LOCUS BBa_K523022 4145 bp DNA linear 22-Dec-2024 DEFINITION PlacLacZ + crtEIB ACCESSION BBa_K523022 VERSION BBa_K523022.1 FEATURES Location/Qualifiers misc_feature 38..589 /label=Plac - LacZ misc_feature 1..32 /label=Spacer CDS 359..589 /label=LacZ promoter 286..291 /label=-35 promoter 309..314 /label=-10 RBS 348..351 /label=rbs misc_feature 597..625 /label=Spacer RBS 611..614 /label=rbs misc_feature 623..623 /label=ATG CDS 623..1558 /label=crtE misc_feature 1556..1556 /label=TAA misc_feature 1547..1574 /label=Spacer misc_feature 1572..1572 /label=ATG CDS 1572..3068 /label=crtI misc_feature 3066..3066 /label=TGA misc_feature 3066..3095 /label=Spacer RBS 3088..3091 /label=rbs CDS 3102..4040 /label=crtB misc_feature 4038..4038 /label=TAG misc_feature 4038..4067 /label=Spacer misc_feature 3102..3102 /label=ATG RBS 1560..1563 /label=rbs ORIGIN 1 ttaatacgac tcactatagg gagaggaggt acgatctgct ggggcaaacc agcgtggacc 61 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 121 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 181 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 241 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 301 ccggctcgta tgttgtgtga aattgtgagc ggataacaat ttcacacagg aaacagctat 361 gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 421 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 481 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt 541 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgat actagctgat 601 atttcgcgta aggaaatcca ttatgggatc tatgacggtc tgcgcaaaaa aacacgttca 661 tctcactcgc gatgctgcgg agcagttact ggctgatatt gatcgacgcc ttgatcagtt 721 attgcccgtg gagggagaac gggatgttgt gggtgccgcg atgcgtgaag gtgcgctggc 781 accgggaaaa cgtattcgcc ccatgttgct gttgctgacc gcccgcgatc tgggttgcgc 841 tgtcagccat gacggattac tggatttggc ctgtgcggtg gaaatggtcc acgcggcttc 901 gctgatcctt gacgatatgc cctgcatgga cgatgcgaag ctgcggcgcg gacgccctac 961 cattcattct cattacggag agcatgtggc aatactggcg gcggttgcct tgctgagtaa 1021 agcctttggc gtaattgccg atgcagatgg cctcacgccg ctggcaaaaa atcgggcggt 1081 ttctgaactg tcaaacgcca tcggcatgca aggattggtt cagggtcagt tcaaggatct 1141 gtctgaaggg gataagccgc gcagcgctga agctattttg atgacgaatc actttaaaac 1201 cagcacgctg ttttgtgcct ccatgcagat ggcctcgatt gttgcgaatg cctccagcga 1261 agcgcgtgat tgcctgcatc gtttttcact tgatcttggt caggcatttc aactgctgga 1321 cgatttgacc gatggcatga ccgacaccgg taaggatagc aatcaggacg ccggtaaatc 1381 gacgctggtc aatctgttag gcccgagggc ggttgaagaa cgtctgagac aacatcttca 1441 gcttgccagt gagcatctct ctgcggcctg ccaacacggg cacgccactc aacattttat 1501 tcaggcctgg tttgacaaaa aactcgctgc cgtcagtggt actagcaaca tatcataacg 1561 gagtgatcgc aatgggatct atgaaaccaa ctacggtaat tggtgcaggc ttcggtggcc 1621 tggcactggc aattcgtcta caagctgcgg ggatccccgt cttactgctt gaacaacgtg 1681 ataaacccgg cggtcgggct tatgtctacg aggatcaggg gtttaccttt gatgcaggcc 1741 cgacggttat caccgatccc agtgccattg aagaactgtt tgcactggca ggaaaacagt 1801 taaaagagta tgtcgaactg ctgccggtta cgccgtttta ccgcctgtgt tgggagtcag 1861 ggaaggtctt taattacgat aacgatcaaa cccggctcga agcgcagatt cagcagttta 1921 atccccgcga tgtcgaaggt tatcgtcagt ttctggacta ttcacgcgcg gtgtttaaag 1981 aaggctatct aaagctcggt actgtccctt ttttatcgtt cagagacatg cttcgcgccg 2041 cacctcaact ggcgaaactg caagcatgga gaagcgttta cagtaaggtt gccagttaca 2101 tcgaagatga acatctgcgc caggcgtttt ctttccactc gctgttggtg ggcggcaatc 2161 ccttcgccac ctcatccatt tatacgttga tacacgcgct ggagcgtgag tggggcgtct 2221 ggtttccgcg tggcggcacc ggcgcattag ttcaggggat gataaagctg tttcaggatc 2281 tgggtggcga agtcgtgtta aacgccagag tcagccatat ggaaacgaca ggaaacaaga 2341 ttgaagccgt gcatttagag gacggtcgca ggttcctgac gcaagccgtc gcgtcaaatg 2401 cagatgtggt tcatacctat cgcgacctgt taagccagca ccctgccgcg gttaagcagt 2461 ccaacaaact gcaaactaag cgcatgagta actctctgtt tgtgctctat tttggtttga 2521 atcaccatca tgatcagctc gcgcatcaca cggtttgttt cggcccgcgt taccgcgagc 2581 tgattgacga aatttttaat catgatggcc tcgcagagga cttctcactt tatctgcacg 2641 cgccctgtgt cacggattcg tcactggcgc ctgaaggttg cggcagttac tatgtgttgg 2701 cgccggtgcc gcatttaggc accgcgaacc tcgactggac ggttgagggg ccaaaactac 2761 gcgaccgtat ttttgcgtac cttgagcagc attacatgcc tggcttacgg agtcagctgg 2821 tcacgcaccg gatgtttacg ccgtttgatt ttcgcgacca gcttaatgcc tatcatggct 2881 cagccttttc tgtggagccc gttcttaccc agagcgcctg gtttcggccg cataaccgcg 2941 ataaaaccat tactaatctc tacctggtcg gcgcaggcac gcatcccggc gcaggcattc 3001 ctggcgtcat cggctcggca aaagcgacag caggtttgat gctggaggat ctgataggta 3061 ctagctgaac gtaaatactc agtaaagagg agaaaggatc tatgaataat ccgtcgttac 3121 tcaatcatgc ggtcgaaacg atggcagttg gctcgaaaag ttttgcgaca gcctcaaagt 3181 tatttgatgc aaaaacccgg cgcagcgtac tgatgctcta cgcctggtgc cgccattgtg 3241 acgatgttat tgacgatcag acgctgggct ttcaggcccg gcagcctgcc ttacaaacgc 3301 ccgaacaacg tctgatgcaa cttgagatga aaacgcgcca ggcctatgca ggatcgcaga 3361 tgcacgaacc ggcgtttgcg gcttttcagg aagtggctat ggctcatgat atcgccccgg 3421 cttacgcgtt tgatcatctg gaaggcttcg ccatggatgt acgcgaagcg caatacagcc 3481 aactggatga tacgctgcgc tattgctatc acgttgcagg cgttgtcggc ttgatgatgg 3541 cgcaaatcat gggcgtgcgg gataacgcca cgctggaccg cgcctgtgac cttgggctgg 3601 catttcagtt gaccaatatt gctcgcgata ttgtggacga tgcgcatgcg ggccgctgtt 3661 atctgccggc aagctggctg gagcatgaag gtctgaacaa agagaattat gcggcacctg 3721 aaaaccgtca ggcgctgagc cgtatcgccc gtcgtttggt gcaggaagca gaaccttact 3781 atttgtctgc cacagccggc ctggcagggt tgcccctgcg ttccgcctgg gcaatcgcta 3841 cggcgaagca ggtttaccgg aaaataggtg tcaaagttga acaggccggt cagcaagcct 3901 gggatcagcg gcagtcaacg accacgcccg aaaaattaac gctgctgctg gccgcctctg 3961 gtcaggccct tacttcccgg atgcgggctc atcctccccg ccctgcgcat ctctggcagc 4021 gcccgctcgg tactagctag gtcttagtct tcgtcaaaga ggggacagga tctgttgttt 4081 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 4141 ttata //