##gff-version 3
##sequence-region BBa_K524000 1 2067
BBa_K524000	.	sequence_feature	1721	2067	.	+	0	ID=annotation2371776;Name=par region
BBa_K524000	.	sequence_feature	1	372	.	+	0	ID=annotation2371777;Name=regions not necessary for origin function
BBa_K524000	.	promoter	1329	1354	.	+	0	ID=annotation2138825;Name=Promoter region of repA101ts
BBa_K524000	.	sequence_feature	1492	1574	.	+	0	ID=annotation2138824;Name=AT Rich region_oriR101
BBa_K524000	.	CDS	373	1323	.	+	0	ID=annotation2138823;Name=repA101-ts
BBa_K524000	.	CDS	2	184	.	+	0	ID=annotation2138822;Name=orf60a CDS
BBa_K524000	.	sequence_alteration	1391	1391	.	+	0	ID=annotation2138826;Name=point mutation, substitute of C as G to remove SpeI site
>BBa_K524000
gatgacgcatcctcacgataatatccgggtaggcgcaatcactttcgtctactccgttacaaagcgaggctgggtattt
cccggcctttctgttatccgaaatccactgaaagcacagcggctggctgaggagataaataataaacgaggggctgtat
gcacaaagcatcttctgttgagttaagaacgagtatcgagatggcacatagccttgctcaaattggaatcaggtttgtg
ccaataccagtagaaacagacgaagaatccatgggtatggacagttttccctttgatatgtaacggtgaacagttgttc
tacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagcca
gtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtc
actcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgtta
cgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggtta
cgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaattt
catattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagtta
ttttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagtt
cttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaa
tttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatag
tttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttg
ctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgt
ccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctcc
gttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggt
gattttaatcactataccaattgagatgggctagtcaatgataattagtagtccttttcctttgagttgtgggtatctg
taaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgtt
ttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagcc
ctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaa
cagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcggttgcggccgcaatcgggcaaatcgctgaatat
tccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggc
agtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagccc
gtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgc
cctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcag
cggtatcatcaac