##gff-version 3
##sequence-region BBa_K525007 1 3999
BBa_K525007	.	stop_codon	3997	3999	.	+	0	ID=annotation2161176;Name=TAA
BBa_K525007	.	engineered_region	2407	3999	.	+	0	ID=annotation2161171;Name=K525515
BBa_K525007	.	start_codon	73	75	.	+	0	ID=annotation2161166;Name=ATG
BBa_K525007	.	operator	20	44	.	+	0	ID=annotation2161163;Name=lac operator
BBa_K525007	.	engineered_region	73	2400	.	+	0	ID=annotation2161168;Name=K525301
BBa_K525007	.	non_covalent_binding_site	76	82	.	+	0	ID=annotation2161169;Name=NgoMIV
BBa_K525007	.	promoter	1	20	.	+	0	ID=annotation2161162;Name=T7
BBa_K525007	.	sequence_feature	2401	2406	.	+	0	ID=annotation2161170;Name=Freiburg Scar
BBa_K525007	.	non_covalent_binding_site	3991	3996	.	+	0	ID=annotation2161175;Name=AgeI
BBa_K525007	.	CDS	2725	3999	.	+	0	ID=annotation2161174;Name=BisdB
BBa_K525007	.	CDS	73	2400	.	+	0	ID=annotation2161167;Name=SgsE domain
BBa_K525007	.	CDS	2407	2718	.	+	0	ID=annotation2161172;Name=BisdA
BBa_K525007	.	sequence_feature	2719	2724	.	+	0	ID=annotation2161173;Name=Freiburg Scar
BBa_K525007	.	ribosome_entry_site	56	59	.	+	0	ID=annotation2161165;Name=RBS
BBa_K525007	.	engineered_region	53	64	.	+	0	ID=annotation2161164;Name=B0034
>BBa_K525007
taatacgactcactataggggaattgtgagcggataacaattcctactagagaaagaggagaaatactagagatggccg
gcgctaccaagctcgacaagatgcgccaggagctgaaggcagctgttgacgcaaaggatctcaagaaggctgaggaact
gtaccacaagatctcctacgagcttaagacccgtactgttatcctcgaccgcgtctacggccagtccacccgtgagctg
ctccgctccaccttcaaggcagacgctcaggcactgcgtgattctcttatctacgacatcaccgttgctatgaaggcac
gcgaagctcaggacgcagttaaggctggtaacctcgataaggcaaaggctgcactggaccaggtcaaccagtacgtttc
caaggttactgacgctttcaaggcagagctccagaaggctgcacaggatgctaaggcagcttacgaggcagctctgcca
ccaaaggtcgaatccgtgaccgcagttaacgctaagacccttgagatcaagttcaacaaggcagttgacgctgcaaccg
tcatcgacaacaagggcacttccgatacctctgacgacgttgttaaggctaccgcaatcaccctcaaggctatcgatga
ccaggtccctgtttccactgttaaggcatccctgtccgacgataagaagaccctcaagctggtcgtggacggcgctcag
ttcttcaccaagcgttacgttgttgacatcaagaacgtcaagacccttgatggtaaggacgttccagcatacactacca
ccatcgacaccactgattctgttcgcccatccgtcctctccttctcctacgctgacaacggcctgaccctcaaggttaa
gttctctgagcctctggcatccgttggcaccgtcaagctttacgacggtaccactgaaatctccgtgtccccaaagttc
accgctggcgatgacgagatgaccatcaacctcgcatcttcctccgttccagttaacaaggacctgaccctcaagatct
tcggcgctgtcgattacaacggtaacgttatcaaccctaacccagcagagctgactgttaagaagaccaccgtcgacat
caccaagccagttgttcagtccatcgaagctgtcaacactaagaccgtgaaggttaccttctctgagaagcttctctcc
gcacctaccatcaagatcggcggccagactgcttccgtttccgtcgactctactggtctggtttacaccgcaaccctcg
cttccgcactgtccaagggcgtttacgctgtcgaggtttccgattacaaggaccttgcaggcaactctggtgacgctta
cactaaggttgtccagctcaaggcagataacaccgctccaaagttcgtgtcctcccaggttgttaagatcaacggcgtc
gaacacctggttctcaccttcgacgaggaggttaccactggctccaacatcaccgtcgttcagtcttccgacaagtaca
tcgatgaaaacaacgttctgaaggcagtcggtgctgaccttaagaccacctccgacaacttcaagctctacctgccaac
tgatggcaagtccaagtctgtggcactcaacatctcctccctgcctaagggcacctacaccgttacccttccaaacggt
ctcgtttccgacctggctgacaacccatacgcagagcgtaagcagatcactttcgtccgcggctctgattccctcacca
ccaagcctgctctggacaaggactacgattccaacggcgttaaggcagacaacaacaacgagcttgttttcgctttcac
ccagaacctcgacgcatccgctctgaacctctctaacttcaacatcaacggtctgactgtcaccaaggcagttttcgat
ggcgacaccaagcacatccgtgttacccttgctccaggcgcaaacacttggactggtacccacgtcatcaccatctcca
acatcaagaacacttccggcctcgtgatggacaccgttaccgttaacgaatacatgaaggagaacgtcgctccaacctt
cactgcaaccctgacctccgctgatgttatccgcgttgacttctctgagcctgtcgcaaacgctaccatctcccgtgca
ctctccgctaacaacttcatcgttaaggttgacggcaacgtcgtgactgtttccaacgtttacgaagataacaacgcaa
ccaacctggtccagggttctaagggctacaagaccgtttaccttaagctccagtccccagttaccgacctgtccaagcc
aatcactctctccgctaccgacatcgtcgatgttaaccagaccggcgcaatcaccgacaacaacgttgtcggtaacaac
gtgtctgctactgttgttaacgtcgcaaagaccggccctcatatccaagttacgactcgtgatggtgaaattcgcgagc
tggacgttgcagcttccggtttcctgatggaagcgctgcgtgacgctaacatcgatggtgtggaggcgatgtgtggcgg
ttgctgttcttgcgcgacttgccacgtctacattgacgcggctccagccggtaccctgccgccggtgtctagcgatgaa
gaaatgctgctgtccggcctggtttctaccccgggccgctctcgtctgtcttgccagatcccggtaaccgcagaactgg
acggcctgaaactgaccatcccgccggatagcaccggcaacccacaaactctgccggtatttccagacctggatatctt
cagcccggaatacgcttgtaaccgcgaaaaatatgcagcgcgtgcgctgcgcgattacccgctgcacttttataaaccg
ctgaacctgtggattgtctccaaacataaagacgttcgtagcgcgctgttcaccccgcaggtcttcagctccgtagctt
ttggtctgctgcctccaccagacgatatcgctcctcgcgtgccggacctgtacaccgacgttcatctgccgtccatgga
tccgccggagcacaccaaactgcgtgttccggtgcagcaggctctgctgccgggccgtctggtaggcaaagacgaggtt
gtgcgccgtattgcgaacgaactgatcgatacttttatcgacaaaggcgaatgcgatctgctgcatgatttctcttata
agctggcgctgtacctgattgtagacctgctgggtatccctaaagaacgtgcggaggactaccaccgctggtccaactg
tttcttccagctgttcacgccgaaggttcctgaacgtgcggatgctcgtttctttgtaccgatgccggaggaagtgctg
cgccagaactgggaaggtctggccgaagcaaatgactatctgcgtgaagttgttgaaaacctggaccgtaatccgggca
acaacatgctgtctaacctgctgcaactgcgtgaaccggatggctctcgtactatcactatctccgcgaacgtttgcaa
cgccctggaatttggcgcggcgggtcacgatacgaccgcaaccctgatcgctcacctgacttatttcgtgctgaccacc
ccggacctgaaggacactctgaccgaagatccatctctgattccggcagccatctctgaaaccctgcgtcgtcgttgcc
cggttgacggtctgttccgccgcaccctgagcgacgtagaactgtgcggtcagaagatcgaatccggttctatcgtgta
cctggacctgaccgccgctaacctggatccggacgttttcccggagccggagactttccgtctgaaccgcgataacatt
aaagaaatggtgtctttcggcttcggtcgtcacgtctgtgcaggtcagtacctgagccgcatcgaagctaaagcagcat
acgaagaactgatgcgtcgtattcctaatatgcgtctggcagatggcttcaaactggagtacatgccgagcgtggccac
cactgttctgaaaggcctgccgctggtttgggataaaaacaccggttaa