LOCUS       BBa_K523019             3047 bp    DNA     linear       22-Nov-2024
DEFINITION  Plac + INP-cex
ACCESSION   BBa_K523019
VERSION     BBa_K523019.1  
FEATURES             Location/Qualifiers
     misc_feature    1..32
                     /label=Spacer
     misc_feature    38..589
                     /label=Plac - LacZ
     CDS             359..589
                     /label=LacZ
     misc_feature    597..625
                     /label=Spacer
     RBS             611..614
                     /label=rbs
     CDS             623..2958
                     /label=INP-cex fusion
     misc_feature    2951..2951
                     /label=TAA
     misc_feature    2942..2969
                     /label=Spacer
     misc_feature    623..623
                     /label=ATG
     promoter        286..291
                     /label=-35
     promoter        309..314
                     /label=-10
     RBS             348..351
                     /label=rbs
     misc_feature    1565..1594
                     /label=Spacer (Linker)
ORIGIN
        1 ttaatacgac tcactatagg gagaggaggt acgatctgct ggggcaaacc agcgtggacc
       61 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
      121 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
      181 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      241 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      301 ccggctcgta tgttgtgtga aattgtgagc ggataacaat ttcacacagg aaacagctat
      361 gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      421 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      481 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
      541 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgat actagctgat
      601 atttcgcgta aggaaatcca ttatgggatc tatgaccctg gataaagcgc tggtgctgcg
      661 cacttgtgcc aataatatgg ccgaccattg tggcctgatc tggcctgctt ctggtacagt
      721 tgaaagccgc tattggcagt ctacacgtcg tcacgaaaac gggctggttg ggctgctgtg
      781 gggtgccgga acatccgcct ttctgagcgt tcatgccgat gctcgttgga ttgtttgtga
      841 ggttgctgtt gccgacatca ttagcctgga agaaccgggt atggtgaaat ttcctcgtgc
      901 tgaagtggtc catgttggtg atcgtattag cgcctcccat ttcatttcag ctcgtcaggc
      961 tgatccagca agcacatcaa caagcacctc taccagtaca ctgacaccaa tgccaaccgc
     1021 tatccctact cctatgccgg ctgtagcctc tgttactctg cctgtggccg aacaagctcg
     1081 ccacgaagtc tttgacgttg cctctgtgtc tgctgctgct gctcctgtta acactctgcc
     1141 tgttaccacg cctcaaaatc tgcaaacggc gacctatggc tcaacactga gtggtgataa
     1201 ccattctcgt ctgattgccg ggtatggctc taacgaaacc gccggaaacc attctgatct
     1261 gatcggtggt cacgattgta cgctgatggc aggtgatcaa tctcgtctga ccgctggcaa
     1321 aaattccgtt ctgaccgctg gggcacgttc taaactgatc gggagcgaag gctctactct
     1381 gtccgccgga gaagattcta ccctgatctt tcgtctgtgg gatggtaaac gttatcgtca
     1441 gctggtcgct cgtacaggtg aaaatggtgt ggaagccgac attccgtatt atgtgaatga
     1501 ggacgatgat attgtggaca aaccggacga agatgatgat tggatcgaag tgaaaggtac
     1561 tagcccgacg accagcccca cgccgacccc gacgggatct atggcgacca cgctcaagga
     1621 ggccgccgac ggcgccggcc gggacttcgg cttcgcgctc gaccccaacc ggctctcgga
     1681 ggcgcagtac aaggcgatcg ccgacagcga gttcaacctc gtcgtcgccg agaacgcgat
     1741 gaagtggggc gccaccgagc cctcgcagaa cagcttctcc ttcggcgcgg gcgaccgcgt
     1801 cgcgagctac gccgccgaca ccggcaagga gctgtacggc cacacgctcg tctggcactc
     1861 gcagctgccc gactgggcga agaacctcaa cggctccgcg ttcgagagcg cgatggtcaa
     1921 ccacgtgacg aaggtcgccg accacttcga gggcaaggtc gcgtcgtggg acgtcgtcaa
     1981 cgaggcgttc gccgacggcg gcggccgccg gcaggactcg gcgttccagc agaagctcgg
     2041 caacggctac atcgagaccg cgttccgggc ggcacgtgcg gcggacccga ccgccaagct
     2101 gtgcatcaac gactacaacg tcgagggcat caacgcgaag agcaactcgc tctacgacct
     2161 cgtcaaggac ttcaaggcgc gcggcgtccc gctcgactgc gtcgggttcc agtcgcacct
     2221 catcgtcggc caggtgccgg gcgacttccg gcagaacctg caacggttcg cggacctggg
     2281 cgtggacgtg cgcatcaccg agctcgacat ccgcatgcgg acgccctccg acgcgaccaa
     2341 gctcgcgacc caggcggccg actacaagaa ggtcgtgcag gcctgcatgc aggtgacccg
     2401 ctgccagggc gtgaccgtct ggggcatcac cgacaagtac tcgtgggtgc cggacgtctt
     2461 cccgggcgag ggggccgcgc tggtgtggga cgcgagctac gccaagaagc cggcctacgc
     2521 cgccgtgatg gaggccttcg gcgcgagccc gacgccgacg cccaccacgc cgaccccgac
     2581 gcccacgacg ccgacgccga ccccgacgtc cggtccggcc gggtgccagg tgctgtgggg
     2641 cgtcaaccag tggaacaccg gcttcaccgc gaacgtcacc gtgaagaaca cgtcctccgc
     2701 tccggtcgac ggctggacgc tcacgttcag cttcccgtcc ggccagcagg tcacccaggc
     2761 gtggagctcg acggtcacgc agtccggctc ggccgtgacg gtccgcaacg ccccgtggaa
     2821 cggctcgatc ccggcgggcg gcaccgcgca gttcggcttc aacggctcgc acacgggcac
     2881 caacgccgcg ccgacggcgt tctcgctcaa cggcacgccc tgcacggtcg gctctactag
     2941 caacatatca taacggagtg atcgcaatgg gatctgttgt ttgtcggtga acgctctcta
     3001 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//