##gff-version 3
##sequence-region BBa_K518008 1 1954
BBa_K518008	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K518008	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K518008	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K518008	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K518008	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K518008	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K518008	.	ribosome_entry_site	1058	1070	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K518008	.	ribosome_entry_site	1064	1067	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K518008	.	ribosome_entry_site	1058	1070	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K518008	.	engineered_region	1058	1070	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K518008	.	CDS	1077	1851	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K518008	.	engineered_tag	1827	1851	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K518008	.	CDS	1080	1787	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K518008	.	tag	1788	1820	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K518008	.	terminator	892	986	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K518008	.	terminator	941	986	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K518008	.	stem_loop	953	975	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K518008	.	engineered_region	941	986	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K518008	.	terminator	892	932	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K518008	.	stem_loop	899	918	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K518008	.	stop_codon	925	925	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K518008	.	engineered_region	892	932	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K518008	.	polyA_site	919	932	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K518008	.	ribosome_entry_site	58	70	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K518008	.	ribosome_entry_site	64	67	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K518008	.	ribosome_entry_site	58	70	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K518008	.	engineered_region	58	70	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K518008	.	terminator	1860	1954	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K518008	.	terminator	1909	1954	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K518008	.	stem_loop	1921	1943	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K518008	.	engineered_region	1909	1954	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K518008	.	terminator	1860	1900	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K518008	.	stem_loop	1867	1886	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K518008	.	stop_codon	1893	1893	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K518008	.	engineered_region	1860	1900	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K518008	.	polyA_site	1887	1900	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K518008	.	CDS	79	883	.	+	0	ID=BBa_K518005;Name=BBa_K518005
BBa_K518008	.	promoter	995	1048	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K518008	.	operator	1020	1036	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K518008	.	promoter	1037	1042	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K518008	.	engineered_region	995	1048	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K518008	.	operator	997	1013	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K518008	.	promoter	1014	1019	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
>BBa_K518008
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactagaga
tcattgctgcggcgcaagcgggggccagtggctatgtggtgaagccatttaccgccgcgacgctggaggaaaaactcaa
caaaatctttgagaaactgggcatgtgaggatgcgactatgatgcaaccatcaatcaaacctgctgacgagcattcagc
tggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggcc
attgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcgg
agcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacg
ttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggca
gatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccg
ggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatccc
ggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtg
gtagccagtcaggatcaggtggacgatttgttggatagtcttggattttgatttgtattgcctgatgtggcgtgaccac
gtcatatcaggcggtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaata
taaaaagccagattattaatccggcttttttattattttactagagaattgtgagcggataacaattgacattgtgagc
ggataacaagatactgagcacatactagagtcacacaggaaagtactagatgagcacaaaaaagaaaccattaacacaa
gagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctg
tcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgc
cgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtat
gaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttct
cacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcatt
ctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctc
gttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaac
tgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttc
cgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagta
gcttaataacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tatatactagagagagaatataaaaagccagattattaatccggcttttttattattt