BBa_K524203 1 BBa_K524203 pSC101 heat sensitive origin of replication with deletion in par locus 2012-08-25T11:00:00Z 2015-05-08T01:12:34Z Modified from BBa_K524000 A derivative of pSC101 origin of replication, this heat sensitive origin has the par locus deleted, therefore allowing a rapid loss of plasmid even under the permissive temperature. false false _689_ 0 8758 9 Not in stock false The par locus was deleted through excision of BBa_K524000 by SspI and StuI, purification of desired fragments and then false HO Yuan Heng Trevor annotation2181033 1 DnaD protein binding sequence range2181033 1 1582 1590 annotation2181031 1 IHF binding sequence range2181031 1 1514 1526 annotation2181021 1 repA101ts range2181021 1 373 1323 annotation2181022 1 Inverted Repeat 2 (IR2) -> range2181022 1 1325 1348 annotation2181030 1 Direct Repeat 1 (DR1) range2181030 1 1474 1494 annotation2181024 1 -10 promoter region of repAts range2181024 1 1354 1359 annotation2181034 1 scar of removed par locus range2181034 1 1734 1739 annotation2181026 1 Inverted Repeat 1 (IR1) -> range2181026 1 1371 1384 annotation2181028 1 Direct Repeat 3 (DR3) range2181028 1 1420 1441 annotation2181029 1 Direct Repeat 2 (DR2) range2181029 1 1453 1473 annotation2181023 1 Inverted Repeat 2 (IR2) <- range2181023 1 1349 1371 annotation2181027 1 Inverted Repeat 1 (IR1) <- range2181027 1 1385 1397 annotation2181025 1 -35 promoter region of repAts range2181025 1 1376 1381 annotation2181032 1 AT Rich Region range2181032 1 1492 1547 BBa_K524203_sequence 1 gatgacgcatcctcacgataatatccgggtaggcgcaatcactttcgtctactccgttacaaagcgaggctgggtatttcccggcctttctgttatccgaaatccactgaaagcacagcggctggctgaggagataaataataaacgaggggctgtatgcacaaagcatcttctgttgagttaagaacgagtatcgagatggcacatagccttgctcaaattggaatcaggtttgtgccaataccagtagaaacagacgaagaatccatgggtatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattagtagtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcggttgcggccgcaatcgggcaaatcgctgaatcctgcattatgtcgtgattgagcgggatgaaaagtacatggcgagttttgacgagatcgtgccggagttcatcgaaaaaatggacgaggcactggctgaaattggttttgtatttggggagcaatggcgctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z