##gff-version 3
##sequence-region BBa_K518009 1 1665
BBa_K518009	.	CDS	20	1562	.	+	0	ID=BBa_C0083;Name=BBa_C0083
BBa_K518009	.	point_mutation	1018	1018	.	+	0	ID=annotation300987;Name=G;Parent=BBa_C0083
BBa_K518009	.	point_mutation	676	676	.	+	0	ID=annotation300986;Name=C;Parent=BBa_C0083
BBa_K518009	.	engineered_tag	1538	1562	.	+	0	ID=annotation2213998;Name=Help:Barcodes;Parent=BBa_C0083
BBa_K518009	.	point_mutation	643	643	.	+	0	ID=annotation300984;Name=G;Parent=BBa_C0083
BBa_K518009	.	CDS	20	1498	.	+	0	ID=annotation300981;Name=AspA;Parent=BBa_C0083
BBa_K518009	.	tag	1499	1531	.	+	0	ID=annotation300993;Name=LVA;Parent=BBa_C0083
BBa_K518009	.	point_mutation	20	20	.	+	0	ID=annotation300982;Name=G;Parent=BBa_C0083
BBa_K518009	.	point_mutation	1144	1144	.	+	0	ID=annotation300988;Name=G;Parent=BBa_C0083
BBa_K518009	.	point_mutation	607	607	.	+	0	ID=annotation300983;Name=C;Parent=BBa_C0083
BBa_K518009	.	terminator	1571	1665	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K518009	.	terminator	1571	1611	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K518009	.	stem_loop	1578	1597	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K518009	.	polyA_site	1598	1611	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K518009	.	stop_codon	1604	1604	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K518009	.	engineered_region	1571	1611	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K518009	.	terminator	1620	1665	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K518009	.	stem_loop	1632	1654	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K518009	.	engineered_region	1620	1665	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K518009	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K518009	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K518009	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K518009	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
>BBa_K518009
tcacacaggaaagtactagatgtgtttaaagcaaatcattggcagcttgaaaaagaaggttcacatgtcaaacaacatt
cgtatcgaagaagatctgttgggtaccagggaagttccagctgatgcctactatggtgttcacactctgagagcgattg
aaaacttctatatcagcaacaacaaaatcagtgatattcctgaatttgttcgcggtatggtaatggttaaaaaagccgc
agctatggcaaacaaagagctgcaaaccattcctaaaagtgtagcgaatgccatcattgccgcatgtgatgaagtcctg
aacaacggaaaatgcatggatcagttcccggtagacgtctaccagggcggcgcaggtacttccgtaaacatgaacacca
acgaagtgctggccaatatcggtctggaactgatgggtcaccaaaaaggtgaatatcagtacctgaacccgaacgacca
tgttaacaaatgtcagtccactaacgacgcctacccgaccggtttccgtatcgcagtttactcttccctgattaagctg
gtagatgcgattaaccaactgcgtgaaggctttgaacgtaaagctgtcgaatttcaggacatcctgaaaatgggtcgta
cccagctgcaagacgcagtaccgatgaccctcggtcaggaatttcgcgctttcagcatcctgctgaaagaagaagtgaa
aaacatccaacgtaccgctgaactgctgctggaagttaaccttggtgcaacagcaatcggtactggtctgaacacgccg
aaagagtactctccgctggcagtgaaaaaactggctgaagttactggcttcccatgcgtaccggctgaagacctgatcg
aagcgacctctgactgcggcgcttatgttatggttcacggcgcgctgaaacgcctggctgtgaagatgtccaaaatctg
taacgacctgcgcttgctctcttcaggcccacgtgccggcctgaacgagatcaacctgccggaactgcaagcgggctct
tccatcatgccagctaaagtaaacccggttgttccggaagtggttaaccaggtatgcttcaaagtcatcggtaacgaca
ccactgttaccatggcagcagaagcaggtcagctgcaattgaacgttatggagccggtcattggccaggccatgttcga
atccgttcacattctgaccaacgcttgctacaacctgctggaaaaatgcattaacggcatcactgctaacaaagaagtg
tgcgaaggttacgtttacaactctatcggtatcgttacttacctgaacccgttcatcggtcaccacaacggtgacatcg
tgggtaaaatctgtgccgaaaccggtaagagtgtacgtgaagtcgttctggaacgcggtctgttgactgaagcggaact
tgacgatattttctccgtacagaatctgatgcacccggcttacaaagcaaaacgctatactgatgaaagcgaacaggct
gcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggctttttta
ttattt