LOCUS       BBa_K518008             1954 bp    DNA     linear       25-Nov-2024
DEFINITION  IPTG-inducible CheZ  repression device
ACCESSION   BBa_K518008
VERSION     BBa_K518008.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..70
                     /label=RBS-3\Weak
     RBS             64..67
                     /label=RBS
     misc_feature    58..70
                     /label=BBa_B0032
     RBS             58..70
                     /label=BBa_B0032
     CDS             79..883
                     /label=BBa_K518005
     stem_loop       899..918
                     /label=T7 TE
     misc_feature    925..925
                     /label=stop
     polyA_site      919..932
                     /label=polya
     misc_feature    892..932
                     /label=BBa_B0012
     terminator      892..932
                     /label=BBa_B0012
     stem_loop       953..975
                     /label=stem_loop
     misc_feature    941..986
                     /label=BBa_B0011
     terminator      941..986
                     /label=BBa_B0011
     terminator      892..986
                     /label=BBa_B0014
     misc_feature    997..1013
                     /label=lac O1
     promoter        1014..1019
                     /label=-35
     misc_feature    1020..1036
                     /label=lac O1
     promoter        1037..1042
                     /label=-10
     misc_feature    995..1048
                     /label=BBa_R0011
     promoter        995..1048
                     /label=BBa_R0011
     RBS             1058..1070
                     /label=RBS-3\Weak
     RBS             1064..1067
                     /label=RBS
     misc_feature    1058..1070
                     /label=BBa_B0032
     RBS             1058..1070
                     /label=BBa_B0032
     CDS             1080..1787
                     /label=cI lambda
     misc_feature    1788..1820
                     /label=LVA
     misc_feature    1827..1851
                     /label=Help:Barcodes
     CDS             1077..1851
                     /label=BBa_C0051
     stem_loop       1867..1886
                     /label=T7 TE
     misc_feature    1893..1893
                     /label=stop
     polyA_site      1887..1900
                     /label=polya
     misc_feature    1860..1900
                     /label=BBa_B0012
     terminator      1860..1900
                     /label=BBa_B0012
     stem_loop       1921..1943
                     /label=stem_loop
     misc_feature    1909..1954
                     /label=BBa_B0011
     terminator      1909..1954
                     /label=BBa_B0011
     terminator      1860..1954
                     /label=BBa_B0014
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca
       61 cacaggaaag tactagagat cattgctgcg gcgcaagcgg gggccagtgg ctatgtggtg
      121 aagccattta ccgccgcgac gctggaggaa aaactcaaca aaatctttga gaaactgggc
      181 atgtgaggat gcgactatga tgcaaccatc aatcaaacct gctgacgagc attcagctgg
      241 cgatatcatt gcgcgcatcg gcagcctgac gcgtatgctg cgcgacagtt tgcgggaact
      301 ggggctggat caggccattg ccgaagcggc ggaagccatc cccgatgcgc gcgatcgttt
      361 gtactatgtt gtgcagatga ccgcccaggc tgcggagcgg gcgctgaaca gtgttgaggc
      421 gtcacaaccg catcaggatc aaatggagaa atcagcaaaa gcgttaaccc aacgttggga
      481 tgactggttt gccgatccga ttgaccttgc cgacgcccgt gaactggtaa cagatacacg
      541 acaatttctg gcagatgtac ccgcgcatac cagctttact aacgcgcaac tgctggaaat
      601 catgatggcg caggattttc aggatctcac cgggcaggtc attaagcgga tgatggatgt
      661 cattcaggag atcgaacgcc agttgctgat ggtgctgttg gaaaacatcc cggaacagga
      721 gtcgcgtcca aaacgtgaaa accagagttt gcttaatgga cctcaggtcg ataccagcaa
      781 agccggtgtg gtagccagtc aggatcaggt ggacgatttg ttggatagtc ttggattttg
      841 atttgtattg cctgatgtgg cgtgaccacg tcatatcagg cggtactaga gtcacactgg
      901 ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga
      961 ttattaatcc ggctttttta ttattttact agagaattgt gagcggataa caattgacat
     1021 tgtgagcgga taacaagata ctgagcacat actagagtca cacaggaaag tactagatga
     1081 gcacaaaaaa gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa
     1141 tttatgaaaa aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg
     1201 ggatggggca gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata
     1261 acgccgcatt gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg
     1321 ccagagaaat ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt
     1381 atgagtaccc tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct
     1441 ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat
     1501 tctggcttga ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc
     1561 ctgacggaat gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca
     1621 tagccagact tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg
     1681 tgtttttaca accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg
     1741 ttgtggggaa agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg
     1801 aaaactacgc tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagagt
     1861 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa
     1921 aagccagatt attaatccgg cttttttatt attt
//