LOCUS BBa_K525008 4791 bp DNA linear 22-Nov-2024 DEFINITION Fusion protein of S-Layer SbpA with T7/lac and BisdA and BisdB ACCESSION BBa_K525008 VERSION BBa_K525008.1 FEATURES Location/Qualifiers promoter 1..20 /label=T7 misc_feature 20..44 /label=lac operator misc_feature 53..64 /label=B0034 RBS 56..59 /label=RBS misc_feature 73..3192 /label=K525401 misc_feature 73..75 /label=ATG CDS 73..3192 /label=SbpA domain misc_feature 76..82 /label=NgoMIV misc_feature 3193..3198 /label=Freiburg Scar CDS 3199..3510 /label=BisdA misc_feature 3511..3516 /label=Freiburg Scar CDS 3517..4791 /label=BisdB misc_feature 4789..4791 /label=TAA misc_feature 3199..4791 /label=K525515 misc_feature 4783..4788 /label=AgeI ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcctactag agaaagagga 61 gaaatactag agatggccgg cgctcaggtt aacgactaca acaagatctc cggctacgca 121 aaggaggctg ttcagtccct cgtcgaccag ggtgttatcc agggcgatac caacggcaac 181 ttcaacccac tgaacaccgt tactcgcgca caggctgctg agatcttcac caaggctctc 241 gaactggagg cagacggtga cgtcaacttc tccgatgtta agaagggcgc ttggtactac 301 aactctatcg cagctgttgt cgcaaacggc atcttcgagg gtgtgtccgc taacgagttc 361 gcaccaaaca agtcccttac ccgttccgag gctgcaaagg ttctcgttga cgctttcggc 421 ctggagggct ctgaatccct ctcccagttc gcagacgctt cccaggtcaa gggttgggca 481 aagtctgctc tggagaccgc agttgctaac ggcatcttca ctggctccga ggaaaacggt 541 aagcttaacc tcaagcctaa cgcagctatc acccgccagg atttcgcagt tgtcttcgct 601 cgtaccctgg acctcgcagt tgagaccgtt gacgcttccg tcaaggcaat caacaacact 661 accgtggagg ttaccttcga tgaagaggtt gacaacgtcg aggctctgaa gttcaagatc 721 gaaggccttg aggttaagaa cgcagctgtt aagcagacca acaagaaggt cgttgttctc 781 actaccgagg cacagaccgc tgacaaggaa tacgtcgtga ccctggatgg cgaggagatc 841 ggtaagttca agggcatcga agcagttgtt ccaaagtcca tcgtcctcaa gactaccaac 901 acccagggca aggttggtaa ccaggttacc ctgactgctg acgtcggcgt taaggcagct 961 ggcatcccag ttaccttcaa cgtcgacgca cctaccggct ctcttaacaa ggatgctgtg 1021 gttgaggttt acaccaacgc agagggcatc gcttcctact cctacactca gtacgcacca 1081 gacgctgacg atgtcaccgt ttacccaacc ggcgcacctc agctccgcgc tttcggtcca 1141 gtttactggg gcgtcgacaa catcctgacc atcgaagagg ttactaccgg caacaccctc 1201 gcaaacggtg ttaagaagac ctacaaggtc actttcaagg acccaaagac cggcgctgca 1261 ctgaccaaca agaagcttaa cgtgtccttc gttgagaaca ccaacgttgc tttcaacgca 1321 atctctaagg ctactgtcac caacccttcc tccggcctca ccgttacccc ataccagact 1381 accaccggcc tccaggaaga gatccaggtt accactgatt ccaacggcca ggcaaccttc 1441 gtcgtttctg gcaccaacac cgctgttact ccatacgtct tcgtggacgg ttcctcctcc 1501 gttctcggcg tttctaccgt caccggcacc aacaacgtta ctcaggcaac caccgctaac 1561 aagaagtggg aggcaaccga actgactgct accgcagctc aggttaagtt cgagggtgca 1621 cagcttaacc accagatcac cgtcgagcgt gacggcgaag aggaggcagc agctcagtac 1681 ggctccaagc tcaacggtcg cgaatacaag gttaaggttc tggataagga cggcaagcct 1741 tacgcaaacg gcctcgtcaa catcggtctg gacgaggtgc ttgatcgtaa cctcaacacc 1801 aactccaagg ctcagttcgc aaacgttact gacggcaccg ctctgaccct caacccaggc 1861 acctcccagc agggtaagat caagctgaac tctaagggcg aggcaactgt tgtcctttac 1921 ggcgctaagg gtgaagttgg caccccagtt gtctggatcg accagaacac ctcccagaac 1981 aaccagtccg gcgttctcga ggatggtgag cctttcttca aggcaccagt ttccaacttc 2041 cagtacgaac gcgtcatggg cgctgagttc accgtggagg gcaagacttc taaccagaac 2101 gttggtaccg acggcatcgc aaactacgct ttcgcactga ccaaccagtc cggcaagacc 2161 ctcactaacc tgatcggtaa gatgacctac gaaatccgta acacctccgg ccagccaatc 2221 gaggttaaga cccctatctc ctaccgcgac cagaacaacg tctcttccac tcttttcaag 2281 atcgaggaat acggctccat caccatctcc gctgatgtta tctctacctc caacgcagct 2341 ttcaccatcg agactgcacc aaacggtaag gctgcaaccg ttgtcgttac cccatccttc 2401 gttaccgcta acaacttcga cgcatccgct gacaacaacc tcaacggcgt cgtgactgat 2461 gcacgtgact tcaacaagca cgttaccgct cctgcactga ccgctacctt cggcaacaac 2521 aaggaggttg gtactaacta caccggcacc gtccagtact tcaacaagga caagaagacc 2581 atcactttca acggcaagaa cgcagttaag tacgctggtg aatctggcaa gacctacaag 2641 tacttcggcc tcggttccac cccaatctcc accgcagatg acttcatcac tcagctccag 2701 aacaacgctg caaacggctc cgttaccgtc acctacaagg ttgttgacaa cgaggtccag 2761 ttctacatcg tgtctttcaa caacgatggc gctgcaaacg ctgttaagcc agagcctacc 2821 gcagttgctc caactgacgg taccgtcgca ttcaccaaca cctccttcaa cgcttccgca 2881 aaccagacta tcaccgttac cgacgctgat cttaacgcag acgctaccgt tgcagaaact 2941 accaccgtca aggttacctc cggcactgtt gtctacaccc tcaccgctac cgagactggc 3001 gtgaacaccg gcgttttcac cgcaaccctg actgctgcac agctcgctgt tctgtctgac 3061 ggcgtcatca ccgcaaccta cgaggatgtt aagaacacca ctggctcctc cgttacccgc 3121 accgctaccg caactcttaa caccgtcgtt ggtcaggtta actacaacgc agcagctacc 3181 accgcatact ccaccggccc tcatatccaa gttacgactc gtgatggtga aattcgcgag 3241 ctggacgttg cagcttccgg tttcctgatg gaagcgctgc gtgacgctaa catcgatggt 3301 gtggaggcga tgtgtggcgg ttgctgttct tgcgcgactt gccacgtcta cattgacgcg 3361 gctccagccg gtaccctgcc gccggtgtct agcgatgaag aaatgctgct gtccggcctg 3421 gtttctaccc cgggccgctc tcgtctgtct tgccagatcc cggtaaccgc agaactggac 3481 ggcctgaaac tgaccatccc gccggatagc accggcaacc cacaaactct gccggtattt 3541 ccagacctgg atatcttcag cccggaatac gcttgtaacc gcgaaaaata tgcagcgcgt 3601 gcgctgcgcg attacccgct gcacttttat aaaccgctga acctgtggat tgtctccaaa 3661 cataaagacg ttcgtagcgc gctgttcacc ccgcaggtct tcagctccgt agcttttggt 3721 ctgctgcctc caccagacga tatcgctcct cgcgtgccgg acctgtacac cgacgttcat 3781 ctgccgtcca tggatccgcc ggagcacacc aaactgcgtg ttccggtgca gcaggctctg 3841 ctgccgggcc gtctggtagg caaagacgag gttgtgcgcc gtattgcgaa cgaactgatc 3901 gatactttta tcgacaaagg cgaatgcgat ctgctgcatg atttctctta taagctggcg 3961 ctgtacctga ttgtagacct gctgggtatc cctaaagaac gtgcggagga ctaccaccgc 4021 tggtccaact gtttcttcca gctgttcacg ccgaaggttc ctgaacgtgc ggatgctcgt 4081 ttctttgtac cgatgccgga ggaagtgctg cgccagaact gggaaggtct ggccgaagca 4141 aatgactatc tgcgtgaagt tgttgaaaac ctggaccgta atccgggcaa caacatgctg 4201 tctaacctgc tgcaactgcg tgaaccggat ggctctcgta ctatcactat ctccgcgaac 4261 gtttgcaacg ccctggaatt tggcgcggcg ggtcacgata cgaccgcaac cctgatcgct 4321 cacctgactt atttcgtgct gaccaccccg gacctgaagg acactctgac cgaagatcca 4381 tctctgattc cggcagccat ctctgaaacc ctgcgtcgtc gttgcccggt tgacggtctg 4441 ttccgccgca ccctgagcga cgtagaactg tgcggtcaga agatcgaatc cggttctatc 4501 gtgtacctgg acctgaccgc cgctaacctg gatccggacg ttttcccgga gccggagact 4561 ttccgtctga accgcgataa cattaaagaa atggtgtctt tcggcttcgg tcgtcacgtc 4621 tgtgcaggtc agtacctgag ccgcatcgaa gctaaagcag catacgaaga actgatgcgt 4681 cgtattccta atatgcgtct ggcagatggc ttcaaactgg agtacatgcc gagcgtggcc 4741 accactgttc tgaaaggcct gccgctggtt tgggataaaa acaccggtta a //