BBa_K518013
type: ComponentDefinition
persistent identity: BBa K518013
display id: BBa_K518013
version: 1
was generated by: iGEM to SBOL conversion
description: sulA promoter evaluation device
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:33Z
mutable provenance: sulAp (BBa_K518010) was cloned from Escherichia coli K12 strain's genome.
owned by: james
owned by: myers
mutable description: Microbes including Escherichia coli are known to respond to various DNA-injuring stress (ionizing radiation, ultraviolet radiation, peroxides etc...), altering their gene expressions. This response, known as "SOS response", are induced by regulatory protein called RecA, which is activated when binds to single-strand DNA. DNA-RecA complex promotes the self-degradation of LexA, a common repressor for SOS genes. SulA is responsible for stress-induced halt of cell division. The promoter of sulA, or sulAp, is acutely induced by various stress factors, including ultraviolet irradiation. We provide a sulAp evaluation device to make it easy to measure the varying expression of sulAp in various "stressful" conditions. Employing a dual luciferase reporter construct, quantitative measurement and comparison of Relative Promoter Unit (RPU) is achieved.
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: sulAp + dual luciferase reporter construct
star: false
creator: Masato Ohgishi
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_K518002
component: BBa_K518010
sequence annotation: BBa_K518010
sequence annotation: BBa_K518002
sequence: https://synbiohub.org/public/igem/BBa_K518013_sequence/1

BBa_K518002
type: Component
persistent identity: component2151421
display id: component2151421
version: 1
top level: BBa_K518013
definition: BBa_K518002
access: public

BBa_K518010
type: Component
persistent identity: component2151395
display id: component2151395
version: 1
top level: BBa_K518013
definition: sulAp
access: public

BBa_K518010
type: SequenceAnnotation
persistent identity: annotation2151395
display id: annotation2151395
version: 1
top level: BBa_K518013
location: https://synbiohub.org/public/igem/BBa_K518013/annotation2151395/range2151395/1
component: BBa_K518010

https://synbiohub.org/public/igem/BBa_K518013/annotation2151395/range2151395/1
type: Range
persistent identity: range2151395
display id: range2151395
version: 1
top level: BBa_K518013
direction: direction/reverse
start: 1
end: 52
orientation: inline

BBa_K518002
type: SequenceAnnotation
persistent identity: annotation2151421
display id: annotation2151421
version: 1
top level: BBa_K518013
location: https://synbiohub.org/public/igem/BBa_K518013/annotation2151421/range2151421/1
component: BBa_K518002

https://synbiohub.org/public/igem/BBa_K518013/annotation2151421/range2151421/1
type: Range
persistent identity: range2151421
display id: range2151421
version: 1
top level: BBa_K518013
direction: direction/reverse
start: 61
end: 2944
orientation: inline

sulAp
type: ComponentDefinition
persistent identity: BBa K518010
display id: BBa_K518010
version: 1
was generated by: iGEM to SBOL conversion
description: sulA promoter
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:33Z
mutable provenance: Cloned from E. coli K12 strain genome.
owned by: james
owned by: myers
mutable description: Microbes including Escherichia coli are known to respond to various DNA-injuring stress (ionizing radiation, ultraviolet radiation, peroxides etc...), altering their gene expressions. This response, known as "SOS response", are induced by regulatory protein called RecA, which is activated when binds to single-strand DNA. DNA-RecA complex promotes the self-degradation of LexA, a common repressor for SOS genes. SulA is responsible for stress-induced halt of cell division. The promoter of sulA, or sulAp, is acutely induced by various stress factors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None.
star: false
creator: Masato Ohgishi
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K518010_sequence/1

BBa_K518002
type: ComponentDefinition
persistent identity: BBa K518002
display id: BBa_K518002
version: 1
was generated by: iGEM to SBOL conversion
description: Firefly-renilla dual luciferase assay kit
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:33Z
mutable provenance: From BioBrick parts.
owned by: james
owned by: myers
mutable description: Luciferase assay is one of the most popular reporter assay system for quantitatively measuring the function of promoters and other cis-elements. The wide-ranging and quantitative detection is the prominent feature of this assay; light emmision from 10~(-18) ― 10~(-11) mol protein can be quantitatively measured using luminometer (see our results). A promoter or other cis-element to be analysed can be ligated above this part. Its effect will be measured as an altered firefly luciferase expression. Meanwhile, renilla luciferase expression can be used to as an internal control.
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Masato Ohgishi
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_B0014
component: BBa_I712019
component: BBa_B0032
component: BBa_J52008
component: BBa_J23118
sequence annotation: BBa_B0032
sequence annotation: BBa_I712019
sequence annotation: BBa_B0014
sequence annotation: BBa_J23118
sequence annotation: BBa_J52008
sequence: https://synbiohub.org/public/igem/BBa_K518002_sequence/1

BBa_B0014
type: Component
persistent identity: component2128885
display id: component2128885
version: 1
top level: BBa_K518002
definition: BBa_B0014
access: public

BBa_I712019
type: Component
persistent identity: component2128878
display id: component2128878
version: 1
top level: BBa_K518002
definition: BBa_I712019
access: public

BBa_B0032
type: Component
persistent identity: component2128876
display id: component2128876
version: 1
top level: BBa_K518002
definition: BBa_B0032
access: public

BBa_B0014
type: Component
persistent identity: component2128899
display id: component2128899
version: 1
top level: BBa_K518002
definition: BBa_B0014
access: public

BBa_J52008
type: Component
persistent identity: component2128892
display id: component2128892
version: 1
top level: BBa_K518002
definition: BBa_J52008
access: public

BBa_B0032
type: Component
persistent identity: component2128888
display id: component2128888
version: 1
top level: BBa_K518002
definition: BBa_B0032
access: public

BBa_J23118
type: Component
persistent identity: component2128886
display id: component2128886
version: 1
top level: BBa_K518002
definition: BBa_J23118
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128876
display id: annotation2128876
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128876/range2128876/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518002/annotation2128876/range2128876/1
type: Range
persistent identity: range2128876
display id: range2128876
version: 1
top level: BBa_K518002
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_I712019
type: SequenceAnnotation
persistent identity: annotation2128878
display id: annotation2128878
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128878/range2128878/1
component: BBa_I712019

https://synbiohub.org/public/igem/BBa_K518002/annotation2128878/range2128878/1
type: Range
persistent identity: range2128878
display id: range2128878
version: 1
top level: BBa_K518002
direction: direction/reverse
start: 20
end: 1672
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2128885
display id: annotation2128885
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128885/range2128885/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518002/annotation2128885/range2128885/1
type: Range
persistent identity: range2128885
display id: range2128885
version: 1
top level: BBa_K518002
direction: direction/reverse
start: 1681
end: 1775
orientation: inline

BBa_J23118
type: SequenceAnnotation
persistent identity: annotation2128886
display id: annotation2128886
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128886/range2128886/1
component: BBa_J23118

https://synbiohub.org/public/igem/BBa_K518002/annotation2128886/range2128886/1
type: Range
persistent identity: range2128886
display id: range2128886
version: 1
top level: BBa_K518002
direction: direction/reverse
start: 1784
end: 1818
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128888
display id: annotation2128888
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128888/range2128888/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518002/annotation2128888/range2128888/1
type: Range
persistent identity: range2128888
display id: range2128888
version: 1
top level: BBa_K518002
direction: direction/forward
start: 1827
end: 1839
orientation: inline

BBa_J52008
type: SequenceAnnotation
persistent identity: annotation2128892
display id: annotation2128892
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128892/range2128892/1
component: BBa_J52008

https://synbiohub.org/public/igem/BBa_K518002/annotation2128892/range2128892/1
type: Range
persistent identity: range2128892
display id: range2128892
version: 1
top level: BBa_K518002
direction: direction/reverse
start: 1846
end: 2781
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2128899
display id: annotation2128899
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128899/range2128899/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518002/annotation2128899/range2128899/1
type: Range
persistent identity: range2128899
display id: range2128899
version: 1
top level: BBa_K518002
direction: direction/reverse
start: 2790
end: 2884
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_I712019
type: ComponentDefinition
persistent identity: BBa I712019
display id: BBa_I712019
version: 1
was generated by: iGEM to SBOL conversion
description: Firefly luciferase - luciferase from Photinus pyralis
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
mutable provenance: This firefly luciferase comes from Promega PGL3 vector.
owned by: james
owned by: myers
mutable description: Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.
discontinued: false
dominant: true
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Part is cloned into pSBAK3 vector with terminator.
star: true
creator: Anja Korenčič
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_I712019_sequence/1

BBa_J52008
type: ComponentDefinition
persistent identity: BBa J52008
display id: BBa_J52008
version: 1
was generated by: iGEM to SBOL conversion
description: luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
mutable provenance: -
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Rluc is a protein called Renilla`s luciferase and it emits light when adding the right substrate. That is why it can bi used as a reported to track other proteins or to monitor the activity of a promotor.
discontinued: false
dominant: true
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Rluc is cloned in BioBrick vector pSB1AK3.
star: true
creator: Monika Ciglic
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52008_sequence/1

Rluc
type: SequenceAnnotation
persistent identity: annotation1902845
display id: annotation1902845
version: 1
top level: BBa_J52008
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1
type: Range
persistent identity: range1902845
display id: range1902845
version: 1
top level: BBa_J52008
direction: direction/forward
start: 1
end: 936
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902833
display id: annotation1902833
version: 1
top level: BBa_J52008
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1
type: Range
persistent identity: range1902833
display id: range1902833
version: 1
top level: BBa_J52008
direction: direction/forward
start: 1
end: 936
orientation: inline

BBa_J23118
type: ComponentDefinition
persistent identity: BBa J23118
display id: BBa_J23118
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23118_sequence/1

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K518013_sequence/1
type: Sequence
persistent identity: BBa K518013 sequence
display id: BBa_K518013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggttgatctttgttgtcactggatgtactgtacatccatacagtaactcactactagagtcacacaggaaagtactagatggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagttgacggctagctcagtcctaggtattgtgctagctactagagtcacacaggaaagtactagatggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K518010_sequence/1
type: Sequence
persistent identity: BBa K518010 sequence
display id: BBa_K518010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggttgatctttgttgtcactggatgtactgtacatccatacagtaactcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K518002_sequence/1
type: Sequence
persistent identity: BBa K518002 sequence
display id: BBa_K518002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagttgacggctagctcagtcctaggtattgtgctagctactagagtcacacaggaaagtactagatggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I712019_sequence/1
type: Sequence
persistent identity: BBa I712019 sequence
display id: BBa_I712019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J52008_sequence/1
type: Sequence
persistent identity: BBa J52008 sequence
display id: BBa_J52008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23118_sequence/1
type: Sequence
persistent identity: BBa J23118 sequence
display id: BBa_J23118_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtattgtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z