LOCUS       BBa_K523000              556 bp    DNA     linear       26-Dec-2024
DEFINITION  Plac + lacZ; with BglII site
ACCESSION   BBa_K523000
VERSION     BBa_K523000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..4
                     /label=BglII
     RBS             315..318
                     /label=rbs
     CDS             326..556
                     /label=LacZ'
     misc_feature    204..241
                     /label=CAP binding site
     promoter        253..258
                     /label=-35
     promoter        276..281
                     /label=-10
     misc_feature    288..308
                     /label=LacI binding site
     misc_feature    326..326
                     /label=ATG
     misc_feature    554..554
                     /label=TGA
ORIGIN
        1 atctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
       61 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
      121 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
      181 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      241 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtgaaat tgtgagcgga
      301 taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt
      361 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      421 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      481 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga
      541 aagctggctg gagtga
//