LOCUS       BBa_K518009             1665 bp    DNA     linear       22-Dec-2024
DEFINITION  AspA expression cassette (no promoter)
ACCESSION   BBa_K518009
VERSION     BBa_K518009.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..1498
                     /label=AspA
     misc_feature    20..20
                     /label=G
     misc_feature    607..607
                     /label=C
     misc_feature    643..643
                     /label=G
     misc_feature    676..676
                     /label=C
     misc_feature    1018..1018
                     /label=G
     misc_feature    1144..1144
                     /label=G
     misc_feature    1499..1531
                     /label=LVA
     misc_feature    1538..1562
                     /label=Help:Barcodes
     CDS             20..1562
                     /label=BBa_C0083
     stem_loop       1578..1597
                     /label=T7 TE
     misc_feature    1604..1604
                     /label=stop
     polyA_site      1598..1611
                     /label=polya
     misc_feature    1571..1611
                     /label=BBa_B0012
     terminator      1571..1611
                     /label=BBa_B0012
     stem_loop       1632..1654
                     /label=stem_loop
     misc_feature    1620..1665
                     /label=BBa_B0011
     terminator      1620..1665
                     /label=BBa_B0011
     terminator      1571..1665
                     /label=BBa_B0014
ORIGIN
        1 tcacacagga aagtactaga tgtgtttaaa gcaaatcatt ggcagcttga aaaagaaggt
       61 tcacatgtca aacaacattc gtatcgaaga agatctgttg ggtaccaggg aagttccagc
      121 tgatgcctac tatggtgttc acactctgag agcgattgaa aacttctata tcagcaacaa
      181 caaaatcagt gatattcctg aatttgttcg cggtatggta atggttaaaa aagccgcagc
      241 tatggcaaac aaagagctgc aaaccattcc taaaagtgta gcgaatgcca tcattgccgc
      301 atgtgatgaa gtcctgaaca acggaaaatg catggatcag ttcccggtag acgtctacca
      361 gggcggcgca ggtacttccg taaacatgaa caccaacgaa gtgctggcca atatcggtct
      421 ggaactgatg ggtcaccaaa aaggtgaata tcagtacctg aacccgaacg accatgttaa
      481 caaatgtcag tccactaacg acgcctaccc gaccggtttc cgtatcgcag tttactcttc
      541 cctgattaag ctggtagatg cgattaacca actgcgtgaa ggctttgaac gtaaagctgt
      601 cgaatttcag gacatcctga aaatgggtcg tacccagctg caagacgcag taccgatgac
      661 cctcggtcag gaatttcgcg ctttcagcat cctgctgaaa gaagaagtga aaaacatcca
      721 acgtaccgct gaactgctgc tggaagttaa ccttggtgca acagcaatcg gtactggtct
      781 gaacacgccg aaagagtact ctccgctggc agtgaaaaaa ctggctgaag ttactggctt
      841 cccatgcgta ccggctgaag acctgatcga agcgacctct gactgcggcg cttatgttat
      901 ggttcacggc gcgctgaaac gcctggctgt gaagatgtcc aaaatctgta acgacctgcg
      961 cttgctctct tcaggcccac gtgccggcct gaacgagatc aacctgccgg aactgcaagc
     1021 gggctcttcc atcatgccag ctaaagtaaa cccggttgtt ccggaagtgg ttaaccaggt
     1081 atgcttcaaa gtcatcggta acgacaccac tgttaccatg gcagcagaag caggtcagct
     1141 gcaattgaac gttatggagc cggtcattgg ccaggccatg ttcgaatccg ttcacattct
     1201 gaccaacgct tgctacaacc tgctggaaaa atgcattaac ggcatcactg ctaacaaaga
     1261 agtgtgcgaa ggttacgttt acaactctat cggtatcgtt acttacctga acccgttcat
     1321 cggtcaccac aacggtgaca tcgtgggtaa aatctgtgcc gaaaccggta agagtgtacg
     1381 tgaagtcgtt ctggaacgcg gtctgttgac tgaagcggaa cttgacgata ttttctccgt
     1441 acagaatctg atgcacccgg cttacaaagc aaaacgctat actgatgaaa gcgaacaggc
     1501 tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc
     1561 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga
     1621 gagaatataa aaagccagat tattaatccg gcttttttat tattt
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