LOCUS       BBa_K523001             2067 bp    DNA     linear       04-May-2024
DEFINITION  RBS + malS (E. coli periplasmic α-amylase)
ACCESSION   BBa_K523001
VERSION     BBa_K523001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..4
                     /label=BglII
     RBS             20..23
                     /label=Native RBS
     misc_feature    34..34
                     /label=ATG
     CDS             34..2067
                     /label=malS
     misc_feature    2062..2062
                     /label=TAATAA
     misc_feature    34..84
                     /label=Predicted (cleaved) signal peptide
ORIGIN
        1 atctgaaatc gcagcaataa ggactcatcc gccatgaaac tcgccgcctg ttttctgaca
       61 ctccttcctg gcttcgccgt tgccgccagc tggacttctc cggggtttcc cgcctttagc
      121 gaacagggga caggaacatt tgtcagccac gcgcagttgc ccaaaggtac gcgtccacta
      181 acgctaaatt ttgaccaaca gtgctggcag cctgcggatg cgataaaact caatcagatg
      241 ctttccctgc aaccttgtag caacacgccg cctcaatggc gattgttcag ggacggcgaa
      301 tatacgctgc aaatagacac ccgctccggt acgccaacat tgatgatttc catccagaac
      361 gccgccgaac cggtagcaag cctggtccgt gaatgcccga aatgggatgg attaccgctc
      421 acagtggatg tcagcgccac tttcccggaa ggagccgccg tacgggatta ttacagccag
      481 caaattgcga tagtgaagaa cggtcaaata atgttacaac ccgctgccac cagcaacggt
      541 ttactcctgc tggaacgggc agaaactgac acatccgccc ctttcgactg gcataacgcc
      601 acggtttact ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa tgaccagagt
      661 tacggacgtc ataaagacgg tatggcggaa attggcactt ttcacggcgg cgatttacgc
      721 ggcctgacca acaaactgga ttacctccag cagttgggcg ttaatgcttt atggataagc
      781 gccccatttg agcaaattca cggctgggtc ggcggcggta caaaaggcga tttcccgcat
      841 tatgcctacc acggttatta cacacaggac tggacgaatc ttgatgccaa tatgggcaac
      901 gaagccgatc tacggacgct ggttgatagc gcacatcagc gcggtattcg tattctcttt
      961 gatgtcgtga tgaaccacac cggctatgcc acgctggcgg atatgcagga gtatcagttt
     1021 ggcgcgttat atctttctgg tgacgaagtg aaaaaatcgc tgggtgaacg ctggagcgac
     1081 tggaaacctg ccgccgggca aacctggcat agctttaacg attacattaa tttcagcgac
     1141 aaaacaggct gggataaatg gtggggaaaa aactggatca gaacggatat cggcgattac
     1201 gacaatcctg gattcgacga tctcactatg tcgctagcct ttttgccgga tatcaaaacc
     1261 gaatcaacta ccgcttctgg tctgccggtg ttctataaaa acaaaatgga tacccacgcc
     1321 aaagccattg acggctatac gccgcgcgat tacttaaccc actggttaag tcagtgggtc
     1381 cgcgactatg ggattgatgg ttttcgggtc gataccgcca aacatgttga gttgcccgcc
     1441 tggcagcaac tgaaaaccga agccagcgcc gcgcttcgcg aatggaaaaa agctaacccc
     1501 gacaaagcat tagatgacaa acctttctgg atgaccggtg aagcctgggg ccacggcgtg
     1561 atgcaaagtg actactatcg ccacggcttc gatgcgatga tcaatttcga ttatcaggag
     1621 caggcggcga aagcagtcga ctgtctggcg cagatggata cgacctggca gcaaatggcg
     1681 gagaaattgc agggtttcaa cgtgttgagc tacctctcgt cgcatgatac ccgcctgttc
     1741 cgtgaagggg gcgacaaagc agcagagtta ttactattag cgccaggcgc ggtacaaatc
     1801 ttttatggtg atgaatcctc gcgtccgttc ggtcctacag gttctgatcc gctgcaaggt
     1861 acacgttcgg atatgaactg gcaggatgtt agcggtaaat ctgccgccag cgtcgcgcac
     1921 tggcagaaaa tcagccagtt ccgcgcccgc catcccgcaa ttggcgcggg caaacaaacg
     1981 acacttttgc tgaagcaggg ctacggcttt gttcgtgagc atggcgacga taaagtgctg
     2041 gtcgtctggg cagggcaaca gtaataa
//