     terminator      884..929
                     /label=BBa_B0011
     terminator      835..929
                     /label=BBa_B0014
ORIGIN
        1 tcacacagga aagtactaga gatcattgct gcggcgcaag cgggggccag tggctatgtg
       61 gtgaagccat ttaccgccgc gacgctggag gaaaaactca acaaaatctt tgagaaactg
      121 ggcatgtgag gatgcgacta tgatgcaacc atcaatcaaa cctgctgacg agcattcagc
      181 tggcgatatc attgcgcgca tcggcagcct gacgcgtatg ctgcgcgaca gtttgcggga
      241 actggggctg gatcaggcca ttgccgaagc ggcggaagcc atccccgatg cgcgcgatcg
      301 tttgtactat gttgtgcaga tgaccgccca ggctgcggag cgggcgctga acagtgttga
      361 ggcgtcacaa ccgcatcagg atcaaatgga gaaatcagca aaagcgttaa cccaacgttg
      421 ggatgactgg tttgccgatc cgattgacct tgccgacgcc cgtgaactgg taacagatac
      481 acgacaattt ctggcagatg tacccgcgca taccagcttt actaacgcgc aactgctgga
      541 aatcatgatg gcgcaggatt ttcaggatct caccgggcag gtcattaagc ggatgatgga
      601 tgtcattcag gagatcgaac gccagttgct gatggtgctg ttggaaaaca tcccggaaca
      661 ggagtcgcgt ccaaaacgtg aaaaccagag tttgcttaat ggacctcagg tcgataccag
      721 caaagccggt gtggtagcca gtcaggatca ggtggacgat ttgttggata gtcttggatt
      781 ttgatttgta ttgcctgatg tggcgtgacc acgtcatatc aggcggtact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc
      901 agattattaa tccggctttt ttattattt
//