LOCUS BBa_K525006 5217 bp DNA linear 01-Jun-2024 DEFINITION Fusion Protein of S-Layer SbpA and LigA ACCESSION BBa_K525006 VERSION BBa_K525006.1 FEATURES Location/Qualifiers misc_feature 5214..5217 /label=TAA misc_feature 73..3193 /label=K525401 promoter 1..20 /label=T7 Promotor misc_feature 20..44 /label=lac operator misc_feature 53..64 /label=B0034 CDS 73..3192 /label=SbpA domain misc_feature 73..75 /label=ATG misc_feature 3193..3198 /label=Freiburg Scar misc_feature 3194..5217 /label=K525720 CDS 3199..5208 /label=LigA domain misc_feature 76..82 /label=NgoMIV misc_feature 5209..5215 /label=AgeI RBS 56..59 /label=RBS ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcctactag agaaagagga 61 gaaatactag agatggccgg cgctcaggtt aacgactaca acaagatctc cggctacgca 121 aaggaggctg ttcagtccct cgtcgaccag ggtgttatcc agggcgatac caacggcaac 181 ttcaacccac tgaacaccgt tactcgcgca caggctgctg agatcttcac caaggctctc 241 gaactggagg cagacggtga cgtcaacttc tccgatgtta agaagggcgc ttggtactac 301 aactctatcg cagctgttgt cgcaaacggc atcttcgagg gtgtgtccgc taacgagttc 361 gcaccaaaca agtcccttac ccgttccgag gctgcaaagg ttctcgttga cgctttcggc 421 ctggagggct ctgaatccct ctcccagttc gcagacgctt cccaggtcaa gggttgggca 481 aagtctgctc tggagaccgc agttgctaac ggcatcttca ctggctccga ggaaaacggt 541 aagcttaacc tcaagcctaa cgcagctatc acccgccagg atttcgcagt tgtcttcgct 601 cgtaccctgg acctcgcagt tgagaccgtt gacgcttccg tcaaggcaat caacaacact 661 accgtggagg ttaccttcga tgaagaggtt gacaacgtcg aggctctgaa gttcaagatc 721 gaaggccttg aggttaagaa cgcagctgtt aagcagacca acaagaaggt cgttgttctc 781 actaccgagg cacagaccgc tgacaaggaa tacgtcgtga ccctggatgg cgaggagatc 841 ggtaagttca agggcatcga agcagttgtt ccaaagtcca tcgtcctcaa gactaccaac 901 acccagggca aggttggtaa ccaggttacc ctgactgctg acgtcggcgt taaggcagct 961 ggcatcccag ttaccttcaa cgtcgacgca cctaccggct ctcttaacaa ggatgctgtg 1021 gttgaggttt acaccaacgc agagggcatc gcttcctact cctacactca gtacgcacca 1081 gacgctgacg atgtcaccgt ttacccaacc ggcgcacctc agctccgcgc tttcggtcca 1141 gtttactggg gcgtcgacaa catcctgacc atcgaagagg ttactaccgg caacaccctc 1201 gcaaacggtg ttaagaagac ctacaaggtc actttcaagg acccaaagac cggcgctgca 1261 ctgaccaaca agaagcttaa cgtgtccttc gttgagaaca ccaacgttgc tttcaacgca 1321 atctctaagg ctactgtcac caacccttcc tccggcctca ccgttacccc ataccagact 1381 accaccggcc tccaggaaga gatccaggtt accactgatt ccaacggcca ggcaaccttc 1441 gtcgtttctg gcaccaacac cgctgttact ccatacgtct tcgtggacgg ttcctcctcc 1501 gttctcggcg tttctaccgt caccggcacc aacaacgtta ctcaggcaac caccgctaac 1561 aagaagtggg aggcaaccga actgactgct accgcagctc aggttaagtt cgagggtgca 1621 cagcttaacc accagatcac cgtcgagcgt gacggcgaag aggaggcagc agctcagtac 1681 ggctccaagc tcaacggtcg cgaatacaag gttaaggttc tggataagga cggcaagcct 1741 tacgcaaacg gcctcgtcaa catcggtctg gacgaggtgc ttgatcgtaa cctcaacacc 1801 aactccaagg ctcagttcgc aaacgttact gacggcaccg ctctgaccct caacccaggc 1861 acctcccagc agggtaagat caagctgaac tctaagggcg aggcaactgt tgtcctttac 1921 ggcgctaagg gtgaagttgg caccccagtt gtctggatcg accagaacac ctcccagaac 1981 aaccagtccg gcgttctcga ggatggtgag cctttcttca aggcaccagt ttccaacttc 2041 cagtacgaac gcgtcatggg cgctgagttc accgtggagg gcaagacttc taaccagaac 2101 gttggtaccg acggcatcgc aaactacgct ttcgcactga ccaaccagtc cggcaagacc 2161 ctcactaacc tgatcggtaa gatgacctac gaaatccgta acacctccgg ccagccaatc 2221 gaggttaaga cccctatctc ctaccgcgac cagaacaacg tctcttccac tcttttcaag 2281 atcgaggaat acggctccat caccatctcc gctgatgtta tctctacctc caacgcagct 2341 ttcaccatcg agactgcacc aaacggtaag gctgcaaccg ttgtcgttac cccatccttc 2401 gttaccgcta acaacttcga cgcatccgct gacaacaacc tcaacggcgt cgtgactgat 2461 gcacgtgact tcaacaagca cgttaccgct cctgcactga ccgctacctt cggcaacaac 2521 aaggaggttg gtactaacta caccggcacc gtccagtact tcaacaagga caagaagacc 2581 atcactttca acggcaagaa cgcagttaag tacgctggtg aatctggcaa gacctacaag 2641 tacttcggcc tcggttccac cccaatctcc accgcagatg acttcatcac tcagctccag 2701 aacaacgctg caaacggctc cgttaccgtc acctacaagg ttgttgacaa cgaggtccag 2761 ttctacatcg tgtctttcaa caacgatggc gctgcaaacg ctgttaagcc agagcctacc 2821 gcagttgctc caactgacgg taccgtcgca ttcaccaaca cctccttcaa cgcttccgca 2881 aaccagacta tcaccgttac cgacgctgat cttaacgcag acgctaccgt tgcagaaact 2941 accaccgtca aggttacctc cggcactgtt gtctacaccc tcaccgctac cgagactggc 3001 gtgaacaccg gcgttttcac cgcaaccctg actgctgcac agctcgctgt tctgtctgac 3061 ggcgtcatca ccgcaaccta cgaggatgtt aagaacacca ctggctcctc cgttacccgc 3121 accgctaccg caactcttaa caccgtcgtt ggtcaggtta actacaacgc agcagctacc 3181 accgcatact ccaccggcga atcaatcgaa caacaactga cagaactgcg aacgacgctt 3241 cgccatcatg aatatcttta tcatgtgatg gatgcgccgg aaattcccga cgctgaatac 3301 gacaggctga tgcgcgaact gcgcgagctg gaaaccaaac atccagaact gattacgcct 3361 gattcgccta ctcaacgtgt aggcgctgcg ccgctggcgg ctttcagcca gatacgccat 3421 gaagtaccaa tgctgtcact ggataacgtt tttgatgaag aaagctttct tgctttcaac 3481 aaacgtgtgc aggaccgtct gaaaaacaac gagaaagtca cctggtgctg tgagctgaag 3541 ctggatggtc ttgccgtcag tattctgtat gaaaatggcg ttttagtcag tgccgcgacc 3601 cgtggcgatg gcaccaccgg ggaagatatc acgtctaatg tgcgtactat tcgcgccatt 3661 ccgctgaagc tgcacggaga gaatatcccg gcgcgtctgg aagtgcgtgg tgaagtgttc 3721 ctgccgcagg cggggttcga aaagattaac gaagatgcgc gacgcacggg cgggaaagtg 3781 tttgctaacc cacgtaatgc ggcagctggt tcactgcgtc agcttgatcc gcgtattaca 3841 gcgaagcgac cgctcacttt tttctgctat ggcgttggtg ttctggaagg tggcgagctg 3901 ccggatactc atcttggccg tttactgcaa tttaaaaagt gggggttgcc ggtcagcgat 3961 cgggtaacgc tttgtgaatc ggcggaagaa gtgctggcgt tctatcacaa agtggaagaa 4021 gaccgcccga cgctgggctt tgatatcgac ggcgtggtga ttaaggtcaa ctcactggca 4081 cagcaggagc agcttggctt tgtcgcgcgt gccccgcgct gggcggtagc gtttaaattc 4141 ccggcgcagg agcagatgac ctttgtgcgt gacgtcgagt ttcaggttgg gcgtactggc 4201 gcgattacgc ctgttgcgcg tctggaacct gtccatgttg caggcgtgct ggtgagtaac 4261 gcaaccttac acaatgcgga tgaaatcgaa cgtcttggtt tacgcattgg cgataaagtg 4321 gtgattcgcc gcgctggcga cgtgatcccg caggtggtta acgtcgtgct ttctgaacgc 4381 ccggaagata cccgtgaggt tgtattcccg acgcattgtc cggtatgtgg ttctgacgtt 4441 gagcgtgtgg aaggtgaagc ggttgcccgc tgtaccggcg gcctgatttg cggtgcgcag 4501 cgtaaagagt cgctgaaaca ctttgtttcc cgccgtgcga tggatgttga cggaatgggc 4561 gacaaaatca tcgatcagct ggttgaaaaa gaatatgtcc atactccggc agatctgttc 4621 aaactcaccg caggcaaact gaccggactg gagcgtatgg ggccaaaatc ggcacaaaac 4681 gtggttaacg cgctggaaaa agcgaaagaa accacctttg ctcgcttcct ctatgcactt 4741 ggcatccgtg aagtcggcga ggccaccgca gcaggtctgg cggcatattt cggcacgctg 4801 gaagcgctgg aagccgcttc gattgaagag ctgcaaaagg tgcctgatgt tggcattgtc 4861 gttgcatccc acgttcacaa cttctttgcc gaagaaagca accgcaatgt catcagcgag 4921 ctgttggcgg aaggtgttca ctggcctgcg ccgatcgtta tcaacgcgga agagattgac 4981 agcccgtttg ctggtaaaac cgtggtgctt acgggcagct taagccagat gtcgcgtgat 5041 gacgctaaag ctcgactggt cgaactgggc gcgaaagtcg cgggcagcgt gtcgaagaaa 5101 accgatctgg tgatagcggg tgaagcggca ggatctaaac tggcgaaggc gcaggaactg 5161 ggcattgaag tcatcgacga agcggaaatg ctgcgtttgc tgggtagcac cggttaa //