##gff-version 3
##sequence-region BBa_K525002 1 5538
BBa_K525002	.	CDS	4263	5538	.	+	0	ID=annotation2160933;Name=BisdB
BBa_K525002	.	non_covalent_binding_site	76	81	.	+	0	ID=annotation2160926;Name=NgoMIV
BBa_K525002	.	promoter	1	20	.	+	0	ID=annotation2160920;Name=T7 promoter
BBa_K525002	.	engineered_region	73	3192	.	+	0	ID=annotation2160921;Name=K525401
BBa_K525002	.	CDS	3946	4258	.	+	0	ID=annotation2160931;Name=BisdA
BBa_K525002	.	CDS	73	3192	.	+	0	ID=annotation2160925;Name=SbpA domain
BBa_K525002	.	engineered_region	3199	5538	.	+	0	ID=annotation2160929;Name=K525560
BBa_K525002	.	sequence_feature	4258	4262	.	+	0	ID=annotation2160932;Name=Freiburg sacr
BBa_K525002	.	CDS	3199	3939	.	+	0	ID=annotation2160928;Name=Reductase
BBa_K525002	.	non_covalent_binding_site	5530	5535	.	+	0	ID=annotation2160934;Name=AgeI
BBa_K525002	.	engineered_region	53	64	.	+	0	ID=annotation2161005;Name=B0034
BBa_K525002	.	sequence_feature	3193	3198	.	+	0	ID=annotation2160927;Name=Freiburg scar
BBa_K525002	.	operator	20	40	.	+	0	ID=annotation2160922;Name=lac operator
BBa_K525002	.	start_codon	73	75	.	+	0	ID=annotation2160924;Name=ATG
BBa_K525002	.	stop_codon	5536	5538	.	+	0	ID=annotation2160935;Name=TAA
BBa_K525002	.	ribosome_entry_site	56	59	.	+	0	ID=annotation2160923;Name=RBS
BBa_K525002	.	sequence_feature	3940	3945	.	+	0	ID=annotation2160930;Name=Freiburg scar
>BBa_K525002
taatacgactcactataggggaattgtgagcggataacaattcctactagagaaagaggagaaatactagagatggccg
gcgctcaggttaacgactacaacaagatctccggctacgcaaaggaggctgttcagtccctcgtcgaccagggtgttat
ccagggcgataccaacggcaacttcaacccactgaacaccgttactcgcgcacaggctgctgagatcttcaccaaggct
ctcgaactggaggcagacggtgacgtcaacttctccgatgttaagaagggcgcttggtactacaactctatcgcagctg
ttgtcgcaaacggcatcttcgagggtgtgtccgctaacgagttcgcaccaaacaagtcccttacccgttccgaggctgc
aaaggttctcgttgacgctttcggcctggagggctctgaatccctctcccagttcgcagacgcttcccaggtcaagggt
tgggcaaagtctgctctggagaccgcagttgctaacggcatcttcactggctccgaggaaaacggtaagcttaacctca
agcctaacgcagctatcacccgccaggatttcgcagttgtcttcgctcgtaccctggacctcgcagttgagaccgttga
cgcttccgtcaaggcaatcaacaacactaccgtggaggttaccttcgatgaagaggttgacaacgtcgaggctctgaag
ttcaagatcgaaggccttgaggttaagaacgcagctgttaagcagaccaacaagaaggtcgttgttctcactaccgagg
cacagaccgctgacaaggaatacgtcgtgaccctggatggcgaggagatcggtaagttcaagggcatcgaagcagttgt
tccaaagtccatcgtcctcaagactaccaacacccagggcaaggttggtaaccaggttaccctgactgctgacgtcggc
gttaaggcagctggcatcccagttaccttcaacgtcgacgcacctaccggctctcttaacaaggatgctgtggttgagg
tttacaccaacgcagagggcatcgcttcctactcctacactcagtacgcaccagacgctgacgatgtcaccgtttaccc
aaccggcgcacctcagctccgcgctttcggtccagtttactggggcgtcgacaacatcctgaccatcgaagaggttact
accggcaacaccctcgcaaacggtgttaagaagacctacaaggtcactttcaaggacccaaagaccggcgctgcactga
ccaacaagaagcttaacgtgtccttcgttgagaacaccaacgttgctttcaacgcaatctctaaggctactgtcaccaa
cccttcctccggcctcaccgttaccccataccagactaccaccggcctccaggaagagatccaggttaccactgattcc
aacggccaggcaaccttcgtcgtttctggcaccaacaccgctgttactccatacgtcttcgtggacggttcctcctccg
ttctcggcgtttctaccgtcaccggcaccaacaacgttactcaggcaaccaccgctaacaagaagtgggaggcaaccga
actgactgctaccgcagctcaggttaagttcgagggtgcacagcttaaccaccagatcaccgtcgagcgtgacggcgaa
gaggaggcagcagctcagtacggctccaagctcaacggtcgcgaatacaaggttaaggttctggataaggacggcaagc
cttacgcaaacggcctcgtcaacatcggtctggacgaggtgcttgatcgtaacctcaacaccaactccaaggctcagtt
cgcaaacgttactgacggcaccgctctgaccctcaacccaggcacctcccagcagggtaagatcaagctgaactctaag
ggcgaggcaactgttgtcctttacggcgctaagggtgaagttggcaccccagttgtctggatcgaccagaacacctccc
agaacaaccagtccggcgttctcgaggatggtgagcctttcttcaaggcaccagtttccaacttccagtacgaacgcgt
catgggcgctgagttcaccgtggagggcaagacttctaaccagaacgttggtaccgacggcatcgcaaactacgctttc
gcactgaccaaccagtccggcaagaccctcactaacctgatcggtaagatgacctacgaaatccgtaacacctccggcc
agccaatcgaggttaagacccctatctcctaccgcgaccagaacaacgtctcttccactcttttcaagatcgaggaata
cggctccatcaccatctccgctgatgttatctctacctccaacgcagctttcaccatcgagactgcaccaaacggtaag
gctgcaaccgttgtcgttaccccatccttcgttaccgctaacaacttcgacgcatccgctgacaacaacctcaacggcg
tcgtgactgatgcacgtgacttcaacaagcacgttaccgctcctgcactgaccgctaccttcggcaacaacaaggaggt
tggtactaactacaccggcaccgtccagtacttcaacaaggacaagaagaccatcactttcaacggcaagaacgcagtt
aagtacgctggtgaatctggcaagacctacaagtacttcggcctcggttccaccccaatctccaccgcagatgacttca
tcactcagctccagaacaacgctgcaaacggctccgttaccgtcacctacaaggttgttgacaacgaggtccagttcta
catcgtgtctttcaacaacgatggcgctgcaaacgctgttaagccagagcctaccgcagttgctccaactgacggtacc
gtcgcattcaccaacacctccttcaacgcttccgcaaaccagactatcaccgttaccgacgctgatcttaacgcagacg
ctaccgttgcagaaactaccaccgtcaaggttacctccggcactgttgtctacaccctcaccgctaccgagactggcgt
gaacaccggcgttttcaccgcaaccctgactgctgcacagctcgctgttctgtctgacggcgtcatcaccgcaacctac
gaggatgttaagaacaccactggctcctccgttacccgcaccgctaccgcaactcttaacaccgtcgttggtcaggtta
actacaacgcagcagctaccaccgcatactccaccggcgctgattgggtaacaggcaaagtcactaaagtgcagaactg
gaccgacgccctgtttagtctcaccgttcacgcccccgtgcttccgtttaccgccgggcaatttaccaagcttggcctt
gaaatcgacggcgaacgcgtccagcgcgcctactcctatgtaaactcgcccgataatcccgatctggagttttacctgg
tcaccgtccccgatggcaaattaagcccacgactggcggcactgaaaccaggcgatgaagtgcaggtggttagcgaagc
ggcaggattctttgtgctcgatgaagtgccgcactgcgaaacgctatggatgctggcaacgggcacagcgattggccct
tatttatcgattctgcaactaggtaaagatttagatcgcttcaaaaatctggtcctggtgcacgccgcacgttatgccg
ccgacttaagctatttgccactgatgcaggaactggaaaaacgctacgaaggaaaactgcgcattcagacggtggtcag
tcgggaaacggcagcggggtcgctcaccggacggataccggcattaattgaaagtggggaactggaaagcacgattggc
ctgccgatgaataaagaaaccagccatgtgatgctgtgcggcaatccacagatggtgcgcgatacacaacagttgctga
aagagacccggcagatgacgaaacatttacgtcgccgaccgggccatatgacagcggagcattactggaccggccctca
tatccaagttacgactcgtgatggtgaaattcgcgagctggacgttgcagcttccggtttcctgatggaagcgctgcgt
gacgctaacatcgatggtgtggaggcgatgtgtggcggttgctgttcttgcgcgacttgccacgtctacattgacgcgg
ctccagccggtaccctgccgccggtgtctagcgatgaagaaatgctgctgtccggcctggtttctaccccgggccgctc
tcgtctgtcttgccagatcccggtaaccgcagaactggacggcctgaaactgaccatcccgccggatagcaccggcaac
ccacaaactctgccggtatttccagacctggatatcttcagcccggaatacgcttgtaaccgcgaaaaatatgcagcgc
gtgcgctgcgcgattacccgctgcacttttataaaccgctgaacctgtggattgtctccaaacataaagacgttcgtag
cgcgctgttcaccccgcaggtcttcagctccgtagcttttggtctgctgcctccaccagacgatatcgctcctcgcgtg
ccggacctgtacaccgacgttcatctgccgtccatggatccgccggagcacaccaaactgcgtgttccggtgcagcagg
ctctgctgccgggccgtctggtaggcaaagacgaggttgtgcgccgtattgcgaacgaactgatcgatacttttatcga
caaaggcgaatgcgatctgctgcatgatttctcttataagctggcgctgtacctgattgtagacctgctgggtatccct
aaagaacgtgcggaggactaccaccgctggtccaactgtttcttccagctgttcacgccgaaggttcctgaacgtgcgg
atgctcgtttctttgtaccgatgccggaggaagtgctgcgccagaactgggaaggtctggccgaagcaaatgactatct
gcgtgaagttgttgaaaacctggaccgtaatccgggcaacaacatgctgtctaacctgctgcaactgcgtgaaccggat
ggctctcgtactatcactatctccgcgaacgtttgcaacgccctggaatttggcgcggcgggtcacgatacgaccgcaa
ccctgatcgctcacctgacttatttcgtgctgaccaccccggacctgaaggacactctgaccgaagatccatctctgat
tccggcagccatctctgaaaccctgcgtcgtcgttgcccggttgacggtctgttccgccgcaccctgagcgacgtagaa
ctgtgcggtcagaagatcgaatccggttctatcgtgtacctggacctgaccgccgctaacctggatccggacgttttcc
cggagccggagactttccgtctgaaccgcgataacattaaagaaatggtgtctttcggcttcggtcgtcacgtctgtgc
aggtcagtacctgagccgcatcgaagctaaagcagcatacgaagaactgatgcgtcgtattcctaatatgcgtctggca
gatggcttcaaactggagtacatgccgagcgtggccaccactgttctgaaaggcctgccgctggtttgggataaaaaca
ccggttaa