##gff-version 3
##sequence-region BBa_K523022 1 4067
BBa_K523022	.	promoter	309	314	.	+	0	ID=annotation2129064;Name=-10
BBa_K523022	.	promoter	286	291	.	+	0	ID=annotation2129063;Name=-35
BBa_K523022	.	CDS	3102	4040	.	+	0	ID=annotation2129078;Name=crtB
BBa_K523022	.	CDS	359	589	.	+	0	ID=annotation2129062;Name=LacZ
BBa_K523022	.	sequence_feature	597	625	.	+	0	ID=annotation2129067;Name=Spacer
BBa_K523022	.	start_codon	3102	3102	.	+	0	ID=annotation2129081;Name=ATG
BBa_K523022	.	ribosome_entry_site	1560	1563	.	+	0	ID=annotation2129082;Name=rbs
BBa_K523022	.	ribosome_entry_site	611	614	.	+	0	ID=annotation2129068;Name=rbs
BBa_K523022	.	start_codon	1572	1572	.	+	0	ID=annotation2129073;Name=ATG
BBa_K523022	.	ribosome_entry_site	348	351	.	+	0	ID=annotation2129065;Name=rbs
BBa_K523022	.	sequence_feature	4038	4067	.	+	0	ID=annotation2129080;Name=Spacer
BBa_K523022	.	CDS	623	1558	.	+	0	ID=annotation2129070;Name=crtE
BBa_K523022	.	stop_codon	4038	4038	.	+	0	ID=annotation2129079;Name=TAG
BBa_K523022	.	sequence_feature	3066	3095	.	+	0	ID=annotation2129076;Name=Spacer
BBa_K523022	.	stop_codon	1556	1556	.	+	0	ID=annotation2129071;Name=TAA
BBa_K523022	.	stop_codon	3066	3066	.	+	0	ID=annotation2129075;Name=TGA
BBa_K523022	.	sequence_feature	1	32	.	+	0	ID=annotation2129061;Name=Spacer
BBa_K523022	.	ribosome_entry_site	3088	3091	.	+	0	ID=annotation2129077;Name=rbs
BBa_K523022	.	CDS	1572	3068	.	+	0	ID=annotation2129074;Name=crtI
BBa_K523022	.	sequence_feature	38	589	.	+	0	ID=annotation2129059;Name=Plac - LacZ
BBa_K523022	.	sequence_feature	1547	1574	.	+	0	ID=annotation2129072;Name=Spacer
BBa_K523022	.	start_codon	623	623	.	+	0	ID=annotation2129069;Name=ATG
>BBa_K523022
ttaatacgactcactatagggagaggaggtacgatctgctggggcaaaccagcgtggaccgcttgctgcaactctctca
gggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaa
accgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgag
cgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgt
gtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttac
aacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaa
tagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccg
gcaccagaagcggtgccggaaagctggctggagtgatactagctgatatttcgcgtaaggaaatccattatgggatcta
tgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgcct
tgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaa
cgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttgg
cctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcg
cggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagccttt
ggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggca
tgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgac
gaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcg
cgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccg
acaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacg
tctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttatt
caggcctggtttgacaaaaaactcgctgccgtcagtggtactagcaacatatcataacggagtgatcgcaatgggatct
atgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccg
tcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcagg
cccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaa
ctgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggc
tcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtt
taaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcg
aaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctt
tccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtg
gggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaa
gtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgca
ggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgc
ggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccat
catgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatg
gcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcag
ttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgac
cgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgt
ttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtt
tcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcct
ggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgataggtactagctgaacgtaaatactca
gtaaagaggagaaaggatctatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaag
ttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgt
gacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgc
aacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggc
tatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatac
agccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcg
tgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgt
ggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcg
gcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgcca
cagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgt
caaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctg
gccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctcg
gtactagctaggtcttagtcttcgtcaaagaggggacaggatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttata