LOCUS       BBa_K523015             1977 bp    DNA     linear       14-May-2024
DEFINITION  Plac + RBS + cenA (endoglucanase)
ACCESSION   BBa_K523015
VERSION     BBa_K523015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    609..611
                     /label=SacI
     RBS             612..618
                     /label=rbs
     RBS             609..618
                     /label=BBa_J15001
     CDS             625..1974
                     /label=cenA coding sequence
     CDS             625..1977
                     /label=BBa_K118023
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagct caaggaggta ctagatgtcc acccgcagaa ccgccgcagc gctgctggcg
      661 gccgcggccg tcgccgtcgg cggtctgacc gccctcacca ccaccgccgc gcaggcggct
      721 cccggctgcc gcgtcgacta cgccgtcacc aaccagtggc ccggcggctt cggcgccaac
      781 gtcacgatca ccaacctcgg cgaccccgtc tcgtcgtgga agctcgactg gacctacacc
      841 gcaggccagc ggatccagca gctgtggaac ggcaccgcgt cgaccaacgg cggccaggtc
      901 tccgtcacca gcctgccctg gaacggcagc atcccgaccg gcggcacggc gtcgttcggg
      961 ttcaacggct cgtgggccgg gtccaacccg acgccggcgt cgttctcgct caacggcacc
     1021 acctgcacgg gcaccgtgcc gacgaccagc cccacgccga ccccgacgcc gacgaccccc
     1081 acgccgacgc cgaccccgac ccccaccccc acgccgacgg tcacgccgca gccgaccagc
     1141 ggcttctacg tcgacccgac gacgcagggc taccgcgcgt ggcaggccgc gtccggcacg
     1201 gacaaggcgc tgctcgagaa gatcgcgctc accccgcagg cgtactgggt cggcaactgg
     1261 gccgacgcgt cgcacgcgca ggccgaggtc gccgactaca ccggccgcgc cgtcgcggcc
     1321 gggaagacgc cgatgctcgt cgtctacgcg atcccgggcc gcgactgcgg ctcgcactcc
     1381 ggcggtggtg tgtccgagtc cgagtacgcg cgctgggtcg acaccgtcgc gcagggcatc
     1441 aagggcaacc cgatcgtgat cctcgagccc gacgcgctcg cgcagctcgg cgactgctcc
     1501 ggccagggtg accgcgtcgg cttcctcaag tacgccgcca agtcgctcac cctcaagggc
     1561 gcgcgcgtct acatcgacgc gggccacgcg aagtggctgt cggtcgacac gccggtgaac
     1621 cgcctcaacc aggtcggctt cgagtacgcg gtgggcttcg cgctcaacac gtcgaactac
     1681 cagacgacgg cggacagcaa ggcgtacggc cagcagatct cgcagcggct gggcggcaag
     1741 aagttcgtca tcgacacctc gcgcaacggc aacggctcga acggcgagtg gtgcaacccg
     1801 cgcggccgcg cgctcggcga acgcccggtc gcggtgaacg acggctccgg cctggacgcg
     1861 ctcctgtggg tcaagctgcc cggcgagtcc gacggcgcgt gcaacggcgg cccggccgcc
     1921 ggccagtggt ggcaggagat cgccctggag atggcgcgca acgccaggtg gtaataa
//