LOCUS BBa_K523002 2338 bp DNA linear 22-Dec-2024 DEFINITION RBS + bglX (E. coli perisplasmic β-glucosidase) ACCESSION BBa_K523002 VERSION BBa_K523002.1 FEATURES Location/Qualifiers misc_feature 1..4 /label=BglII misc_feature 22..25 /label=Native RBS CDS 38..2338 /label=bglX misc_feature 38..38 /label=ATG misc_feature 2333..2333 /label=TAATAA misc_feature 85..85 /label=PstI site removed misc_feature 2122..2122 /label=G to T silent mutation misc_feature 38..97 /label=Predicted (cleaved) signal peptide ORIGIN 1 atctgccacg tcgggcaaca aaggaagaaa aatccatatg aaatggctat gttcagtagg 61 aatcgcggtg agtctggccc tgcaaccagc actggcggat gatttattcg gcaaccatcc 121 attaacgccc gaagcgcggg atgcgttcgt caccgaactg cttaagaaaa tgacagttga 181 tgagaaaatt ggtcagctgc gcttaatcag cgtcggcccg gataacccga aagaggcgat 241 ccgcgagatg atcaaagacg gtcaggttgg ggcgattttc aacaccgtaa cccgtcagga 301 tatccgcgcc atgcaggatc aggtgatgga attaagccgc ctgaaaattc ctcttttctt 361 tgcttacgac gtgctgcacg gtcagcgcac ggtgttcccg attagcctcg gtctggcctc 421 gtcttttaac ctcgatgcag tgaaaacggt cggacgtgtc tctgcttatg aagcggcaga 481 tgatggcctg aatatgacct gggcaccgat ggtcgatgtc tcgcgcgatc cgcgctgggg 541 acgtgcttcc gaaggttttg gcgaagatac gtatctcacc tcaacaatgg gtaaaaccat 601 ggtggaagcg atgcagggta aaagcccggc agatcgctac tcggtgatga ccagcgtcaa 661 acactttgcc gcatacggcg cggtagaagg cggtaaagag tacaacaccg tcgatatgag 721 tccgcagcgc ctgtttaatg attatatgcc gccgtacaaa gcggggctgg acgcaggcag 781 cggcgcggtg atggtggcgc tgaactcgct gaacggcacg ccagccacct ccgattcctg 841 gctgctgaaa gatgttctgc gcgaccagtg gggctttaaa ggcatcaccg tttccgatca 901 cggtgcaatc aaagagctga ttaaacatgg cacggcggca gacccggaag atgcggtgcg 961 cgtggcgctg aaatccggaa tcaacatgag catgagcgac gagtactact cgaagtatct 1021 gcctgggttg attaaatccg gcaaagtgac gatggcagag ctggacgatg ctgcccgcca 1081 tgtactgaac gttaaatatg atatggggtt gtttaacgac ccatacagcc atttggggcc 1141 gaaagagtct gacccggtgg ataccaatgc cgaaagccgc ctgcaccgta aagaagcgcg 1201 tgaagtggcg cgcgaaagct tggtgttgct gaaaaaccgt ctcgaaacgt taccgctgaa 1261 aaaatcggcc accattgcgg tggttgggcc actggcggac agtaaacgtg acgtgatggg 1321 cagctggtcc gcagccggtg ttgccgatca atccgtgacc gtactgaccg ggattaaaaa 1381 tgccgtcggt gaaaacggta aagtgctgta tgccaaaggg gcgaacgtta ccagtgacaa 1441 aggcattatc gatttcctga atcagtatga agaagcggtc aaagtcgatc cgcgttcgcc 1501 gcaagagatg attgatgaag cggtgcagac ggcgaaacaa tctgatgtgg tggtggctgt 1561 agtcggtgaa gcacagggga tggcgcacga agcctccagc cggaccgata tcactattcc 1621 gcaaagccaa cgtgacttga ttgcggcgct gaaagccacc ggtaaaccgc tggtgctggt 1681 gctgatgaac gggcgtccgc tggcgctggt gaaagaagat cagcaggctg atgcgattct 1741 ggaaacctgg tttgcgggga ctgaaggcgg taatgcaatt gccgatgtat tgtttggcga 1801 ttacaacccg tccggcaagc tgccaatgtc cttcccgcgt tctgtcgggc agatcccggt 1861 gtactacagc catctgaata ccggtcgccc gtataatgcc gacaagccga acaaatacac 1921 ttcgcgttat tttgatgaag ctaacggggc gttgtatccg ttcggctatg ggctgagcta 1981 caccactttc accgtctctg atgtgaaact ttctgcgccg accatgaagc gtgacggcaa 2041 agtgactgcc agcgtgcagg tgacgaacac cggtaagcgc gagggtgcca cggtagtgca 2101 gatgtacttg caggatgtga ctgcttccat gagtcgccct gtgaaacagc tgaaaggctt 2161 tgagaaaatc accctgaaac cgggcgaaac tcagactgtc agcttcccga tcgatattga 2221 ggcgctgaag ttctggaatc aacagatgaa atatgacgcc gagcctggca agttcaatgt 2281 ctttatcggc actgattccg cacgcgttaa gaaaggcgag tttgagttgc tgtaataa //