LOCUS       BBa_K523007             2660 bp    DNA     linear       27-Dec-2024
DEFINITION  Plac + LacZ + malS (dubious version)
ACCESSION   BBa_K523007
VERSION     BBa_K523007.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    609..611
                     /label=SacI
     RBS             612..618
                     /label=rbs
     RBS             609..618
                     /label=BBa_J15001
     promoter        1..618
                     /label=BBa_K523005
     CDS             631..2658
                     /label=malS
     misc_feature    2659..2660
                     /label=GG
     misc_feature    627..630
                     /label=BglII
     misc_feature    631..631
                     /label=ATG
     CDS             627..2660
                     /label=BBa_K523003
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagct caaggaggta ctagagatct atgaaactcg ccgcctgttt tctgacactc
      661 cttcctggct tcgccgttgc cgccagctgg acttctccgg ggtttcccgc ctttagcgaa
      721 caggggacag gaacatttgt cagccacgcg cagttgccca aaggtacgcg tccactaacg
      781 ctaaattttg accaacagtg ctggcagcct gcggatgcga taaaactcaa tcagatgctt
      841 tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcaggga cggcgaatat
      901 acgctgcaaa tagacacccg ctccggtacg ccaacattga tgatttccat ccagaacgcc
      961 gccgaaccgg tagcaagcct ggtccgtgaa tgcccgaaat gggatggatt accgctcaca
     1021 gtggatgtca gcgccacttt cccggaagga gccgccgtac gggattatta cagccagcaa
     1081 attgcgatag tgaagaacgg tcaaataatg ttacaacccg ctgccaccag caacggttta
     1141 ctcctgctgg aacgggcaga aactgacaca tccgcccctt tcgactggca taacgccacg
     1201 gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga ccagagttac
     1261 ggacgtcata aagacggtat ggcggaaatt ggcacttttc acggcggcga tttacgcggc
     1321 ctgaccaaca aactggatta cctccagcag ttgggcgtta atgctttatg gataagcgcc
     1381 ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggcgattt cccgcattat
     1441 gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat gggcaacgaa
     1501 gccgatctac ggacgctggt tgatagcgca catcagcgcg gtattcgtat tctctttgat
     1561 gtcgtgatga accacaccgg ctatgccacg ctggcggata tgcaggagta tcagtttggc
     1621 gcgttatatc tttctggtga cgaagtgaaa aaatcgctgg gtgaacgctg gagcgactgg
     1681 aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt cagcgacaaa
     1741 acaggctggg ataaatggtg gggaaaaaac tggatcagaa cggatatcgg cgattacgac
     1801 aatcctggat tcgacgatct cactatgtcg ctagcctttt tgccggatat caaaaccgaa
     1861 tcaactaccg cttctggtct gccggtgttc tataaaaaca aaatggatac ccacgccaaa
     1921 gccattgacg gctatacgcc gcgcgattac ttaacccact ggttaagtca gtgggtccgc
     1981 gactatggga ttgatggttt tcgggtcgat accgccaaac atgttgagtt gcccgcctgg
     2041 cagcaactga aaaccgaagc cagcgccgcg cttcgcgaat ggaaaaaagc taaccccgac
     2101 aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca cggcgtgatg
     2161 caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta tcaggagcag
     2221 gcggcgaaag cagtcgactg tctggcgcag atggatacga cctggcagca aatggcggag
     2281 aaattgcagg gtttcaacgt gttgagctac ctctcgtcgc atgatacccg cctgttccgt
     2341 gaagggggcg acaaagcagc agagttatta ctattagcgc caggcgcggt acaaatcttt
     2401 tatggtgatg aatcctcgcg tccgttcggt cctacaggtt ctgatccgct gcaaggtaca
     2461 cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccagcgt cgcgcactgg
     2521 cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa acaaacgaca
     2581 cttttgctga agcagggcta cggctttgtt cgtgagcatg gcgacgataa agtgctggtc
     2641 gtctgggcag ggcaacaggg
//