LOCUS       BBa_K518012              828 bp    DNA     linear       03-May-2024
DEFINITION  RBS + RFP + d.Ter
ACCESSION   BBa_K518012
VERSION     BBa_K518012.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..694
                     /label=mrfp1
     misc_feature    701..725
                     /label=Help:Barcodes
     CDS             20..725
                     /label=BBa_E1010
     stem_loop       741..760
                     /label=T7 TE
     misc_feature    767..767
                     /label=stop
     polyA_site      761..774
                     /label=polya
     misc_feature    734..774
                     /label=BBa_B0012
     terminator      734..774
                     /label=BBa_B0012
     stem_loop       795..817
                     /label=stem_loop
     misc_feature    783..828
                     /label=BBa_B0011
     terminator      783..828
                     /label=BBa_B0011
     terminator      734..828
                     /label=BBa_B0014
ORIGIN
        1 tcacacagga aagtactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
       61 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
      121 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
      181 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
      241 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
      301 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
      361 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
      421 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
      481 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
      541 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
      601 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
      661 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag
      721 atcgctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
      781 agagagaata taaaaagcca gattattaat ccggcttttt tattattt
//