BBa_K525009 1 BBa_K525009 Fusion Protein of S-Layer SgsE and LigA with His-Tag 2011-10-24T11:00:00Z 2015-05-08T01:12:34Z SgsE was synthesized, LigA with His-Tag created by PCR. LigA is the natural ligase A of E. coli. Fusion Protein of S-Layer SgsE and LigA with His-Tag false false _690_ 0 8718 9 Not in stock false This part was assembled by Freiburg Standard Assembly (RFC 25) false Manuel Wittchen annotation2160510 1 lac operator range2160510 1 20 44 annotation2160516 1 K525740 range2160516 1 2410 4434 annotation2160514 1 NogMIV range2160514 1 76 82 annotation2160509 1 K525303 range2160509 1 1 2409 annotation2160515 1 Freiburg scar range2160515 1 2401 2407 annotation2160508 1 T7 promotor range2160508 1 1 20 annotation2160518 1 His-Tag range2160518 1 4417 4434 annotation2160513 1 SgsE domain range2160513 1 73 2409 annotation2160511 1 B0034 range2160511 1 53 64 annotation2160512 1 ATG range2160512 1 73 75 annotation2160517 1 LigA domain range2160517 1 2407 4416 BBa_K525009_sequence 1 taatacgactcactataggggaattgtgagcggataacaattcctactagagaaagaggagaaatactagagatggccggcgctaccaagctcgacaagatgcgccaggagctgaaggcagctgttgacgcaaaggatctcaagaaggctgaggaactgtaccacaagatctcctacgagcttaagacccgtactgttatcctcgaccgcgtctacggccagtccacccgtgagctgctccgctccaccttcaaggcagacgctcaggcactgcgtgattctcttatctacgacatcaccgttgctatgaaggcacgcgaagctcaggacgcagttaaggctggtaacctcgataaggcaaaggctgcactggaccaggtcaaccagtacgtttccaaggttactgacgctttcaaggcagagctccagaaggctgcacaggatgctaaggcagcttacgaggcagctctgccaccaaaggtcgaatccgtgaccgcagttaacgctaagacccttgagatcaagttcaacaaggcagttgacgctgcaaccgtcatcgacaacaagggcacttccgatacctctgacgacgttgttaaggctaccgcaatcaccctcaaggctatcgatgaccaggtccctgtttccactgttaaggcatccctgtccgacgataagaagaccctcaagctggtcgtggacggcgctcagttcttcaccaagcgttacgttgttgacatcaagaacgtcaagacccttgatggtaaggacgttccagcatacactaccaccatcgacaccactgattctgttcgcccatccgtcctctccttctcctacgctgacaacggcctgaccctcaaggttaagttctctgagcctctggcatccgttggcaccgtcaagctttacgacggtaccactgaaatctccgtgtccccaaagttcaccgctggcgatgacgagatgaccatcaacctcgcatcttcctccgttccagttaacaaggacctgaccctcaagatcttcggcgctgtcgattacaacggtaacgttatcaaccctaacccagcagagctgactgttaagaagaccaccgtcgacatcaccaagccagttgttcagtccatcgaagctgtcaacactaagaccgtgaaggttaccttctctgagaagcttctctccgcacctaccatcaagatcggcggccagactgcttccgtttccgtcgactctactggtctggtttacaccgcaaccctcgcttccgcactgtccaagggcgtttacgctgtcgaggtttccgattacaaggaccttgcaggcaactctggtgacgcttacactaaggttgtccagctcaaggcagataacaccgctccaaagttcgtgtcctcccaggttgttaagatcaacggcgtcgaacacctggttctcaccttcgacgaggaggttaccactggctccaacatcaccgtcgttcagtcttccgacaagtacatcgatgaaaacaacgttctgaaggcagtcggtgctgaccttaagaccacctccgacaacttcaagctctacctgccaactgatggcaagtccaagtctgtggcactcaacatctcctccctgcctaagggcacctacaccgttacccttccaaacggtctcgtttccgacctggctgacaacccatacgcagagcgtaagcagatcactttcgtccgcggctctgattccctcaccaccaagcctgctctggacaaggactacgattccaacggcgttaaggcagacaacaacaacgagcttgttttcgctttcacccagaacctcgacgcatccgctctgaacctctctaacttcaacatcaacggtctgactgtcaccaaggcagttttcgatggcgacaccaagcacatccgtgttacccttgctccaggcgcaaacacttggactggtacccacgtcatcaccatctccaacatcaagaacacttccggcctcgtgatggacaccgttaccgttaacgaatacatgaaggagaacgtcgctccaaccttcactgcaaccctgacctccgctgatgttatccgcgttgacttctctgagcctgtcgcaaacgctaccatctcccgtgcactctccgctaacaacttcatcgttaaggttgacggcaacgtcgtgactgtttccaacgtttacgaagataacaacgcaaccaacctggtccagggttctaagggctacaagaccgtttaccttaagctccagtccccagttaccgacctgtccaagccaatcactctctccgctaccgacatcgtcgatgttaaccagaccggcgcaatcaccgacaacaacgttgtcggtaacaacgtgtctgctactgttgttaacgtcgcaaagaccggcgaatcaatcgaacaacaactgacagaactgcgaacgacgcttcgccatcatgaatatctttatcatgtgatggatgcgccggaaattcccgacgctgaatacgacaggctgatgcgcgaactgcgcgagctggaaaccaaacatccagaactgattacgcctgattcgcctactcaacgtgtaggcgctgcgccgctggcggctttcagccagatacgccatgaagtaccaatgctgtcactggataacgtttttgatgaagaaagctttcttgctttcaacaaacgtgtgcaggaccgtctgaaaaacaacgagaaagtcacctggtgctgtgagctgaagctggatggtcttgccgtcagtattctgtatgaaaatggcgttttagtcagtgccgcgacccgtggcgatggcaccaccggggaagatatcacgtctaatgtgcgtactattcgcgccattccgctgaagctgcacggagagaatatcccggcgcgtctggaagtgcgtggtgaagtgttcctgccgcaggcggggttcgaaaagattaacgaagatgcgcgacgcacgggcgggaaagtgtttgctaacccacgtaatgcggcagctggttcactgcgtcagcttgatccgcgtattacagcgaagcgaccgctcacttttttctgctatggcgttggtgttctggaaggtggcgagctgccggatactcatcttggccgtttactgcaatttaaaaagtgggggttgccggtcagcgatcgggtaacgctttgtgaatcggcggaagaagtgctggcgttctatcacaaagtggaagaagaccgcccgacgctgggctttgatatcgacggcgtggtgattaaggtcaactcactggcacagcaggagcagcttggctttgtcgcgcgtgccccgcgctgggcggtagcgtttaaattcccggcgcaggagcagatgacctttgtgcgtgacgtcgagtttcaggttgggcgtactggcgcgattacgcctgttgcgcgtctggaacctgtccatgttgcaggcgtgctggtgagtaacgcaaccttacacaatgcggatgaaatcgaacgtcttggtttacgcattggcgataaagtggtgattcgccgcgctggcgacgtgatcccgcaggtggttaacgtcgtgctttctgaacgcccggaagatacccgtgaggttgtattcccgacgcattgtccggtatgtggttctgacgttgagcgtgtggaaggtgaagcggttgcccgctgtaccggcggcctgatttgcggtgcgcagcgtaaagagtcgctgaaacactttgtttcccgccgtgcgatggatgttgacggaatgggcgacaaaatcatcgatcagctggttgaaaaagaatatgtccatactccggcagatctgttcaaactcaccgcaggcaaactgaccggactggagcgtatggggccaaaatcggcacaaaacgtggttaacgcgctggaaaaagcgaaagaaaccacctttgctcgcttcctctatgcacttggcatccgtgaagtcggcgaggccaccgcagcaggtctggcggcatatttcggcacgctggaagcgctggaagccgcttcgattgaagagctgcaaaaggtgcctgatgttggcattgtcgttgcatcccacgttcacaacttctttgccgaagaaagcaaccgcaatgtcatcagcgagctgttggcggaaggtgttcactggcctgcgccgatcgttatcaacgcggaagagattgacagcccgtttgctggtaaaaccgtggtgcttacgggcagcttaagccagatgtcgcgtgatgacgctaaagctcgactggtcgaactgggcgcgaaagtcgcgggcagcgtgtcgaagaaaaccgatctggtgatagcgggtgaagcggcaggatctaaactggcgaaggcgcaggaactgggcattgaagtcatcgacgaagcggaaatgctgcgtttgctgggtagccatcatcatcatcatcat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z