BBa_K524004 1 BBa_K524004 pir gene 2011-10-02T11:00:00Z 2015-05-08T01:12:34Z from the gDNA of E.coli strain BL21 ori-gamma is part of the replication origin of the R6K origin. Initiation of replication at ori-gamma requires the pi protein in trans, which is encoded by the pir gene. Yet doubling the concentration of pi protein would effectively shut down the replication as well. Expression of pi protein is auto-regulated at its promoter region. Our construct design was to insert a CAP-binding site between the auto-regulating promoter and the CDS, by doing so we can halt replication and induce plasmid loss in nutrient (glucose) rich medium. false false _689_ 0 8756 9 In stock true We modified the oriniginal pir gene from BL21 by inserting a CAP-binding site between the auto-regulating promoter and the CDS false HO Yuan Heng Trevor annotation2150869 1 regulatory binding site for pi protein range2150869 1 1 57 annotation2150871 1 pir gene range2150871 1 91 1008 annotation2150872 1 start codon range2150872 1 91 93 annotation2150870 1 autogenously regulated promoter range2150870 1 24 90 annotation2150873 1 stop codon range2150873 1 1006 1008 BBa_K524004_sequence 1 catgagtggatagtacgttgctaaaacatgagataaaaattgactctcatgttattggcgttaagatatacagaatgatgaggtttttttatgagactcaaggtcatgatggacgtgaacaaaaaaacgaaaattcgccaccgaaacgagctaaatcacaccctggctcaacttcctttgcccgcaaagcgagtgatgtatatggcgcttgctcccattgatagcaaagaacctcttgaacgagggcgagttttcaaaattagggctgaagaccttgcagcgctcgccaaaatcaccccatcgcttgcttatcgacaattaaaagagggtggtaaattacttggtgccagcaaaatttcgctaagaggggatgatatcattgctttagctaaagagcttaacctgccctttactgctaaaaactcccctgaagagttagatcttaacattattgagtggatagcttattcaaatgatgaaggatacttgtctttaaaattcaccagaaccatagaaccatatatctctagccttattgggaaaaaaaataaattcacaacgcaattgttaacggcaagcttacgcttaagtagccagtattcatcttctctttatcaacttatcaggaagcattactctaattttaagaagaaaaattattttattatttccgttgatgagttaaaggaagagttaatagcttatacttttgataaagatggaaatattgagtacaaataccctgactttcctatttttaaaagggatgtgttaaataaagccattgctgaaattaaaaagaaaacagaaatatcgtttgttggcttcactgttcatgaaaaagaaggaagaaaaattagtaagctgaagttcgaatttgtcgttgatgaagatgaattttctggcgataaagatgatgaagctttttttatgaatttatctgaagctgatgcagcttttctcaaggtatttgatgaaaccgtacctcccaaaaaagctaaggggtgatatatggctaaaatttacgatttccctcaaggagccgaacgccgcaggatgcaccgcaaaatccagtggaacaacgctgtaaaattatctaaaaatggctggagtaagccagaggttaaacgctggtcttttttagcattcatctcaactggct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z