LOCUS       BBa_K523023             1458 bp    DNA     linear       16-May-2024
DEFINITION  PlacLacZ + EYFP
ACCESSION   BBa_K523023
VERSION     BBa_K523023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..32
                     /label=Spacer
     misc_feature    38..589
                     /label=Plac - LacZ
     promoter        286..291
                     /label=-35
     promoter        309..314
                     /label=-10
     CDS             359..589
                     /label=LacZ
     misc_feature    447..447
                     /label=GCA to GTA
     misc_feature    597..625
                     /label=Spacer
     RBS             611..614
                     /label=rbs
     CDS             623..1345
                     /label=EYFP
     misc_feature    1340..1340
                     /label=TAATAA
     RBS             348..351
                     /label=rbs
ORIGIN
        1 ttaatacgac tcactatagg gagaggaggt acgatctgct ggggcaaacc agcgtggacc
       61 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
      121 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
      181 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      241 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      301 ccggctcgta tgttgtgtga aattgtgagc ggataacaat ttcacacagg aaacagctat
      361 gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      421 cgttacccaa cttaatcgcc ttgcagtaca tccccctttc gccagctggc gtaatagcga
      481 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
      541 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgat actagctgat
      601 atttcgcgta aggaaatcca ttatggtgag caagggcgag gagctgttca ccggggtggt
      661 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      721 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      781 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc
      841 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta
      901 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      961 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1021 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1081 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1141 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1201 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     1261 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1321 catggacgag ctgtacaagt aataatacta gcaacatatc ataacggagt gatcgcaatg
     1381 ggatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1441 ggcctttctg cgtttata
//