LOCUS       BBa_K523006             2675 bp    DNA     linear       17-May-2024
DEFINITION  Plac + LacZ + malS
ACCESSION   BBa_K523006
VERSION     BBa_K523006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    609..612
                     /label=BglII
     RBS             628..631
                     /label=Native RBS
     misc_feature    642..642
                     /label=ATG
     CDS             642..2675
                     /label=malS
     misc_feature    2670..2670
                     /label=TAATAA
     misc_feature    642..692
                     /label=Predicted (cleaved) signal peptide
     misc_feature    609..2675
                     /label=BBa_K523001
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagat ctgaaatcgc agcaataagg actcatccgc catgaaactc gccgcctgtt
      661 ttctgacact ccttcctggc ttcgccgttg ccgccagctg gacttctccg gggtttcccg
      721 cctttagcga acaggggaca ggaacatttg tcagccacgc gcagttgccc aaaggtacgc
      781 gtccactaac gctaaatttt gaccaacagt gctggcagcc tgcggatgcg ataaaactca
      841 atcagatgct ttccctgcaa ccttgtagca acacgccgcc tcaatggcga ttgttcaggg
      901 acggcgaata tacgctgcaa atagacaccc gctccggtac gccaacattg atgatttcca
      961 tccagaacgc cgccgaaccg gtagcaagcc tggtccgtga atgcccgaaa tgggatggat
     1021 taccgctcac agtggatgtc agcgccactt tcccggaagg agccgccgta cgggattatt
     1081 acagccagca aattgcgata gtgaagaacg gtcaaataat gttacaaccc gctgccacca
     1141 gcaacggttt actcctgctg gaacgggcag aaactgacac atccgcccct ttcgactggc
     1201 ataacgccac ggtttacttt gtgctgacag atcgtttcga aaacggcgat cccagtaatg
     1261 accagagtta cggacgtcat aaagacggta tggcggaaat tggcactttt cacggcggcg
     1321 atttacgcgg cctgaccaac aaactggatt acctccagca gttgggcgtt aatgctttat
     1381 ggataagcgc cccatttgag caaattcacg gctgggtcgg cggcggtaca aaaggcgatt
     1441 tcccgcatta tgcctaccac ggttattaca cacaggactg gacgaatctt gatgccaata
     1501 tgggcaacga agccgatcta cggacgctgg ttgatagcgc acatcagcgc ggtattcgta
     1561 ttctctttga tgtcgtgatg aaccacaccg gctatgccac gctggcggat atgcaggagt
     1621 atcagtttgg cgcgttatat ctttctggtg acgaagtgaa aaaatcgctg ggtgaacgct
     1681 ggagcgactg gaaacctgcc gccgggcaaa cctggcatag ctttaacgat tacattaatt
     1741 tcagcgacaa aacaggctgg gataaatggt ggggaaaaaa ctggatcaga acggatatcg
     1801 gcgattacga caatcctgga ttcgacgatc tcactatgtc gctagccttt ttgccggata
     1861 tcaaaaccga atcaactacc gcttctggtc tgccggtgtt ctataaaaac aaaatggata
     1921 cccacgccaa agccattgac ggctatacgc cgcgcgatta cttaacccac tggttaagtc
     1981 agtgggtccg cgactatggg attgatggtt ttcgggtcga taccgccaaa catgttgagt
     2041 tgcccgcctg gcagcaactg aaaaccgaag ccagcgccgc gcttcgcgaa tggaaaaaag
     2101 ctaaccccga caaagcatta gatgacaaac ctttctggat gaccggtgaa gcctggggcc
     2161 acggcgtgat gcaaagtgac tactatcgcc acggcttcga tgcgatgatc aatttcgatt
     2221 atcaggagca ggcggcgaaa gcagtcgact gtctggcgca gatggatacg acctggcagc
     2281 aaatggcgga gaaattgcag ggtttcaacg tgttgagcta cctctcgtcg catgataccc
     2341 gcctgttccg tgaagggggc gacaaagcag cagagttatt actattagcg ccaggcgcgg
     2401 tacaaatctt ttatggtgat gaatcctcgc gtccgttcgg tcctacaggt tctgatccgc
     2461 tgcaaggtac acgttcggat atgaactggc aggatgttag cggtaaatct gccgccagcg
     2521 tcgcgcactg gcagaaaatc agccagttcc gcgcccgcca tcccgcaatt ggcgcgggca
     2581 aacaaacgac acttttgctg aagcagggct acggctttgt tcgtgagcat ggcgacgata
     2641 aagtgctggt cgtctgggca gggcaacagt aataa
//