##gff-version 3
##sequence-region BBa_K523016 1 2085
BBa_K523016	.	engineered_region	1	600	.	+	0	ID=BBa_J33207;Name=BBa_J33207
BBa_K523016	.	CDS	370	600	.	+	0	ID=annotation1907858;Name=lacZ';Parent=BBa_J33207
BBa_K523016	.	promoter	297	302	.	+	0	ID=annotation1907860;Name=-35;Parent=BBa_J33207
BBa_K523016	.	ribosome_entry_site	359	362	.	+	0	ID=annotation1907857;Name=rbs;Parent=BBa_J33207
BBa_K523016	.	operator	332	352	.	+	0	ID=annotation1907856;Name=LacI binding site;Parent=BBa_J33207
BBa_K523016	.	promoter	320	325	.	+	0	ID=annotation1907859;Name=-10;Parent=BBa_J33207
BBa_K523016	.	sequence_feature	1	3	.	+	0	ID=annotation1907854;Name=SacI;Parent=BBa_J33207
BBa_K523016	.	operator	248	285	.	+	0	ID=annotation1907855;Name=CAP binding site;Parent=BBa_J33207
BBa_K523016	.	CDS	625	2085	.	+	0	ID=BBa_K118022;Name=BBa_K118022
BBa_K523016	.	CDS	625	2082	.	+	0	ID=annotation1996993;Name=cex coding sequence;Parent=BBa_K118022
BBa_K523016	.	point_mutation	1405	1410	.	+	0	ID=annotation1996994;Name=PstI site removed;Parent=BBa_K118022
BBa_K523016	.	ribosome_entry_site	609	618	.	+	0	ID=BBa_J15001;Name=BBa_J15001
BBa_K523016	.	sequence_feature	609	611	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K523016	.	ribosome_entry_site	612	618	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
>BBa_K523016
ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctg
caactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgc
ccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag
cgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggc
tcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggc
cgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgcc
agctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttg
cctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagagctcaaggaggtactagatgcctag
gaccacgcccgcacccggccacccggcccgcggcgcccgcaccgctctgcgcacgacgctcgccgccgcggcggcgacg
ctcgtcgtcggcgccacggtcgtgctgcccgcccaggccgcgaccacgctcaaggaggccgccgacggcgccggccggg
acttcggcttcgcgctcgaccccaaccggctctcggaggcgcagtacaaggcgatcgccgacagcgagttcaacctcgt
cgtcgccgagaacgcgatgaagtgggacgccaccgagccctcgcagaacagcttctccttcggcgcgggcgaccgcgtc
gcgagctacgccgccgacaccggcaaggagctgtacggccacacgctcgtatggcactcgcagctgcccgactgggcga
agaacctcaacggctccgcgttcgagagcgcgatggtcaaccacgtgacgaaggtcgccgaccacttcgagggcaaggt
cgcgtcgtgggacgtcgtcaacgaggcgttcgccgacggcggcggccgccggcaggactcggcgttccagcagaagctc
ggcaacggctacatcgagaccgcgttccgggcggcacgtgcggcggacccgaccgccaagctgtgcatcaacgactaca
acgtcgagggcatcaacgcgaagagcaactcgctctacgacctcgtcaaggacttcaaggcgcgcggcgtcccgctcga
ctgcgtcgggttccagtcgcacctcatcgtcggccaggtgccgggcgacttccggcagaacctgcaacggttcgcggac
ctgggcgtggacgtgcgcatcaccgagctcgacatccgcatgcggacgccctccgacgcgaccaagctcgcgacccagg
cggccgactacaagaaggtcgtgcaggcctgcatgcaggtgacccgctgccagggcgtgaccgtctggggcatcaccga
caagtactcgtgggtgccggacgtcttcccgggcgagggggccgcgctggtgtgggacgcgagctacgccaagaagccg
gcctacgccgccgtgatggaggccttcggcgcgagcccgacgccgacgcccaccacgccgaccccgacgcccacgacgc
cgacgccgaccccgacgtccggtccggccgggtgccaggtgctgtggggcgtcaaccagtggaacaccggcttcaccgc
gaacgtcaccgtgaagaacacgtcctccgctccggtcgacggctggacgctcacgttcagcttcccgtccggccagcag
gtcacccaggcgtggagctcgacggtcacgcagtccggctcggccgtgacggtccgcaacgccccgtggaacggctcga
tcccggcgggcggcaccgcgcagttcggcttcaacggctcgcacacgggcaccaacgccgcgccgacggcgttctcgct
caacggcacgccctgcacggtcggctaataa