BBa_K523002 1 BBa_K523002 RBS + bglX (E. coli perisplasmic &#946;-glucosidase) 2011-07-18T11:00:00Z 2015-05-08T01:12:33Z The source ''E. coli'' genome sequence can be seen using GenBank U00096.2 Released HQ 2013 This is the ''E. coli'' beta-glucosidase gene ''bglX''. The part contains the native Ribosome Binding Site. The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site. false false _688_ 0 8799 9 In stock false The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site. The part was created from a lab ''E. coli'' strain using primers: *Forward: ACT AGATCT gcc acg tcg ggc aac **adds BglII site; starts upstream of gene to catch RBS; RFC10 compliant after replacement of <partinfo>BBa_K523000</partinfo> in a normal BioBrick vector. *Reverse: ACT ACTAGT A TTA tta cag caa ctc aaa ctc gcc **adds SpeI site; RFC10 compliant after insertion into vector; has double stop codon. false Sylvia Ispasanie, Mun Ching Lee annotation2123632 1 bglX range2123632 1 38 2338 annotation2123640 1 Predicted (cleaved) signal peptide range2123640 1 38 97 annotation2123630 1 BglII range2123630 1 1 4 annotation2123631 1 Native RBS range2123631 1 22 25 annotation2123633 1 ATG range2123633 1 38 38 annotation2123634 1 TAATAA range2123634 1 2333 2333 annotation2123636 1 G to T silent mutation range2123636 1 2122 2122 annotation2123635 1 PstI site removed range2123635 1 85 85 BBa_K523002_sequence 1 atctgccacgtcgggcaacaaaggaagaaaaatccatatgaaatggctatgttcagtaggaatcgcggtgagtctggccctgcaaccagcactggcggatgatttattcggcaaccatccattaacgcccgaagcgcgggatgcgttcgtcaccgaactgcttaagaaaatgacagttgatgagaaaattggtcagctgcgcttaatcagcgtcggcccggataacccgaaagaggcgatccgcgagatgatcaaagacggtcaggttggggcgattttcaacaccgtaacccgtcaggatatccgcgccatgcaggatcaggtgatggaattaagccgcctgaaaattcctcttttctttgcttacgacgtgctgcacggtcagcgcacggtgttcccgattagcctcggtctggcctcgtcttttaacctcgatgcagtgaaaacggtcggacgtgtctctgcttatgaagcggcagatgatggcctgaatatgacctgggcaccgatggtcgatgtctcgcgcgatccgcgctggggacgtgcttccgaaggttttggcgaagatacgtatctcacctcaacaatgggtaaaaccatggtggaagcgatgcagggtaaaagcccggcagatcgctactcggtgatgaccagcgtcaaacactttgccgcatacggcgcggtagaaggcggtaaagagtacaacaccgtcgatatgagtccgcagcgcctgtttaatgattatatgccgccgtacaaagcggggctggacgcaggcagcggcgcggtgatggtggcgctgaactcgctgaacggcacgccagccacctccgattcctggctgctgaaagatgttctgcgcgaccagtggggctttaaaggcatcaccgtttccgatcacggtgcaatcaaagagctgattaaacatggcacggcggcagacccggaagatgcggtgcgcgtggcgctgaaatccggaatcaacatgagcatgagcgacgagtactactcgaagtatctgcctgggttgattaaatccggcaaagtgacgatggcagagctggacgatgctgcccgccatgtactgaacgttaaatatgatatggggttgtttaacgacccatacagccatttggggccgaaagagtctgacccggtggataccaatgccgaaagccgcctgcaccgtaaagaagcgcgtgaagtggcgcgcgaaagcttggtgttgctgaaaaaccgtctcgaaacgttaccgctgaaaaaatcggccaccattgcggtggttgggccactggcggacagtaaacgtgacgtgatgggcagctggtccgcagccggtgttgccgatcaatccgtgaccgtactgaccgggattaaaaatgccgtcggtgaaaacggtaaagtgctgtatgccaaaggggcgaacgttaccagtgacaaaggcattatcgatttcctgaatcagtatgaagaagcggtcaaagtcgatccgcgttcgccgcaagagatgattgatgaagcggtgcagacggcgaaacaatctgatgtggtggtggctgtagtcggtgaagcacaggggatggcgcacgaagcctccagccggaccgatatcactattccgcaaagccaacgtgacttgattgcggcgctgaaagccaccggtaaaccgctggtgctggtgctgatgaacgggcgtccgctggcgctggtgaaagaagatcagcaggctgatgcgattctggaaacctggtttgcggggactgaaggcggtaatgcaattgccgatgtattgtttggcgattacaacccgtccggcaagctgccaatgtccttcccgcgttctgtcgggcagatcccggtgtactacagccatctgaataccggtcgcccgtataatgccgacaagccgaacaaatacacttcgcgttattttgatgaagctaacggggcgttgtatccgttcggctatgggctgagctacaccactttcaccgtctctgatgtgaaactttctgcgccgaccatgaagcgtgacggcaaagtgactgccagcgtgcaggtgacgaacaccggtaagcgcgagggtgccacggtagtgcagatgtacttgcaggatgtgactgcttccatgagtcgccctgtgaaacagctgaaaggctttgagaaaatcaccctgaaaccgggcgaaactcagactgtcagcttcccgatcgatattgaggcgctgaagttctggaatcaacagatgaaatatgacgccgagcctggcaagttcaatgtctttatcggcactgattccgcacgcgttaagaaaggcgagtttgagttgctgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z