##gff-version 3
##sequence-region BBa_K523014 1 2946
BBa_K523014	.	engineered_region	1	600	.	+	0	ID=BBa_J33207;Name=BBa_J33207
BBa_K523014	.	promoter	297	302	.	+	0	ID=annotation1907860;Name=-35;Parent=BBa_J33207
BBa_K523014	.	CDS	370	600	.	+	0	ID=annotation1907858;Name=lacZ';Parent=BBa_J33207
BBa_K523014	.	promoter	320	325	.	+	0	ID=annotation1907859;Name=-10;Parent=BBa_J33207
BBa_K523014	.	operator	248	285	.	+	0	ID=annotation1907855;Name=CAP binding site;Parent=BBa_J33207
BBa_K523014	.	operator	332	352	.	+	0	ID=annotation1907856;Name=LacI binding site;Parent=BBa_J33207
BBa_K523014	.	sequence_feature	1	3	.	+	0	ID=annotation1907854;Name=SacI;Parent=BBa_J33207
BBa_K523014	.	ribosome_entry_site	359	362	.	+	0	ID=annotation1907857;Name=rbs;Parent=BBa_J33207
BBa_K523014	.	engineered_region	609	2946	.	+	0	ID=BBa_K523002;Name=BBa_K523002
BBa_K523014	.	sequence_feature	630	633	.	+	0	ID=annotation2123631;Name=Native RBS;Parent=BBa_K523002
BBa_K523014	.	sequence_feature	646	705	.	+	0	ID=annotation2123640;Name=Predicted (cleaved) signal peptide;Parent=BBa_K523002
BBa_K523014	.	CDS	646	2946	.	+	0	ID=annotation2123632;Name=bglX;Parent=BBa_K523002
BBa_K523014	.	start_codon	646	646	.	+	0	ID=annotation2123633;Name=ATG;Parent=BBa_K523002
BBa_K523014	.	sequence_feature	609	612	.	+	0	ID=annotation2123630;Name=BglII;Parent=BBa_K523002
BBa_K523014	.	stop_codon	2941	2941	.	+	0	ID=annotation2123634;Name=TAATAA;Parent=BBa_K523002
BBa_K523014	.	point_mutation	2730	2730	.	+	0	ID=annotation2123636;Name=G to T silent mutation;Parent=BBa_K523002
BBa_K523014	.	point_mutation	693	693	.	+	0	ID=annotation2123635;Name=PstI site removed;Parent=BBa_K523002
>BBa_K523014
ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctg
caactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgc
ccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag
cgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggc
tcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggc
cgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgcc
agctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttg
cctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagagatctgccacgtcgggcaacaaagg
aagaaaaatccatatgaaatggctatgttcagtaggaatcgcggtgagtctggccctgcaaccagcactggcggatgat
ttattcggcaaccatccattaacgcccgaagcgcgggatgcgttcgtcaccgaactgcttaagaaaatgacagttgatg
agaaaattggtcagctgcgcttaatcagcgtcggcccggataacccgaaagaggcgatccgcgagatgatcaaagacgg
tcaggttggggcgattttcaacaccgtaacccgtcaggatatccgcgccatgcaggatcaggtgatggaattaagccgc
ctgaaaattcctcttttctttgcttacgacgtgctgcacggtcagcgcacggtgttcccgattagcctcggtctggcct
cgtcttttaacctcgatgcagtgaaaacggtcggacgtgtctctgcttatgaagcggcagatgatggcctgaatatgac
ctgggcaccgatggtcgatgtctcgcgcgatccgcgctggggacgtgcttccgaaggttttggcgaagatacgtatctc
acctcaacaatgggtaaaaccatggtggaagcgatgcagggtaaaagcccggcagatcgctactcggtgatgaccagcg
tcaaacactttgccgcatacggcgcggtagaaggcggtaaagagtacaacaccgtcgatatgagtccgcagcgcctgtt
taatgattatatgccgccgtacaaagcggggctggacgcaggcagcggcgcggtgatggtggcgctgaactcgctgaac
ggcacgccagccacctccgattcctggctgctgaaagatgttctgcgcgaccagtggggctttaaaggcatcaccgttt
ccgatcacggtgcaatcaaagagctgattaaacatggcacggcggcagacccggaagatgcggtgcgcgtggcgctgaa
atccggaatcaacatgagcatgagcgacgagtactactcgaagtatctgcctgggttgattaaatccggcaaagtgacg
atggcagagctggacgatgctgcccgccatgtactgaacgttaaatatgatatggggttgtttaacgacccatacagcc
atttggggccgaaagagtctgacccggtggataccaatgccgaaagccgcctgcaccgtaaagaagcgcgtgaagtggc
gcgcgaaagcttggtgttgctgaaaaaccgtctcgaaacgttaccgctgaaaaaatcggccaccattgcggtggttggg
ccactggcggacagtaaacgtgacgtgatgggcagctggtccgcagccggtgttgccgatcaatccgtgaccgtactga
ccgggattaaaaatgccgtcggtgaaaacggtaaagtgctgtatgccaaaggggcgaacgttaccagtgacaaaggcat
tatcgatttcctgaatcagtatgaagaagcggtcaaagtcgatccgcgttcgccgcaagagatgattgatgaagcggtg
cagacggcgaaacaatctgatgtggtggtggctgtagtcggtgaagcacaggggatggcgcacgaagcctccagccgga
ccgatatcactattccgcaaagccaacgtgacttgattgcggcgctgaaagccaccggtaaaccgctggtgctggtgct
gatgaacgggcgtccgctggcgctggtgaaagaagatcagcaggctgatgcgattctggaaacctggtttgcggggact
gaaggcggtaatgcaattgccgatgtattgtttggcgattacaacccgtccggcaagctgccaatgtccttcccgcgtt
ctgtcgggcagatcccggtgtactacagccatctgaataccggtcgcccgtataatgccgacaagccgaacaaatacac
ttcgcgttattttgatgaagctaacggggcgttgtatccgttcggctatgggctgagctacaccactttcaccgtctct
gatgtgaaactttctgcgccgaccatgaagcgtgacggcaaagtgactgccagcgtgcaggtgacgaacaccggtaagc
gcgagggtgccacggtagtgcagatgtacttgcaggatgtgactgcttccatgagtcgccctgtgaaacagctgaaagg
ctttgagaaaatcaccctgaaaccgggcgaaactcagactgtcagcttcccgatcgatattgaggcgctgaagttctgg
aatcaacagatgaaatatgacgccgagcctggcaagttcaatgtctttatcggcactgattccgcacgcgttaagaaag
gcgagtttgagttgctgtaataa