LOCUS BBa_K523014 2946 bp DNA linear 22-Dec-2024 DEFINITION Plac + LacZ + bglX ACCESSION BBa_K523014 VERSION BBa_K523014.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=SacI misc_feature 248..285 /label=CAP binding site misc_feature 332..352 /label=LacI binding site RBS 359..362 /label=rbs CDS 370..600 /label=lacZ' promoter 320..325 /label=-10 promoter 297..302 /label=-35 misc_feature 1..600 /label=BBa_J33207 misc_feature 609..612 /label=BglII misc_feature 630..633 /label=Native RBS CDS 646..2946 /label=bglX misc_feature 646..646 /label=ATG misc_feature 2941..2941 /label=TAATAA misc_feature 693..693 /label=PstI site removed misc_feature 2730..2730 /label=G to T silent mutation misc_feature 646..705 /label=Predicted (cleaved) signal peptide misc_feature 609..2946 /label=BBa_K523002 ORIGIN 1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac 61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt 121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc 181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag 361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg 421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga 601 tactagagat ctgccacgtc gggcaacaaa ggaagaaaaa tccatatgaa atggctatgt 661 tcagtaggaa tcgcggtgag tctggccctg caaccagcac tggcggatga tttattcggc 721 aaccatccat taacgcccga agcgcgggat gcgttcgtca ccgaactgct taagaaaatg 781 acagttgatg agaaaattgg tcagctgcgc ttaatcagcg tcggcccgga taacccgaaa 841 gaggcgatcc gcgagatgat caaagacggt caggttgggg cgattttcaa caccgtaacc 901 cgtcaggata tccgcgccat gcaggatcag gtgatggaat taagccgcct gaaaattcct 961 cttttctttg cttacgacgt gctgcacggt cagcgcacgg tgttcccgat tagcctcggt 1021 ctggcctcgt cttttaacct cgatgcagtg aaaacggtcg gacgtgtctc tgcttatgaa 1081 gcggcagatg atggcctgaa tatgacctgg gcaccgatgg tcgatgtctc gcgcgatccg 1141 cgctggggac gtgcttccga aggttttggc gaagatacgt atctcacctc aacaatgggt 1201 aaaaccatgg tggaagcgat gcagggtaaa agcccggcag atcgctactc ggtgatgacc 1261 agcgtcaaac actttgccgc atacggcgcg gtagaaggcg gtaaagagta caacaccgtc 1321 gatatgagtc cgcagcgcct gtttaatgat tatatgccgc cgtacaaagc ggggctggac 1381 gcaggcagcg gcgcggtgat ggtggcgctg aactcgctga acggcacgcc agccacctcc 1441 gattcctggc tgctgaaaga tgttctgcgc gaccagtggg gctttaaagg catcaccgtt 1501 tccgatcacg gtgcaatcaa agagctgatt aaacatggca cggcggcaga cccggaagat 1561 gcggtgcgcg tggcgctgaa atccggaatc aacatgagca tgagcgacga gtactactcg 1621 aagtatctgc ctgggttgat taaatccggc aaagtgacga tggcagagct ggacgatgct 1681 gcccgccatg tactgaacgt taaatatgat atggggttgt ttaacgaccc atacagccat 1741 ttggggccga aagagtctga cccggtggat accaatgccg aaagccgcct gcaccgtaaa 1801 gaagcgcgtg aagtggcgcg cgaaagcttg gtgttgctga aaaaccgtct cgaaacgtta 1861 ccgctgaaaa aatcggccac cattgcggtg gttgggccac tggcggacag taaacgtgac 1921 gtgatgggca gctggtccgc agccggtgtt gccgatcaat ccgtgaccgt actgaccggg 1981 attaaaaatg ccgtcggtga aaacggtaaa gtgctgtatg ccaaaggggc gaacgttacc 2041 agtgacaaag gcattatcga tttcctgaat cagtatgaag aagcggtcaa agtcgatccg 2101 cgttcgccgc aagagatgat tgatgaagcg gtgcagacgg cgaaacaatc tgatgtggtg 2161 gtggctgtag tcggtgaagc acaggggatg gcgcacgaag cctccagccg gaccgatatc 2221 actattccgc aaagccaacg tgacttgatt gcggcgctga aagccaccgg taaaccgctg 2281 gtgctggtgc tgatgaacgg gcgtccgctg gcgctggtga aagaagatca gcaggctgat 2341 gcgattctgg aaacctggtt tgcggggact gaaggcggta atgcaattgc cgatgtattg 2401 tttggcgatt acaacccgtc cggcaagctg ccaatgtcct tcccgcgttc tgtcgggcag 2461 atcccggtgt actacagcca tctgaatacc ggtcgcccgt ataatgccga caagccgaac 2521 aaatacactt cgcgttattt tgatgaagct aacggggcgt tgtatccgtt cggctatggg 2581 ctgagctaca ccactttcac cgtctctgat gtgaaacttt ctgcgccgac catgaagcgt 2641 gacggcaaag tgactgccag cgtgcaggtg acgaacaccg gtaagcgcga gggtgccacg 2701 gtagtgcaga tgtacttgca ggatgtgact gcttccatga gtcgccctgt gaaacagctg 2761 aaaggctttg agaaaatcac cctgaaaccg ggcgaaactc agactgtcag cttcccgatc 2821 gatattgagg cgctgaagtt ctggaatcaa cagatgaaat atgacgccga gcctggcaag 2881 ttcaatgtct ttatcggcac tgattccgca cgcgttaaga aaggcgagtt tgagttgctg 2941 taataa //