LOCUS BBa_K519003 3783 bp DNA linear 29-Nov-2024 DEFINITION Inducible BMC shell protein generator with GFP fused pduP1~18 tag ACCESSION BBa_K519003 VERSION BBa_K519003.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 64..75 /label=BBa_B0034 misc_feature 2678..2806 /label=BBa_B0015 CDS 82..363 /label=pduA CDS 363..1175 /label=pduB misc_feature 1190..1201 /label=BBa_B0034 CDS 1208..1483 /label=pduJ CDS 1508..1972 /label=pduK misc_feature 1987..1998 /label=BBa_B0034 CDS 2005..2280 /label=pduN misc_feature 2295..2306 /label=BBa_B0034 CDS 2313..2663 /label=pduU misc_feature 64..2806 /label=BBa_K317041 misc_feature 1..2806 /label=BBa_K519002 misc_feature 2815..2849 /label=BBa_J23102 misc_feature 2858..2869 /label=BBa_B0034 CDS 2876..3646 /label=pduP-GFPuv fusion misc_feature 3655..3783 /label=BBa_B0015 misc_feature 2815..3783 /label=BBa_K317030 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgcaacaa gaagcgttag gaatggtaga aaccaaaggc 121 ttgactgcgg ccatagaggc cgcagatgca atggtgaagt cagccaatgt aatgctggtc 181 ggctacgaaa aaattggttc ggggctggta acagttattg tccgcggcga tgtcggcgca 241 gtcaaagcag caacagatgc cggtgccgcc gcagcccgta atgtgggaga agtgaaagcc 301 gtacacgtta tcccacgccc gcataccgat gtagaaaaaa tcttaccgaa gggaattagc 361 taatgagcag caatgaactg gttgaacaga tcatggcgca ggtgattgct cgcgtggcaa 421 cgccggaaca gcaggctatc ccagaaaata atcacccaac acgagaaacg gctatggcag 481 agaaaagctg tagtttaacg gagtttgtcg gtaccgcgat tggcgacacc gtcggtctgg 541 taatcgccaa cgtagacagt gccctactgg acgcaatgaa acttgaaaaa cgctatcgct 601 ccattggcat ccttggcgcc cgtactggtg cgggtccaca catcatggcc gctgatgaag 661 cggtgaaagc caccaatact gaagtcgtca gtattgagtt accacgtgat accaaaggcg 721 gcgcaggtca tggctcgctg attattcttg gcggcaacga tgtttccgac gtgaaacgcg 781 gcattgaagt cgcactgaaa gagctggatc gcacctttgg cgacgtttat gccaacgaag 841 ccggtcacat cgaaatgcaa tacaccgcac gcgccagcta cgcacttgaa aaagcctttg 901 gcgcaccgat tggtcgtgcc tgtggcgtca tcgtcggcgc accagcatcc gttggtgtcc 961 tgatggctga tactgccctg aaatccgcca acgtggaagt tgtggcttac agctcccctg 1021 ctcatggcac cagcttcagt aacgaagcca ttctggttat ttcgggtgac tctggcgctg 1081 ttcgtcaggc tgttatctct gcccgtgaaa tcggtaaaac cgtactcggg accctcggct 1141 cagagccgaa aaacgatcgt ccgtcctaca tctgataata atactagaga aagaggagaa 1201 atactagatg aataacgcac tgggactggt tgaaacaaaa gggcttgtcg gcgctattga 1261 agccgctgat gccatggtga aatccgcaaa cgtgcagttg gttggttacg aaaaaatcgg 1321 ctctggcctg atcaccgtta tggttcgcgg tgatgtcggc gcggtgaaag cagccgtaga 1381 tgcaggaagc gctgcggcaa gcgccgttgg tgaggtgaaa tcctgccacg ttatcccgcg 1441 tccgcacagc gacgttgaag ccattttacc taaatccgca taagtcgttg caaccaagga 1501 gcacagcgtg aagcaatcac tgggattact tgaagttagt ggtctggcat tagccatcag 1561 ttgcgcggac gtcatggcga aagccgcctc catcacgctg gtgggcctcg aaaaaaccaa 1621 cggctcaggc tggacggtga tcaagataat cggggatgtg gcctccgtcc aggcggccat 1681 ttccactggt gtcagtttcg ctgaccagcg agatggactg gtggctcaca aagtcatttc 1741 cagacccggg gacgggatcc tgtcgcatag cgttgtcctg gagcctgaac caacgcccga 1801 ccccatacca gccataccgc atgaagagat atttgtagac catgcggcac ccgaagcacc 1861 gcaagatgca gaattgatta gctgcaattt gtgtcttgat cctgcctgcc ctcgccaaaa 1921 aggtgagccg cgcacacttt gtcttcactc cggcaaacga ggtgaagcgt gataataata 1981 ctagagaaag aggagaaata ctagatgcat ctggcacggg ttacaggcgt tgtggtttcc 2041 acgcaaaaat ctccatcact ggtgggaaaa aaactgttgc tggtacgtcg ggtaagtgct 2101 gacggagagc ttcctgcgtc tccagtgagt ggagatgaag tcgctgttga ttctgtcggc 2161 gcggggaccg gcgaactggt attactcagc agcgggtcca gcgccagaca cgttttttcc 2221 ggcccgaatg aggccattga tctggctatc gtcggcattg tcgacacgct ttctcgttag 2281 taataatact agagaaagag gagaaatact agatggaaag acaacccacc acggatcgta 2341 tgattcagga atatgttcct ggcaaacagg tcacgttggc gcaccttatc gccaatcccg 2401 gtaaggattt gttcaagaaa ctgggattac ctgattcggt ttctgcgatc ggtattctga 2461 ccattacccc tagcgaagcc tcaatcatcg cctgtgatat cgccacgaaa tcaggggcgg 2521 tagagattgg ttttctcgac cggtttaccg gcgcggtggt actgacgggc gatgtttcag 2581 ccgttgagta cgcgctgaaa caggtaaccc gaacgctggg cgaaatgatg cgttttaccg 2641 cgtgtcccat cacccggaca taataataat actagagcca ggcatcaaat aaaacgaaag 2701 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 2761 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagttgaca 2821 gctagctcag tcctaggtac tgtgctagct actagagaaa gaggagaaat actagatgaa 2881 cacttcagaa cttgaaaccc ttattcgtaa cattttgagt gagcaactta tgagtaaagg 2941 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg 3001 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct 3061 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt 3121 ctcttatggt gttcaatgct tttcccgtta tccggatcat atgaaacggc atgacttttt 3181 caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca aagatgacgg 3241 gaactacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta atcgtatcga 3301 gttaaaaggt attgatttta aagaagatgg aaacattctc ggacacaaac tcgagtacaa 3361 ctataactca cacaatgtat acatcacggc agacaaacaa aagaatggaa tcaaagctaa 3421 cttcaaaatt cgccacaaca ttgaagatgg atccgttcaa ctagcagacc attatcaaca 3481 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtcgacaca 3541 atctgccctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt 3601 aactgctgct gggattacac atggcatgga tgagctctac aaataatact agagccaggc 3661 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 3721 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 3781 ata //