LOCUS       BBa_K518013             2944 bp    DNA     linear       11-Dec-2025
DEFINITION  sulA promoter evaluation device
ACCESSION   BBa_K518013
VERSION     BBa_K518013.1  
FEATURES             Location/Qualifiers
     promoter        1..52
                     /label=BBa_K518010
     RBS             61..73
                     /label=RBS-3\Weak
     RBS             67..70
                     /label=RBS
     misc_feature    61..73
                     /label=BBa_B0032
     RBS             61..73
                     /label=BBa_B0032
     CDS             80..1732
                     /label=BBa_I712019
     stem_loop       1748..1767
                     /label=T7 TE
     misc_feature    1774..1774
                     /label=stop
     polyA_site      1768..1781
                     /label=polya
     misc_feature    1741..1781
                     /label=BBa_B0012
     terminator      1741..1781
                     /label=BBa_B0012
     stem_loop       1802..1824
                     /label=stem_loop
     misc_feature    1790..1835
                     /label=BBa_B0011
     terminator      1790..1835
                     /label=BBa_B0011
     terminator      1741..1835
                     /label=BBa_B0014
     promoter        1844..1878
                     /label=BBa_J23118
     RBS             1887..1899
                     /label=RBS-3\Weak
     RBS             1893..1896
                     /label=RBS
     misc_feature    1887..1899
                     /label=BBa_B0032
     RBS             1887..1899
                     /label=BBa_B0032
     misc_feature    1906..2841
                     /label=Rluc
     CDS             1906..2841
                     /label=Rluc
     misc_feature    1906..2841
                     /label=BBa_J52008
     stem_loop       2857..2876
                     /label=T7 TE
     misc_feature    2883..2883
                     /label=stop
     polyA_site      2877..2890
                     /label=polya
     misc_feature    2850..2890
                     /label=BBa_B0012
     terminator      2850..2890
                     /label=BBa_B0012
     stem_loop       2911..2933
                     /label=stem_loop
     misc_feature    2899..2944
                     /label=BBa_B0011
     terminator      2899..2944
                     /label=BBa_B0011
     terminator      2850..2944
                     /label=BBa_B0014
     misc_feature    61..2944
                     /label=BBa_K518002
ORIGIN
        1 gggttgatct ttgttgtcac tggatgtact gtacatccat acagtaactc actactagag
       61 tcacacagga aagtactaga tggaagacgc caaaaacata aagaaaggcc cggcgccatt
      121 ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
      181 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc
      241 tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
      301 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
      361 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
      421 gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca
      481 aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc
      541 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
      601 cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact
      661 gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac
      721 tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
      781 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
      841 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct
      901 gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt
      961 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
     1021 tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc
     1081 aggtatcagg caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     1141 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     1201 ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg
     1261 tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     1321 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     1381 cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt
     1441 ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga
     1501 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     1561 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     1621 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     1681 aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt aatactagag
     1741 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa
     1801 aaagccagat tattaatccg gcttttttat tattttacta gagttgacgg ctagctcagt
     1861 cctaggtatt gtgctagcta ctagagtcac acaggaaagt actagatggc ttccaaggtg
     1921 tacgaccccg agcaacgcaa acgcatgatc actgggcctc agtggtgggc tcgctgcaag
     1981 caaatgaacg tgctggactc cttcatcaac tactatgatt ccgagaagca cgccgagaac
     2041 gccgtgattt ttctgcatgg taacgctgcc tccagctacc tgtggaggca cgtcgtgcct
     2101 cacatcgagc ccgtggctag atgcatcatc cctgatctga tcggaatggg taagtccggc
     2161 aagagcggga atggctcata tcgcctcctg gatcactaca agtacctcac cgcttggttc
     2221 gagctgctga accttccaaa gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg
     2281 gcctttcact actcctacga gcaccaagac aagatcaagg ccatcgtcca tgctgagagt
     2341 gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca tcgaggagga tatcgccctg
     2401 atcaagagcg aagagggcga gaaaatggtg cttgagaata acttcttcgt cgagaccatg
     2461 ctcccaagca agatcatgcg gaaactggag cctgaggagt tcgctgccta cctggagcca
     2521 ttcaaggaga agggcgaggt tagacggcct accctctcct ggcctcgcga gatccctctc
     2581 gttaagggag gcaagcccga cgtcgtccag attgtccgca actacaacgc ctaccttcgg
     2641 gccagcgacg atctgcctaa gatgttcatc gagtccgacc ctgggttctt ttccaacgct
     2701 attgtcgagg gagctaagaa gttccctaac accgagttcg tgaaggtgaa gggcctccac
     2761 ttcagccagg aggacgctcc agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc
     2821 gtgctgaaga acgagcagta atactagagt cacactggct caccttcggg tgggcctttc
     2881 tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg cttttttatt
     2941 attt
//