LOCUS BBa_K523020 4016 bp DNA linear 05-Nov-2024 DEFINITION Plac + INP-bglX ACCESSION BBa_K523020 VERSION BBa_K523020.1 FEATURES Location/Qualifiers misc_feature 1..32 /label=Spacer misc_feature 38..589 /label=Plac - LacZ CDS 359..589 /label=LacZ misc_feature 597..625 /label=Spacer CDS 623..3922 /label=INP-bglX fusion RBS 611..614 /label=rbs misc_feature 3911..3938 /label=Spacer misc_feature 3920..3920 /label=TAA promoter 286..291 /label=-35 promoter 309..314 /label=-10 RBS 348..351 /label=rbs misc_feature 1565..1600 /label=Spacer (Linker) ORIGIN 1 ttaatacgac tcactatagg gagaggaggt acgatctgct ggggcaaacc agcgtggacc 61 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 121 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 181 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 241 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 301 ccggctcgta tgttgtgtga aattgtgagc ggataacaat ttcacacagg aaacagctat 361 gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 421 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 481 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt 541 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgat actagctgat 601 atttcgcgta aggaaatcca ttatgggatc tatgaccctg gataaagcgc tggtgctgcg 661 cacttgtgcc aataatatgg ccgaccattg tggcctgatc tggcctgctt ctggtacagt 721 tgaaagccgc tattggcagt ctacacgtcg tcacgaaaac gggctggttg ggctgctgtg 781 gggtgccgga acatccgcct ttctgagcgt tcatgccgat gctcgttgga ttgtttgtga 841 ggttgctgtt gccgacatca ttagcctgga agaaccgggt atggtgaaat ttcctcgtgc 901 tgaagtggtc catgttggtg atcgtattag cgcctcccat ttcatttcag ctcgtcaggc 961 tgatccagca agcacatcaa caagcacctc taccagtaca ctgacaccaa tgccaaccgc 1021 tatccctact cctatgccgg ctgtagcctc tgttactctg cctgtggccg aacaagctcg 1081 ccacgaagtc tttgacgttg cctctgtgtc tgctgctgct gctcctgtta acactctgcc 1141 tgttaccacg cctcaaaatc tgcaaacggc gacctatggc tcaacactga gtggtgataa 1201 ccattctcgt ctgattgccg ggtatggctc taacgaaacc gccggaaacc attctgatct 1261 gatcggtggt cacgattgta cgctgatggc aggtgatcaa tctcgtctga ccgctggcaa 1321 aaattccgtt ctgaccgctg gggcacgttc taaactgatc gggagcgaag gctctactct 1381 gtccgccgga gaagattcta ccctgatctt tcgtctgtgg gatggtaaac gttatcgtca 1441 gctggtcgct cgtacaggtg aaaatggtgt ggaagccgac attccgtatt atgtgaatga 1501 ggacgatgat attgtggaca aaccggacga agatgatgat tggatcgaag tgaaaggtac 1561 tagcagcagc attgcgagca gcagcccgag cagcgtggcg ggatctatga aatggctatg 1621 ttcagtagga atcgcggtga gtctggccct gcaaccagca ctggcggatg atttattcgg 1681 caaccatcca ttaacgcccg aagcgcggga tgcgttcgtc accgaactgc ttaagaaaat 1741 gacagttgat gagaaaattg gtcagctgcg cttaatcagc gtcggcccgg ataacccgaa 1801 agaggcgatc cgcgagatga tcaaagacgg tcaggttggg gcgattttca acaccgtaac 1861 ccgtcaggat atccgcgcca tgcaggatca ggtgatggaa ttaagccgcc tgaaaattcc 1921 tcttttcttt gcttacgacg tgctgcacgg tcagcgcacg gtgttcccga ttagcctcgg 1981 tctggcctcg tcttttaacc tcgatgcagt gaaaacggtc ggacgtgtct ctgcttatga 2041 agcggcagat gatggcctga atatgacctg ggcaccgatg gtcgatgtct cgcgcgatcc 2101 gcgctgggga cgtgcttccg aaggttttgg cgaagatacg tatctcacct caacaatggg 2161 taaaaccatg gtggaagcga tgcagggtaa aagcccggca gatcgctact cggtgatgac 2221 cagcgtcaaa cactttgccg catacggcgc ggtagaaggc ggtaaagagt acaacaccgt 2281 cgatatgagt ccgcagcgcc tgtttaatga ttatatgccg ccgtacaaag cggggctgga 2341 cgcaggcagc ggcgcggtga tggtggcgct gaactcgctg aacggcacgc cagccacctc 2401 cgattcctgg ctgctgaaag atgttctgcg cgaccagtgg ggctttaaag gcatcaccgt 2461 ttccgatcac ggtgcaatca aagagctgat taaacatggc acggcggcag acccggaaga 2521 tgcggtgcgc gtggcgctga aatccggaat caacatgagc atgagcgacg agtactactc 2581 gaagtatctg cctgggttga ttaaatccgg caaagtgacg atggcagagc tggacgatgc 2641 tgcccgccat gtactgaacg ttaaatatga tatggggttg tttaacgacc catacagcca 2701 tttggggccg aaagagtctg acccggtgga taccaatgcc gaaagccgcc tgcaccgtaa 2761 agaagcgcgt gaagtggcgc gcgaaagctt ggtgttgctg aaaaaccgtc tcgaaacgtt 2821 accgctgaaa aaatcggcca ccattgcggt ggttgggcca ctggcggaca gtaaacgtga 2881 cgtgatgggc agctggtccg cagccggtgt tgccgatcaa tccgtgaccg tactgaccgg 2941 gattaaaaat gccgtcggtg aaaacggtaa agtgctgtat gccaaagggg cgaacgttac 3001 cagtgacaaa ggcattatcg atttcctgaa tcagtatgaa gaagcggtca aagtcgatcc 3061 gcgttcgccg caagagatga ttgatgaagc ggtgcagacg gcgaaacaat ctgatgtggt 3121 ggtggctgta gtcggtgaag cacaggggat ggcgcacgaa gcctccagcc ggaccgatat 3181 cactattccg caaagccaac gtgacttgat tgcggcgctg aaagccaccg gtaaaccgct 3241 ggtgctggtg ctgatgaacg ggcgtccgct ggcgctggtg aaagaagatc agcaggctga 3301 tgcgattctg gaaacctggt ttgcggggac tgaaggcggt aatgcaattg ccgatgtatt 3361 gtttggcgat tacaacccgt ccggcaagct gccaatgtcc ttcccgcgtt ctgtcgggca 3421 gatcccggtg tactacagcc atctgaatac cggtcgcccg tataatgccg acaagccgaa 3481 caaatacact tcgcgttatt ttgatgaagc taacggggcg ttgtatccgt tcggctatgg 3541 gctgagctac accactttca ccgtctctga tgtgaaactt tctgcgccga ccatgaagcg 3601 tgacggcaaa gtgactgcca gcgtgcaggt gacgaacacc ggtaagcgcg agggtgccac 3661 ggtagtgcag atgtacttgc aggatgtgac tgcttccatg agtcgccctg tgaaacagct 3721 gaaaggcttt gagaaaatca ccctgaaacc gggcgaaact cagactgtca gcttcccgat 3781 cgatattgag gcgctgaagt tctggaatca acagatgaaa tatgacgccg agcctggcaa 3841 gttcaatgtc tttatcggca ctgattccgc acgcgttaag aaaggcgagt ttgagttgct 3901 gggtactagc aacatatcat aacggagtga tcgcaatggg atctgttgtt tgtcggtgaa 3961 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //