##gff-version 3
##sequence-region BBa_K523002 1 2338
BBa_K523002	.	sequence_feature	38	97	.	+	0	ID=annotation2123640;Name=Predicted (cleaved) signal peptide
BBa_K523002	.	CDS	38	2338	.	+	0	ID=annotation2123632;Name=bglX
BBa_K523002	.	sequence_feature	1	4	.	+	0	ID=annotation2123630;Name=BglII
BBa_K523002	.	sequence_feature	22	25	.	+	0	ID=annotation2123631;Name=Native RBS
BBa_K523002	.	start_codon	38	38	.	+	0	ID=annotation2123633;Name=ATG
BBa_K523002	.	stop_codon	2333	2333	.	+	0	ID=annotation2123634;Name=TAATAA
BBa_K523002	.	point_mutation	2122	2122	.	+	0	ID=annotation2123636;Name=G to T silent mutation
BBa_K523002	.	point_mutation	85	85	.	+	0	ID=annotation2123635;Name=PstI site removed
>BBa_K523002
atctgccacgtcgggcaacaaaggaagaaaaatccatatgaaatggctatgttcagtaggaatcgcggtgagtctggcc
ctgcaaccagcactggcggatgatttattcggcaaccatccattaacgcccgaagcgcgggatgcgttcgtcaccgaac
tgcttaagaaaatgacagttgatgagaaaattggtcagctgcgcttaatcagcgtcggcccggataacccgaaagaggc
gatccgcgagatgatcaaagacggtcaggttggggcgattttcaacaccgtaacccgtcaggatatccgcgccatgcag
gatcaggtgatggaattaagccgcctgaaaattcctcttttctttgcttacgacgtgctgcacggtcagcgcacggtgt
tcccgattagcctcggtctggcctcgtcttttaacctcgatgcagtgaaaacggtcggacgtgtctctgcttatgaagc
ggcagatgatggcctgaatatgacctgggcaccgatggtcgatgtctcgcgcgatccgcgctggggacgtgcttccgaa
ggttttggcgaagatacgtatctcacctcaacaatgggtaaaaccatggtggaagcgatgcagggtaaaagcccggcag
atcgctactcggtgatgaccagcgtcaaacactttgccgcatacggcgcggtagaaggcggtaaagagtacaacaccgt
cgatatgagtccgcagcgcctgtttaatgattatatgccgccgtacaaagcggggctggacgcaggcagcggcgcggtg
atggtggcgctgaactcgctgaacggcacgccagccacctccgattcctggctgctgaaagatgttctgcgcgaccagt
ggggctttaaaggcatcaccgtttccgatcacggtgcaatcaaagagctgattaaacatggcacggcggcagacccgga
agatgcggtgcgcgtggcgctgaaatccggaatcaacatgagcatgagcgacgagtactactcgaagtatctgcctggg
ttgattaaatccggcaaagtgacgatggcagagctggacgatgctgcccgccatgtactgaacgttaaatatgatatgg
ggttgtttaacgacccatacagccatttggggccgaaagagtctgacccggtggataccaatgccgaaagccgcctgca
ccgtaaagaagcgcgtgaagtggcgcgcgaaagcttggtgttgctgaaaaaccgtctcgaaacgttaccgctgaaaaaa
tcggccaccattgcggtggttgggccactggcggacagtaaacgtgacgtgatgggcagctggtccgcagccggtgttg
ccgatcaatccgtgaccgtactgaccgggattaaaaatgccgtcggtgaaaacggtaaagtgctgtatgccaaaggggc
gaacgttaccagtgacaaaggcattatcgatttcctgaatcagtatgaagaagcggtcaaagtcgatccgcgttcgccg
caagagatgattgatgaagcggtgcagacggcgaaacaatctgatgtggtggtggctgtagtcggtgaagcacagggga
tggcgcacgaagcctccagccggaccgatatcactattccgcaaagccaacgtgacttgattgcggcgctgaaagccac
cggtaaaccgctggtgctggtgctgatgaacgggcgtccgctggcgctggtgaaagaagatcagcaggctgatgcgatt
ctggaaacctggtttgcggggactgaaggcggtaatgcaattgccgatgtattgtttggcgattacaacccgtccggca
agctgccaatgtccttcccgcgttctgtcgggcagatcccggtgtactacagccatctgaataccggtcgcccgtataa
tgccgacaagccgaacaaatacacttcgcgttattttgatgaagctaacggggcgttgtatccgttcggctatgggctg
agctacaccactttcaccgtctctgatgtgaaactttctgcgccgaccatgaagcgtgacggcaaagtgactgccagcg
tgcaggtgacgaacaccggtaagcgcgagggtgccacggtagtgcagatgtacttgcaggatgtgactgcttccatgag
tcgccctgtgaaacagctgaaaggctttgagaaaatcaccctgaaaccgggcgaaactcagactgtcagcttcccgatc
gatattgaggcgctgaagttctggaatcaacagatgaaatatgacgccgagcctggcaagttcaatgtctttatcggca
ctgattccgcacgcgttaagaaaggcgagtttgagttgctgtaataa