LOCUS BBa_K525002 5538 bp DNA linear 25-Nov-2024 DEFINITION Fusion protein of SbpA, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac ACCESSION BBa_K525002 VERSION BBa_K525002.1 FEATURES Location/Qualifiers promoter 1..20 /label=T7 promoter misc_feature 73..3192 /label=K525401 misc_feature 20..40 /label=lac operator RBS 56..59 /label=RBS misc_feature 73..75 /label=ATG CDS 73..3192 /label=SbpA domain misc_feature 76..81 /label=NgoMIV misc_feature 3193..3198 /label=Freiburg scar CDS 3199..3939 /label=Reductase misc_feature 3199..5538 /label=K525560 misc_feature 3940..3945 /label=Freiburg scar CDS 3946..4258 /label=BisdA misc_feature 4258..4262 /label=Freiburg sacr CDS 4263..5538 /label=BisdB misc_feature 5530..5535 /label=AgeI misc_feature 5536..5538 /label=TAA misc_feature 53..64 /label=B0034 ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcctactag agaaagagga 61 gaaatactag agatggccgg cgctcaggtt aacgactaca acaagatctc cggctacgca 121 aaggaggctg ttcagtccct cgtcgaccag ggtgttatcc agggcgatac caacggcaac 181 ttcaacccac tgaacaccgt tactcgcgca caggctgctg agatcttcac caaggctctc 241 gaactggagg cagacggtga cgtcaacttc tccgatgtta agaagggcgc ttggtactac 301 aactctatcg cagctgttgt cgcaaacggc atcttcgagg gtgtgtccgc taacgagttc 361 gcaccaaaca agtcccttac ccgttccgag gctgcaaagg ttctcgttga cgctttcggc 421 ctggagggct ctgaatccct ctcccagttc gcagacgctt cccaggtcaa gggttgggca 481 aagtctgctc tggagaccgc agttgctaac ggcatcttca ctggctccga ggaaaacggt 541 aagcttaacc tcaagcctaa cgcagctatc acccgccagg atttcgcagt tgtcttcgct 601 cgtaccctgg acctcgcagt tgagaccgtt gacgcttccg tcaaggcaat caacaacact 661 accgtggagg ttaccttcga tgaagaggtt gacaacgtcg aggctctgaa gttcaagatc 721 gaaggccttg aggttaagaa cgcagctgtt aagcagacca acaagaaggt cgttgttctc 781 actaccgagg cacagaccgc tgacaaggaa tacgtcgtga ccctggatgg cgaggagatc 841 ggtaagttca agggcatcga agcagttgtt ccaaagtcca tcgtcctcaa gactaccaac 901 acccagggca aggttggtaa ccaggttacc ctgactgctg acgtcggcgt taaggcagct 961 ggcatcccag ttaccttcaa cgtcgacgca cctaccggct ctcttaacaa ggatgctgtg 1021 gttgaggttt acaccaacgc agagggcatc gcttcctact cctacactca gtacgcacca 1081 gacgctgacg atgtcaccgt ttacccaacc ggcgcacctc agctccgcgc tttcggtcca 1141 gtttactggg gcgtcgacaa catcctgacc atcgaagagg ttactaccgg caacaccctc 1201 gcaaacggtg ttaagaagac ctacaaggtc actttcaagg acccaaagac cggcgctgca 1261 ctgaccaaca agaagcttaa cgtgtccttc gttgagaaca ccaacgttgc tttcaacgca 1321 atctctaagg ctactgtcac caacccttcc tccggcctca ccgttacccc ataccagact 1381 accaccggcc tccaggaaga gatccaggtt accactgatt ccaacggcca ggcaaccttc 1441 gtcgtttctg gcaccaacac cgctgttact ccatacgtct tcgtggacgg ttcctcctcc 1501 gttctcggcg tttctaccgt caccggcacc aacaacgtta ctcaggcaac caccgctaac 1561 aagaagtggg aggcaaccga actgactgct accgcagctc aggttaagtt cgagggtgca 1621 cagcttaacc accagatcac cgtcgagcgt gacggcgaag aggaggcagc agctcagtac 1681 ggctccaagc tcaacggtcg cgaatacaag gttaaggttc tggataagga cggcaagcct 1741 tacgcaaacg gcctcgtcaa catcggtctg gacgaggtgc ttgatcgtaa cctcaacacc 1801 aactccaagg ctcagttcgc aaacgttact gacggcaccg ctctgaccct caacccaggc 1861 acctcccagc agggtaagat caagctgaac tctaagggcg aggcaactgt tgtcctttac 1921 ggcgctaagg gtgaagttgg caccccagtt gtctggatcg accagaacac ctcccagaac 1981 aaccagtccg gcgttctcga ggatggtgag cctttcttca aggcaccagt ttccaacttc 2041 cagtacgaac gcgtcatggg cgctgagttc accgtggagg gcaagacttc taaccagaac 2101 gttggtaccg acggcatcgc aaactacgct ttcgcactga ccaaccagtc cggcaagacc 2161 ctcactaacc tgatcggtaa gatgacctac gaaatccgta acacctccgg ccagccaatc 2221 gaggttaaga cccctatctc ctaccgcgac cagaacaacg tctcttccac tcttttcaag 2281 atcgaggaat acggctccat caccatctcc gctgatgtta tctctacctc caacgcagct 2341 ttcaccatcg agactgcacc aaacggtaag gctgcaaccg ttgtcgttac cccatccttc 2401 gttaccgcta acaacttcga cgcatccgct gacaacaacc tcaacggcgt cgtgactgat 2461 gcacgtgact tcaacaagca cgttaccgct cctgcactga ccgctacctt cggcaacaac 2521 aaggaggttg gtactaacta caccggcacc gtccagtact tcaacaagga caagaagacc 2581 atcactttca acggcaagaa cgcagttaag tacgctggtg aatctggcaa gacctacaag 2641 tacttcggcc tcggttccac cccaatctcc accgcagatg acttcatcac tcagctccag 2701 aacaacgctg caaacggctc cgttaccgtc acctacaagg ttgttgacaa cgaggtccag 2761 ttctacatcg tgtctttcaa caacgatggc gctgcaaacg ctgttaagcc agagcctacc 2821 gcagttgctc caactgacgg taccgtcgca ttcaccaaca cctccttcaa cgcttccgca 2881 aaccagacta tcaccgttac cgacgctgat cttaacgcag acgctaccgt tgcagaaact 2941 accaccgtca aggttacctc cggcactgtt gtctacaccc tcaccgctac cgagactggc 3001 gtgaacaccg gcgttttcac cgcaaccctg actgctgcac agctcgctgt tctgtctgac 3061 ggcgtcatca ccgcaaccta cgaggatgtt aagaacacca ctggctcctc cgttacccgc 3121 accgctaccg caactcttaa caccgtcgtt ggtcaggtta actacaacgc agcagctacc 3181 accgcatact ccaccggcgc tgattgggta acaggcaaag tcactaaagt gcagaactgg 3241 accgacgccc tgtttagtct caccgttcac gcccccgtgc ttccgtttac cgccgggcaa 3301 tttaccaagc ttggccttga aatcgacggc gaacgcgtcc agcgcgccta ctcctatgta 3361 aactcgcccg ataatcccga tctggagttt tacctggtca ccgtccccga tggcaaatta 3421 agcccacgac tggcggcact gaaaccaggc gatgaagtgc aggtggttag cgaagcggca 3481 ggattctttg tgctcgatga agtgccgcac tgcgaaacgc tatggatgct ggcaacgggc 3541 acagcgattg gcccttattt atcgattctg caactaggta aagatttaga tcgcttcaaa 3601 aatctggtcc tggtgcacgc cgcacgttat gccgccgact taagctattt gccactgatg 3661 caggaactgg aaaaacgcta cgaaggaaaa ctgcgcattc agacggtggt cagtcgggaa 3721 acggcagcgg ggtcgctcac cggacggata ccggcattaa ttgaaagtgg ggaactggaa 3781 agcacgattg gcctgccgat gaataaagaa accagccatg tgatgctgtg cggcaatcca 3841 cagatggtgc gcgatacaca acagttgctg aaagagaccc ggcagatgac gaaacattta 3901 cgtcgccgac cgggccatat gacagcggag cattactgga ccggccctca tatccaagtt 3961 acgactcgtg atggtgaaat tcgcgagctg gacgttgcag cttccggttt cctgatggaa 4021 gcgctgcgtg acgctaacat cgatggtgtg gaggcgatgt gtggcggttg ctgttcttgc 4081 gcgacttgcc acgtctacat tgacgcggct ccagccggta ccctgccgcc ggtgtctagc 4141 gatgaagaaa tgctgctgtc cggcctggtt tctaccccgg gccgctctcg tctgtcttgc 4201 cagatcccgg taaccgcaga actggacggc ctgaaactga ccatcccgcc ggatagcacc 4261 ggcaacccac aaactctgcc ggtatttcca gacctggata tcttcagccc ggaatacgct 4321 tgtaaccgcg aaaaatatgc agcgcgtgcg ctgcgcgatt acccgctgca cttttataaa 4381 ccgctgaacc tgtggattgt ctccaaacat aaagacgttc gtagcgcgct gttcaccccg 4441 caggtcttca gctccgtagc ttttggtctg ctgcctccac cagacgatat cgctcctcgc 4501 gtgccggacc tgtacaccga cgttcatctg ccgtccatgg atccgccgga gcacaccaaa 4561 ctgcgtgttc cggtgcagca ggctctgctg ccgggccgtc tggtaggcaa agacgaggtt 4621 gtgcgccgta ttgcgaacga actgatcgat acttttatcg acaaaggcga atgcgatctg 4681 ctgcatgatt tctcttataa gctggcgctg tacctgattg tagacctgct gggtatccct 4741 aaagaacgtg cggaggacta ccaccgctgg tccaactgtt tcttccagct gttcacgccg 4801 aaggttcctg aacgtgcgga tgctcgtttc tttgtaccga tgccggagga agtgctgcgc 4861 cagaactggg aaggtctggc cgaagcaaat gactatctgc gtgaagttgt tgaaaacctg 4921 gaccgtaatc cgggcaacaa catgctgtct aacctgctgc aactgcgtga accggatggc 4981 tctcgtacta tcactatctc cgcgaacgtt tgcaacgccc tggaatttgg cgcggcgggt 5041 cacgatacga ccgcaaccct gatcgctcac ctgacttatt tcgtgctgac caccccggac 5101 ctgaaggaca ctctgaccga agatccatct ctgattccgg cagccatctc tgaaaccctg 5161 cgtcgtcgtt gcccggttga cggtctgttc cgccgcaccc tgagcgacgt agaactgtgc 5221 ggtcagaaga tcgaatccgg ttctatcgtg tacctggacc tgaccgccgc taacctggat 5281 ccggacgttt tcccggagcc ggagactttc cgtctgaacc gcgataacat taaagaaatg 5341 gtgtctttcg gcttcggtcg tcacgtctgt gcaggtcagt acctgagccg catcgaagct 5401 aaagcagcat acgaagaact gatgcgtcgt attcctaata tgcgtctggc agatggcttc 5461 aaactggagt acatgccgag cgtggccacc actgttctga aaggcctgcc gctggtttgg 5521 gataaaaaca ccggttaa //