LOCUS BBa_K525001 4746 bp DNA linear 22-Nov-2024 DEFINITION Fusion protein of SgsE, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac ACCESSION BBa_K525001 VERSION BBa_K525001.1 FEATURES Location/Qualifiers promoter 1..20 /label=T7 promoter misc_feature 20..44 /label=lac operator misc_feature 53..64 /label=B0034 RBS 56..59 /label=RBS misc_feature 73..75 /label=ATG misc_feature 73..2400 /label=K525301 misc_feature 2401..2406 /label=Freiburg Scar misc_feature 2407..4746 /label=K525560 misc_feature 3148..3153 /label=Freiburg Scar misc_feature 3466..3471 /label=Freiburg Scar misc_feature 4744..4746 /label=TAA misc_feature 4738..4743 /label=AgeI CDS 3472..4746 /label=BisdB CDS 3154..3465 /label=BisdA CDS 2407..3147 /label=Reductase CDS 76..2400 /label=SgsE domain ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcctactag agaaagagga 61 gaaatactag agatggccgg cgctaccaag ctcgacaaga tgcgccagga gctgaaggca 121 gctgttgacg caaaggatct caagaaggct gaggaactgt accacaagat ctcctacgag 181 cttaagaccc gtactgttat cctcgaccgc gtctacggcc agtccacccg tgagctgctc 241 cgctccacct tcaaggcaga cgctcaggca ctgcgtgatt ctcttatcta cgacatcacc 301 gttgctatga aggcacgcga agctcaggac gcagttaagg ctggtaacct cgataaggca 361 aaggctgcac tggaccaggt caaccagtac gtttccaagg ttactgacgc tttcaaggca 421 gagctccaga aggctgcaca ggatgctaag gcagcttacg aggcagctct gccaccaaag 481 gtcgaatccg tgaccgcagt taacgctaag acccttgaga tcaagttcaa caaggcagtt 541 gacgctgcaa ccgtcatcga caacaagggc acttccgata cctctgacga cgttgttaag 601 gctaccgcaa tcaccctcaa ggctatcgat gaccaggtcc ctgtttccac tgttaaggca 661 tccctgtccg acgataagaa gaccctcaag ctggtcgtgg acggcgctca gttcttcacc 721 aagcgttacg ttgttgacat caagaacgtc aagacccttg atggtaagga cgttccagca 781 tacactacca ccatcgacac cactgattct gttcgcccat ccgtcctctc cttctcctac 841 gctgacaacg gcctgaccct caaggttaag ttctctgagc ctctggcatc cgttggcacc 901 gtcaagcttt acgacggtac cactgaaatc tccgtgtccc caaagttcac cgctggcgat 961 gacgagatga ccatcaacct cgcatcttcc tccgttccag ttaacaagga cctgaccctc 1021 aagatcttcg gcgctgtcga ttacaacggt aacgttatca accctaaccc agcagagctg 1081 actgttaaga agaccaccgt cgacatcacc aagccagttg ttcagtccat cgaagctgtc 1141 aacactaaga ccgtgaaggt taccttctct gagaagcttc tctccgcacc taccatcaag 1201 atcggcggcc agactgcttc cgtttccgtc gactctactg gtctggttta caccgcaacc 1261 ctcgcttccg cactgtccaa gggcgtttac gctgtcgagg tttccgatta caaggacctt 1321 gcaggcaact ctggtgacgc ttacactaag gttgtccagc tcaaggcaga taacaccgct 1381 ccaaagttcg tgtcctccca ggttgttaag atcaacggcg tcgaacacct ggttctcacc 1441 ttcgacgagg aggttaccac tggctccaac atcaccgtcg ttcagtcttc cgacaagtac 1501 atcgatgaaa acaacgttct gaaggcagtc ggtgctgacc ttaagaccac ctccgacaac 1561 ttcaagctct acctgccaac tgatggcaag tccaagtctg tggcactcaa catctcctcc 1621 ctgcctaagg gcacctacac cgttaccctt ccaaacggtc tcgtttccga cctggctgac 1681 aacccatacg cagagcgtaa gcagatcact ttcgtccgcg gctctgattc cctcaccacc 1741 aagcctgctc tggacaagga ctacgattcc aacggcgtta aggcagacaa caacaacgag 1801 cttgttttcg ctttcaccca gaacctcgac gcatccgctc tgaacctctc taacttcaac 1861 atcaacggtc tgactgtcac caaggcagtt ttcgatggcg acaccaagca catccgtgtt 1921 acccttgctc caggcgcaaa cacttggact ggtacccacg tcatcaccat ctccaacatc 1981 aagaacactt ccggcctcgt gatggacacc gttaccgtta acgaatacat gaaggagaac 2041 gtcgctccaa ccttcactgc aaccctgacc tccgctgatg ttatccgcgt tgacttctct 2101 gagcctgtcg caaacgctac catctcccgt gcactctccg ctaacaactt catcgttaag 2161 gttgacggca acgtcgtgac tgtttccaac gtttacgaag ataacaacgc aaccaacctg 2221 gtccagggtt ctaagggcta caagaccgtt taccttaagc tccagtcccc agttaccgac 2281 ctgtccaagc caatcactct ctccgctacc gacatcgtcg atgttaacca gaccggcgca 2341 atcaccgaca acaacgttgt cggtaacaac gtgtctgcta ctgttgttaa cgtcgcaaag 2401 accggcgctg attgggtaac aggcaaagtc actaaagtgc agaactggac cgacgccctg 2461 tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg ccgggcaatt taccaagctt 2521 ggccttgaaa tcgacggcga acgcgtccag cgcgcctact cctatgtaaa ctcgcccgat 2581 aatcccgatc tggagtttta cctggtcacc gtccccgatg gcaaattaag cccacgactg 2641 gcggcactga aaccaggcga tgaagtgcag gtggttagcg aagcggcagg attctttgtg 2701 ctcgatgaag tgccgcactg cgaaacgcta tggatgctgg caacgggcac agcgattggc 2761 ccttatttat cgattctgca actaggtaaa gatttagatc gcttcaaaaa tctggtcctg 2821 gtgcacgccg cacgttatgc cgccgactta agctatttgc cactgatgca ggaactggaa 2881 aaacgctacg aaggaaaact gcgcattcag acggtggtca gtcgggaaac ggcagcgggg 2941 tcgctcaccg gacggatacc ggcattaatt gaaagtgggg aactggaaag cacgattggc 3001 ctgccgatga ataaagaaac cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc 3061 gatacacaac agttgctgaa agagacccgg cagatgacga aacatttacg tcgccgaccg 3121 ggccatatga cagcggagca ttactggacc ggccctcata tccaagttac gactcgtgat 3181 ggtgaaattc gcgagctgga cgttgcagct tccggtttcc tgatggaagc gctgcgtgac 3241 gctaacatcg atggtgtgga ggcgatgtgt ggcggttgct gttcttgcgc gacttgccac 3301 gtctacattg acgcggctcc agccggtacc ctgccgccgg tgtctagcga tgaagaaatg 3361 ctgctgtccg gcctggtttc taccccgggc cgctctcgtc tgtcttgcca gatcccggta 3421 accgcagaac tggacggcct gaaactgacc atcccgccgg atagcaccgg caacccacaa 3481 actctgccgg tatttccaga cctggatatc ttcagcccgg aatacgcttg taaccgcgaa 3541 aaatatgcag cgcgtgcgct gcgcgattac ccgctgcact tttataaacc gctgaacctg 3601 tggattgtct ccaaacataa agacgttcgt agcgcgctgt tcaccccgca ggtcttcagc 3661 tccgtagctt ttggtctgct gcctccacca gacgatatcg ctcctcgcgt gccggacctg 3721 tacaccgacg ttcatctgcc gtccatggat ccgccggagc acaccaaact gcgtgttccg 3781 gtgcagcagg ctctgctgcc gggccgtctg gtaggcaaag acgaggttgt gcgccgtatt 3841 gcgaacgaac tgatcgatac ttttatcgac aaaggcgaat gcgatctgct gcatgatttc 3901 tcttataagc tggcgctgta cctgattgta gacctgctgg gtatccctaa agaacgtgcg 3961 gaggactacc accgctggtc caactgtttc ttccagctgt tcacgccgaa ggttcctgaa 4021 cgtgcggatg ctcgtttctt tgtaccgatg ccggaggaag tgctgcgcca gaactgggaa 4081 ggtctggccg aagcaaatga ctatctgcgt gaagttgttg aaaacctgga ccgtaatccg 4141 ggcaacaaca tgctgtctaa cctgctgcaa ctgcgtgaac cggatggctc tcgtactatc 4201 actatctccg cgaacgtttg caacgccctg gaatttggcg cggcgggtca cgatacgacc 4261 gcaaccctga tcgctcacct gacttatttc gtgctgacca ccccggacct gaaggacact 4321 ctgaccgaag atccatctct gattccggca gccatctctg aaaccctgcg tcgtcgttgc 4381 ccggttgacg gtctgttccg ccgcaccctg agcgacgtag aactgtgcgg tcagaagatc 4441 gaatccggtt ctatcgtgta cctggacctg accgccgcta acctggatcc ggacgttttc 4501 ccggagccgg agactttccg tctgaaccgc gataacatta aagaaatggt gtctttcggc 4561 ttcggtcgtc acgtctgtgc aggtcagtac ctgagccgca tcgaagctaa agcagcatac 4621 gaagaactga tgcgtcgtat tcctaatatg cgtctggcag atggcttcaa actggagtac 4681 atgccgagcg tggccaccac tgttctgaaa ggcctgccgc tggtttggga taaaaacacc 4741 ggttaa //