BBa_K523004
1
BBa_K523004
bglX for BioSandwich
2011-07-28T11:00:00Z
2015-05-08T01:12:33Z
See <partinfo>BBa_K523002</partinfo>
Released HQ 2013
This is a version of <partinfo>BBa_K523002</partinfo> but with no RBS and no stop codon.
There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site.
There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine.
The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for Edinburgh 2011's BioSandwich assembly protocol.
false
false
_688_
0
8799
9
In stock
false
The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
The part was created from a lab ''E. coli'' strain using primers:
* ACT AGATCT atg aaa tgg cta tgt tca gta gg
** Forward; adds BglII site; starts at start codon.
* ACT ACTAGT ACC cag caa ctc aaa ctc gcc ttt c
** Reverse; adds SpeI site; BioSandwich compliant; no stop codons, adds a glycine.
false
Sylvia Ispasanie, Mun Ching Lee
annotation2124177
1
ATG
range2124177
1
5
5
annotation2124176
1
bglX
range2124176
1
5
2299
annotation2124178
1
GG
range2124178
1
2300
2301
annotation2124179
1
BglII
range2124179
1
1
4
annotation2125497
1
PstI site removed
range2125497
1
52
52
BBa_K523004_sequence
1
atctatgaaatggctatgttcagtaggaatcgcggtgagtctggccctgcaaccagcactggcggatgatttattcggcaaccatccattaacgcccgaagcgcgggatgcgttcgtcaccgaactgcttaagaaaatgacagttgatgagaaaattggtcagctgcgcttaatcagcgtcggcccggataacccgaaagaggcgatccgcgagatgatcaaagacggtcaggttggggcgattttcaacaccgtaacccgtcaggatatccgcgccatgcaggatcaggtgatggaattaagccgcctgaaaattcctcttttctttgcttacgacgtgctgcacggtcagcgcacggtgttcccgattagcctcggtctggcctcgtcttttaacctcgatgcagtgaaaacggtcggacgtgtctctgcttatgaagcggcagatgatggcctgaatatgacctgggcaccgatggtcgatgtctcgcgcgatccgcgctggggacgtgcttccgaaggttttggcgaagatacgtatctcacctcaacaatgggtaaaaccatggtggaagcgatgcagggtaaaagcccggcagatcgctactcggtgatgaccagcgtcaaacactttgccgcatacggcgcggtagaaggcggtaaagagtacaacaccgtcgatatgagtccgcagcgcctgtttaatgattatatgccgccgtacaaagcggggctggacgcaggcagcggcgcggtgatggtggcgctgaactcgctgaacggcacgccagccacctccgattcctggctgctgaaagatgttctgcgcgaccagtggggctttaaaggcatcaccgtttccgatcacggtgcaatcaaagagctgattaaacatggcacggcggcagacccggaagatgcggtgcgcgtggcgctgaaatccggaatcaacatgagcatgagcgacgagtactactcgaagtatctgcctgggttgattaaatccggcaaagtgacgatggcagagctggacgatgctgcccgccatgtactgaacgttaaatatgatatggggttgtttaacgacccatacagccatttggggccgaaagagtctgacccggtggataccaatgccgaaagccgcctgcaccgtaaagaagcgcgtgaagtggcgcgcgaaagcttggtgttgctgaaaaaccgtctcgaaacgttaccgctgaaaaaatcggccaccattgcggtggttgggccactggcggacagtaaacgtgacgtgatgggcagctggtccgcagccggtgttgccgatcaatccgtgaccgtactgaccgggattaaaaatgccgtcggtgaaaacggtaaagtgctgtatgccaaaggggcgaacgttaccagtgacaaaggcattatcgatttcctgaatcagtatgaagaagcggtcaaagtcgatccgcgttcgccgcaagagatgattgatgaagcggtgcagacggcgaaacaatctgatgtggtggtggctgtagtcggtgaagcacaggggatggcgcacgaagcctccagccggaccgatatcactattccgcaaagccaacgtgacttgattgcggcgctgaaagccaccggtaaaccgctggtgctggtgctgatgaacgggcgtccgctggcgctggtgaaagaagatcagcaggctgatgcgattctggaaacctggtttgcggggactgaaggcggtaatgcaattgccgatgtattgtttggcgattacaacccgtccggcaagctgccaatgtccttcccgcgttctgtcgggcagatcccggtgtactacagccatctgaataccggtcgcccgtataatgccgacaagccgaacaaatacacttcgcgttattttgatgaagctaacggggcgttgtatccgttcggctatgggctgagctacaccactttcaccgtctctgatgtgaaactttctgcgccgaccatgaagcgtgacggcaaagtgactgccagcgtgcaggtgacgaacaccggtaagcgcgagggtgccacggtagtgcagatgtacttgcaggatgtgactgcttccatgagtcgccctgtgaaacagctgaaaggctttgagaaaatcaccctgaaaccgggcgaaactcagactgtcagcttcccgatcgatattgaggcgctgaagttctggaatcaacagatgaaatatgacgccgagcctggcaagttcaatgtctttatcggcactgattccgcacgcgttaagaaaggcgagtttgagttgctggg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z