##gff-version 3
##sequence-region BBa_K525001 1 4746
BBa_K525001	.	ribosome_entry_site	56	59	.	+	0	ID=annotation2160960;Name=RBS
BBa_K525001	.	start_codon	73	75	.	+	0	ID=annotation2160961;Name=ATG
BBa_K525001	.	sequence_feature	3148	3153	.	+	0	ID=annotation2160965;Name=Freiburg Scar
BBa_K525001	.	CDS	2407	3147	.	+	0	ID=annotation2161003;Name=Reductase
BBa_K525001	.	engineered_region	73	2400	.	+	0	ID=annotation2160962;Name=K525301
BBa_K525001	.	CDS	76	2400	.	+	0	ID=annotation2161004;Name=SgsE domain
BBa_K525001	.	sequence_feature	3466	3471	.	+	0	ID=annotation2160989;Name=Freiburg Scar
BBa_K525001	.	CDS	3472	4746	.	+	0	ID=annotation2160992;Name=BisdB
BBa_K525001	.	CDS	3154	3465	.	+	0	ID=annotation2161002;Name=BisdA
BBa_K525001	.	sequence_feature	2401	2406	.	+	0	ID=annotation2160963;Name=Freiburg Scar
BBa_K525001	.	promoter	1	20	.	+	0	ID=annotation2160957;Name=T7 promoter
BBa_K525001	.	operator	20	44	.	+	0	ID=annotation2160958;Name=lac operator
BBa_K525001	.	stop_codon	4744	4746	.	+	0	ID=annotation2160990;Name=TAA
BBa_K525001	.	non_covalent_binding_site	4738	4743	.	+	0	ID=annotation2160991;Name=AgeI
BBa_K525001	.	engineered_region	53	64	.	+	0	ID=annotation2160959;Name=B0034
BBa_K525001	.	engineered_region	2407	4746	.	+	0	ID=annotation2160964;Name=K525560
>BBa_K525001
taatacgactcactataggggaattgtgagcggataacaattcctactagagaaagaggagaaatactagagatggccg
gcgctaccaagctcgacaagatgcgccaggagctgaaggcagctgttgacgcaaaggatctcaagaaggctgaggaact
gtaccacaagatctcctacgagcttaagacccgtactgttatcctcgaccgcgtctacggccagtccacccgtgagctg
ctccgctccaccttcaaggcagacgctcaggcactgcgtgattctcttatctacgacatcaccgttgctatgaaggcac
gcgaagctcaggacgcagttaaggctggtaacctcgataaggcaaaggctgcactggaccaggtcaaccagtacgtttc
caaggttactgacgctttcaaggcagagctccagaaggctgcacaggatgctaaggcagcttacgaggcagctctgcca
ccaaaggtcgaatccgtgaccgcagttaacgctaagacccttgagatcaagttcaacaaggcagttgacgctgcaaccg
tcatcgacaacaagggcacttccgatacctctgacgacgttgttaaggctaccgcaatcaccctcaaggctatcgatga
ccaggtccctgtttccactgttaaggcatccctgtccgacgataagaagaccctcaagctggtcgtggacggcgctcag
ttcttcaccaagcgttacgttgttgacatcaagaacgtcaagacccttgatggtaaggacgttccagcatacactacca
ccatcgacaccactgattctgttcgcccatccgtcctctccttctcctacgctgacaacggcctgaccctcaaggttaa
gttctctgagcctctggcatccgttggcaccgtcaagctttacgacggtaccactgaaatctccgtgtccccaaagttc
accgctggcgatgacgagatgaccatcaacctcgcatcttcctccgttccagttaacaaggacctgaccctcaagatct
tcggcgctgtcgattacaacggtaacgttatcaaccctaacccagcagagctgactgttaagaagaccaccgtcgacat
caccaagccagttgttcagtccatcgaagctgtcaacactaagaccgtgaaggttaccttctctgagaagcttctctcc
gcacctaccatcaagatcggcggccagactgcttccgtttccgtcgactctactggtctggtttacaccgcaaccctcg
cttccgcactgtccaagggcgtttacgctgtcgaggtttccgattacaaggaccttgcaggcaactctggtgacgctta
cactaaggttgtccagctcaaggcagataacaccgctccaaagttcgtgtcctcccaggttgttaagatcaacggcgtc
gaacacctggttctcaccttcgacgaggaggttaccactggctccaacatcaccgtcgttcagtcttccgacaagtaca
tcgatgaaaacaacgttctgaaggcagtcggtgctgaccttaagaccacctccgacaacttcaagctctacctgccaac
tgatggcaagtccaagtctgtggcactcaacatctcctccctgcctaagggcacctacaccgttacccttccaaacggt
ctcgtttccgacctggctgacaacccatacgcagagcgtaagcagatcactttcgtccgcggctctgattccctcacca
ccaagcctgctctggacaaggactacgattccaacggcgttaaggcagacaacaacaacgagcttgttttcgctttcac
ccagaacctcgacgcatccgctctgaacctctctaacttcaacatcaacggtctgactgtcaccaaggcagttttcgat
ggcgacaccaagcacatccgtgttacccttgctccaggcgcaaacacttggactggtacccacgtcatcaccatctcca
acatcaagaacacttccggcctcgtgatggacaccgttaccgttaacgaatacatgaaggagaacgtcgctccaacctt
cactgcaaccctgacctccgctgatgttatccgcgttgacttctctgagcctgtcgcaaacgctaccatctcccgtgca
ctctccgctaacaacttcatcgttaaggttgacggcaacgtcgtgactgtttccaacgtttacgaagataacaacgcaa
ccaacctggtccagggttctaagggctacaagaccgtttaccttaagctccagtccccagttaccgacctgtccaagcc
aatcactctctccgctaccgacatcgtcgatgttaaccagaccggcgcaatcaccgacaacaacgttgtcggtaacaac
gtgtctgctactgttgttaacgtcgcaaagaccggcgctgattgggtaacaggcaaagtcactaaagtgcagaactgga
ccgacgccctgtttagtctcaccgttcacgcccccgtgcttccgtttaccgccgggcaatttaccaagcttggccttga
aatcgacggcgaacgcgtccagcgcgcctactcctatgtaaactcgcccgataatcccgatctggagttttacctggtc
accgtccccgatggcaaattaagcccacgactggcggcactgaaaccaggcgatgaagtgcaggtggttagcgaagcgg
caggattctttgtgctcgatgaagtgccgcactgcgaaacgctatggatgctggcaacgggcacagcgattggccctta
tttatcgattctgcaactaggtaaagatttagatcgcttcaaaaatctggtcctggtgcacgccgcacgttatgccgcc
gacttaagctatttgccactgatgcaggaactggaaaaacgctacgaaggaaaactgcgcattcagacggtggtcagtc
gggaaacggcagcggggtcgctcaccggacggataccggcattaattgaaagtggggaactggaaagcacgattggcct
gccgatgaataaagaaaccagccatgtgatgctgtgcggcaatccacagatggtgcgcgatacacaacagttgctgaaa
gagacccggcagatgacgaaacatttacgtcgccgaccgggccatatgacagcggagcattactggaccggccctcata
tccaagttacgactcgtgatggtgaaattcgcgagctggacgttgcagcttccggtttcctgatggaagcgctgcgtga
cgctaacatcgatggtgtggaggcgatgtgtggcggttgctgttcttgcgcgacttgccacgtctacattgacgcggct
ccagccggtaccctgccgccggtgtctagcgatgaagaaatgctgctgtccggcctggtttctaccccgggccgctctc
gtctgtcttgccagatcccggtaaccgcagaactggacggcctgaaactgaccatcccgccggatagcaccggcaaccc
acaaactctgccggtatttccagacctggatatcttcagcccggaatacgcttgtaaccgcgaaaaatatgcagcgcgt
gcgctgcgcgattacccgctgcacttttataaaccgctgaacctgtggattgtctccaaacataaagacgttcgtagcg
cgctgttcaccccgcaggtcttcagctccgtagcttttggtctgctgcctccaccagacgatatcgctcctcgcgtgcc
ggacctgtacaccgacgttcatctgccgtccatggatccgccggagcacaccaaactgcgtgttccggtgcagcaggct
ctgctgccgggccgtctggtaggcaaagacgaggttgtgcgccgtattgcgaacgaactgatcgatacttttatcgaca
aaggcgaatgcgatctgctgcatgatttctcttataagctggcgctgtacctgattgtagacctgctgggtatccctaa
agaacgtgcggaggactaccaccgctggtccaactgtttcttccagctgttcacgccgaaggttcctgaacgtgcggat
gctcgtttctttgtaccgatgccggaggaagtgctgcgccagaactgggaaggtctggccgaagcaaatgactatctgc
gtgaagttgttgaaaacctggaccgtaatccgggcaacaacatgctgtctaacctgctgcaactgcgtgaaccggatgg
ctctcgtactatcactatctccgcgaacgtttgcaacgccctggaatttggcgcggcgggtcacgatacgaccgcaacc
ctgatcgctcacctgacttatttcgtgctgaccaccccggacctgaaggacactctgaccgaagatccatctctgattc
cggcagccatctctgaaaccctgcgtcgtcgttgcccggttgacggtctgttccgccgcaccctgagcgacgtagaact
gtgcggtcagaagatcgaatccggttctatcgtgtacctggacctgaccgccgctaacctggatccggacgttttcccg
gagccggagactttccgtctgaaccgcgataacattaaagaaatggtgtctttcggcttcggtcgtcacgtctgtgcag
gtcagtacctgagccgcatcgaagctaaagcagcatacgaagaactgatgcgtcgtattcctaatatgcgtctggcaga
tggcttcaaactggagtacatgccgagcgtggccaccactgttctgaaaggcctgccgctggtttgggataaaaacacc
ggttaa