LOCUS       BBa_K524000             2067 bp    DNA     linear       07-May-2024
DEFINITION  Heat sensitive origin of replication (oriR101 & repA101-ts)
ACCESSION   BBa_K524000
VERSION     BBa_K524000.1  
FEATURES             Location/Qualifiers
     CDS             2..184
                     /label=orf60a CDS
     CDS             373..1323
                     /label=repA101-ts
     misc_feature    1492..1574
                     /label=AT Rich region_oriR101
     promoter        1329..1354
                     /label=Promoter region of repA101ts
     misc_feature    1391..1391
                     /label=point mutation, substitute of C as G to remove 
                     SpeI site
     misc_feature    1721..2067
                     /label=par region
     misc_feature    1..372
                     /label=regions not necessary for origin function
ORIGIN
        1 gatgacgcat cctcacgata atatccgggt aggcgcaatc actttcgtct actccgttac
       61 aaagcgaggc tgggtatttc ccggcctttc tgttatccga aatccactga aagcacagcg
      121 gctggctgag gagataaata ataaacgagg ggctgtatgc acaaagcatc ttctgttgag
      181 ttaagaacga gtatcgagat ggcacatagc cttgctcaaa ttggaatcag gtttgtgcca
      241 ataccagtag aaacagacga agaatccatg ggtatggaca gttttccctt tgatatgtaa
      301 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga
      361 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg
      421 tttttgcgtg agccatgaga acgaaccatt gagatcatac ttactttgca tgtcactcaa
      481 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt
      541 tcttagtccg ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt
      601 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac
      661 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct
      721 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc
      781 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt
      841 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga
      901 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg
      961 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt
     1021 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac
     1081 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact
     1141 gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt
     1201 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg
     1261 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga
     1321 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca
     1381 atgataatta gtagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt
     1441 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt
     1501 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat
     1561 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta
     1621 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg
     1681 cttaagtagc accctcgcaa gctcggttgc ggccgcaatc gggcaaatcg ctgaatattc
     1741 cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct
     1801 gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt
     1861 catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt
     1921 atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct
     1981 gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc
     2041 acccagtaag gcagcggtat catcaac
//