LOCUS       BBa_K519020             2835 bp    DNA     linear       22-Nov-2024
DEFINITION  Plac-Lysis device without antiholin
ACCESSION   BBa_K519020
VERSION     BBa_K519020.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1147
                     /label=BBa_I732100
     stem_loop       1167..1210
                     /label=stem_loop
     misc_feature    1156..1235
                     /label=BBa_B0010
     terminator      1156..1235
                     /label=BBa_B0010
     stem_loop       1251..1270
                     /label=T7 TE
     misc_feature    1277..1277
                     /label=stop
     polyA_site      1271..1284
                     /label=polya
     misc_feature    1244..1284
                     /label=BBa_B0012
     terminator      1244..1284
                     /label=BBa_B0012
     terminator      1156..1284
                     /label=BBa_B0015
     CDS             1293..1380
                     /label=end of LacI coding region (inactive)
     misc_feature    1293..1492
                     /label=BBa_R0010
     misc_feature    1381..1418
                     /label=CAP binding site
     promoter        1429..1434
                     /label=-35
     promoter        1453..1458
                     /label=-10
     misc_feature    1458..1492
                     /label=LacI binding site
     misc_feature    1465..1465
                     /label=start
     promoter        1293..1492
                     /label=BBa_R0010
     CDS             1517..2173
                     /label=T4 holin
     CDS             1501..2176
                     /label=BBa_K112805
     CDS             2201..2695
                     /label=T4 Endolysin
     CDS             2185..2698
                     /label=BBa_K112806
     stem_loop       2718..2761
                     /label=stem_loop
     misc_feature    2707..2786
                     /label=BBa_B0010
     terminator      2707..2786
                     /label=BBa_B0010
     stem_loop       2802..2821
                     /label=T7 TE
     misc_feature    2828..2828
                     /label=stop
     polyA_site      2822..2835
                     /label=polya
     misc_feature    2795..2835
                     /label=BBa_B0012
     terminator      2795..2835
                     /label=BBa_B0012
     terminator      2707..2835
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
      121 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
      181 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
      241 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
      301 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
      361 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
      421 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
      481 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
      541 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
      601 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
      661 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
      721 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
      781 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
      841 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
      901 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
      961 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     1021 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     1081 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     1141 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1201 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1261 cgggtgggcc tttctgcgtt tatatactag agcaatacgc aaaccgcctc tccccgcgcg
     1321 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     1381 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     1441 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag
     1501 cttaaaagga gggtctatgg cagcacctag aatatcattt tcgccctctg atattctatt
     1561 tggtgttctc gatcgcttgt tcaaagataa cgctaccggg aaggttcttg cttcccgggt
     1621 agctgtcgta attcttttgt ttataatggc gattgtttgg tataggggag atagtttctt
     1681 tgagtactat aagcaatcaa agtatgaaac atacagtgaa attattgaaa aggaaagaac
     1741 tgcacgcttt gaatctgtcg ccctggaaca actccagata gttcatatat catctgaggc
     1801 agactttagt gcggtgtatt ctttccgccc taaaaactta aactattttg ttgatattat
     1861 agcatacgaa ggaaaattac cttcaacaat aagtgaaaaa tcacttggag gatatcctgt
     1921 tgataaaact atggatgaat atacagttca tttaaatgga cgtcattatt attccaactc
     1981 aaaatttgct tttttaccaa ctaaaaagcc tactcccgaa ataaactaca tgtacagttg
     2041 tccatatttt aatttggata atatctatgc tggaacgata accatgtact ggtatagaaa
     2101 tgatcatata agtaatgacc gccttgaatc aatatgtgct caggcggcca gaatattagg
     2161 aagggctaaa taattatact agagatactt aggaggtatt atgaatatat ttgaaatgtt
     2221 acgtatagat gaaggtctta gacttaaaat ctataaagac acagaaggct attacactat
     2281 tggcatcggt catttgctta caaaaagtcc atcacttaat gctgctaaat ctgaattaga
     2341 taaagctatt gggcgtaatt gcaatggtgt aattacaaaa gatgaggctg aaaaactctt
     2401 taatcaggat gttgatgctg ctgttcgcgg aatcctgaga aatgctaaat taaaaccggt
     2461 ttatgattct cttgatgcgg ttcgtcgctg tgcattgatt aatatggttt tccaaatggg
     2521 agaaaccggt gtggcaggat ttactaactc tttacgtatg cttcaacaaa aacgctggga
     2581 tgaagcagca gttaacttag ctaaaagtag atggtataat caaacaccta atcgcgcaaa
     2641 acgagtcatt acaacgttta gaactggcac ttgggacgcg tataaaaatc tataaagcta
     2701 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2761 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2821 ctttctgcgt ttata
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