##gff-version 3
##sequence-region BBa_K525005 1 4425
BBa_K525005	.	CDS	73	2400	.	+	0	ID=annotation2160916;Name=SgsE domain
BBa_K525005	.	non_covalent_binding_site	76	81	.	+	0	ID=annotation2160914;Name=NgoMIV
BBa_K525005	.	non_covalent_binding_site	4417	4422	.	+	0	ID=annotation2160918;Name=AgeI
BBa_K525005	.	stop_codon	4423	4425	.	+	0	ID=annotation2160919;Name=TAA
BBa_K525005	.	engineered_region	53	64	.	+	0	ID=annotation2160911;Name=B0034
BBa_K525005	.	ribosome_entry_site	56	59	.	+	0	ID=annotation2160912;Name=RBS
BBa_K525005	.	promoter	1	20	.	+	0	ID=annotation2160909;Name=T7 promoter
BBa_K525005	.	sequence_feature	2401	2406	.	+	0	ID=annotation2160917;Name=Freiburg scar
BBa_K525005	.	start_codon	73	75	.	+	0	ID=annotation2160913;Name=ATG
BBa_K525005	.	CDS	2407	4425	.	+	0	ID=annotation2161007;Name=LigA domain
BBa_K525005	.	engineered_region	73	2400	.	+	0	ID=annotation2160915;Name=K525301
BBa_K525005	.	operator	20	44	.	+	0	ID=annotation2160910;Name=lac operator
BBa_K525005	.	engineered_region	2407	4425	.	+	0	ID=annotation2160943;Name=K525720
>BBa_K525005
taatacgactcactataggggaattgtgagcggataacaattcctactagagaaagaggagaaatactagagatggccg
gcgctaccaagctcgacaagatgcgccaggagctgaaggcagctgttgacgcaaaggatctcaagaaggctgaggaact
gtaccacaagatctcctacgagcttaagacccgtactgttatcctcgaccgcgtctacggccagtccacccgtgagctg
ctccgctccaccttcaaggcagacgctcaggcactgcgtgattctcttatctacgacatcaccgttgctatgaaggcac
gcgaagctcaggacgcagttaaggctggtaacctcgataaggcaaaggctgcactggaccaggtcaaccagtacgtttc
caaggttactgacgctttcaaggcagagctccagaaggctgcacaggatgctaaggcagcttacgaggcagctctgcca
ccaaaggtcgaatccgtgaccgcagttaacgctaagacccttgagatcaagttcaacaaggcagttgacgctgcaaccg
tcatcgacaacaagggcacttccgatacctctgacgacgttgttaaggctaccgcaatcaccctcaaggctatcgatga
ccaggtccctgtttccactgttaaggcatccctgtccgacgataagaagaccctcaagctggtcgtggacggcgctcag
ttcttcaccaagcgttacgttgttgacatcaagaacgtcaagacccttgatggtaaggacgttccagcatacactacca
ccatcgacaccactgattctgttcgcccatccgtcctctccttctcctacgctgacaacggcctgaccctcaaggttaa
gttctctgagcctctggcatccgttggcaccgtcaagctttacgacggtaccactgaaatctccgtgtccccaaagttc
accgctggcgatgacgagatgaccatcaacctcgcatcttcctccgttccagttaacaaggacctgaccctcaagatct
tcggcgctgtcgattacaacggtaacgttatcaaccctaacccagcagagctgactgttaagaagaccaccgtcgacat
caccaagccagttgttcagtccatcgaagctgtcaacactaagaccgtgaaggttaccttctctgagaagcttctctcc
gcacctaccatcaagatcggcggccagactgcttccgtttccgtcgactctactggtctggtttacaccgcaaccctcg
cttccgcactgtccaagggcgtttacgctgtcgaggtttccgattacaaggaccttgcaggcaactctggtgacgctta
cactaaggttgtccagctcaaggcagataacaccgctccaaagttcgtgtcctcccaggttgttaagatcaacggcgtc
gaacacctggttctcaccttcgacgaggaggttaccactggctccaacatcaccgtcgttcagtcttccgacaagtaca
tcgatgaaaacaacgttctgaaggcagtcggtgctgaccttaagaccacctccgacaacttcaagctctacctgccaac
tgatggcaagtccaagtctgtggcactcaacatctcctccctgcctaagggcacctacaccgttacccttccaaacggt
ctcgtttccgacctggctgacaacccatacgcagagcgtaagcagatcactttcgtccgcggctctgattccctcacca
ccaagcctgctctggacaaggactacgattccaacggcgttaaggcagacaacaacaacgagcttgttttcgctttcac
ccagaacctcgacgcatccgctctgaacctctctaacttcaacatcaacggtctgactgtcaccaaggcagttttcgat
ggcgacaccaagcacatccgtgttacccttgctccaggcgcaaacacttggactggtacccacgtcatcaccatctcca
acatcaagaacacttccggcctcgtgatggacaccgttaccgttaacgaatacatgaaggagaacgtcgctccaacctt
cactgcaaccctgacctccgctgatgttatccgcgttgacttctctgagcctgtcgcaaacgctaccatctcccgtgca
ctctccgctaacaacttcatcgttaaggttgacggcaacgtcgtgactgtttccaacgtttacgaagataacaacgcaa
ccaacctggtccagggttctaagggctacaagaccgtttaccttaagctccagtccccagttaccgacctgtccaagcc
aatcactctctccgctaccgacatcgtcgatgttaaccagaccggcgcaatcaccgacaacaacgttgtcggtaacaac
gtgtctgctactgttgttaacgtcgcaaagaccggcgaatcaatcgaacaacaactgacagaactgcgaacgacgcttc
gccatcatgaatatctttatcatgtgatggatgcgccggaaattcccgacgctgaatacgacaggctgatgcgcgaact
gcgcgagctggaaaccaaacatccagaactgattacgcctgattcgcctactcaacgtgtaggcgctgcgccgctggcg
gctttcagccagatacgccatgaagtaccaatgctgtcactggataacgtttttgatgaagaaagctttcttgctttca
acaaacgtgtgcaggaccgtctgaaaaacaacgagaaagtcacctggtgctgtgagctgaagctggatggtcttgccgt
cagtattctgtatgaaaatggcgttttagtcagtgccgcgacccgtggcgatggcaccaccggggaagatatcacgtct
aatgtgcgtactattcgcgccattccgctgaagctgcacggagagaatatcccggcgcgtctggaagtgcgtggtgaag
tgttcctgccgcaggcggggttcgaaaagattaacgaagatgcgcgacgcacgggcgggaaagtgtttgctaacccacg
taatgcggcagctggttcactgcgtcagcttgatccgcgtattacagcgaagcgaccgctcacttttttctgctatggc
gttggtgttctggaaggtggcgagctgccggatactcatcttggccgtttactgcaatttaaaaagtgggggttgccgg
tcagcgatcgggtaacgctttgtgaatcggcggaagaagtgctggcgttctatcacaaagtggaagaagaccgcccgac
gctgggctttgatatcgacggcgtggtgattaaggtcaactcactggcacagcaggagcagcttggctttgtcgcgcgt
gccccgcgctgggcggtagcgtttaaattcccggcgcaggagcagatgacctttgtgcgtgacgtcgagtttcaggttg
ggcgtactggcgcgattacgcctgttgcgcgtctggaacctgtccatgttgcaggcgtgctggtgagtaacgcaacctt
acacaatgcggatgaaatcgaacgtcttggtttacgcattggcgataaagtggtgattcgccgcgctggcgacgtgatc
ccgcaggtggttaacgtcgtgctttctgaacgcccggaagatacccgtgaggttgtattcccgacgcattgtccggtat
gtggttctgacgttgagcgtgtggaaggtgaagcggttgcccgctgtaccggcggcctgatttgcggtgcgcagcgtaa
agagtcgctgaaacactttgtttcccgccgtgcgatggatgttgacggaatgggcgacaaaatcatcgatcagctggtt
gaaaaagaatatgtccatactccggcagatctgttcaaactcaccgcaggcaaactgaccggactggagcgtatggggc
caaaatcggcacaaaacgtggttaacgcgctggaaaaagcgaaagaaaccacctttgctcgcttcctctatgcacttgg
catccgtgaagtcggcgaggccaccgcagcaggtctggcggcatatttcggcacgctggaagcgctggaagccgcttcg
attgaagagctgcaaaaggtgcctgatgttggcattgtcgttgcatcccacgttcacaacttctttgccgaagaaagca
accgcaatgtcatcagcgagctgttggcggaaggtgttcactggcctgcgccgatcgttatcaacgcggaagagattga
cagcccgtttgctggtaaaaccgtggtgcttacgggcagcttaagccagatgtcgcgtgatgacgctaaagctcgactg
gtcgaactgggcgcgaaagtcgcgggcagcgtgtcgaagaaaaccgatctggtgatagcgggtgaagcggcaggatcta
aactggcgaaggcgcaggaactgggcattgaagtcatcgacgaagcggaaatgctgcgtttgctgggtagcaccggttaa
a