##gff-version 3
##sequence-region BBa_K525010 1 5226
BBa_K525010	.	CDS	3199	5208	.	+	0	ID=annotation2160525;Name=LigA domain
BBa_K525010	.	non_covalent_binding_site	76	81	.	+	0	ID=annotation2160529;Name=NgoMIV
BBa_K525010	.	tag	5209	5226	.	+	0	ID=annotation2160526;Name=His-Tag
BBa_K525010	.	ribosome_entry_site	53	64	.	+	0	ID=annotation2160521;Name=B0034
BBa_K525010	.	start_codon	73	75	.	+	0	ID=annotation2160522;Name=ATG
BBa_K525010	.	engineered_region	1	3193	.	+	0	ID=annotation2160527;Name=K525403
BBa_K525010	.	promoter	1	20	.	+	0	ID=annotation2160519;Name=T7 Promotor
BBa_K525010	.	engineered_region	3189	5226	.	+	0	ID=annotation2160528;Name=K525740
BBa_K525010	.	operator	20	44	.	+	0	ID=annotation2160520;Name=lac operator
BBa_K525010	.	sequence_feature	3193	3199	.	+	0	ID=annotation2160523;Name=Freiburg scar
BBa_K525010	.	CDS	73	3202	.	+	0	ID=annotation2160524;Name=SbpA domain
>BBa_K525010
taatacgactcactataggggaattgtgagcggataacaattcctactagagaaagaggagaaatactagagatggccg
gcgctcaggttaacgactacaacaagatctccggctacgcaaaggaggctgttcagtccctcgtcgaccagggtgttat
ccagggcgataccaacggcaacttcaacccactgaacaccgttactcgcgcacaggctgctgagatcttcaccaaggct
ctcgaactggaggcagacggtgacgtcaacttctccgatgttaagaagggcgcttggtactacaactctatcgcagctg
ttgtcgcaaacggcatcttcgagggtgtgtccgctaacgagttcgcaccaaacaagtcccttacccgttccgaggctgc
aaaggttctcgttgacgctttcggcctggagggctctgaatccctctcccagttcgcagacgcttcccaggtcaagggt
tgggcaaagtctgctctggagaccgcagttgctaacggcatcttcactggctccgaggaaaacggtaagcttaacctca
agcctaacgcagctatcacccgccaggatttcgcagttgtcttcgctcgtaccctggacctcgcagttgagaccgttga
cgcttccgtcaaggcaatcaacaacactaccgtggaggttaccttcgatgaagaggttgacaacgtcgaggctctgaag
ttcaagatcgaaggccttgaggttaagaacgcagctgttaagcagaccaacaagaaggtcgttgttctcactaccgagg
cacagaccgctgacaaggaatacgtcgtgaccctggatggcgaggagatcggtaagttcaagggcatcgaagcagttgt
tccaaagtccatcgtcctcaagactaccaacacccagggcaaggttggtaaccaggttaccctgactgctgacgtcggc
gttaaggcagctggcatcccagttaccttcaacgtcgacgcacctaccggctctcttaacaaggatgctgtggttgagg
tttacaccaacgcagagggcatcgcttcctactcctacactcagtacgcaccagacgctgacgatgtcaccgtttaccc
aaccggcgcacctcagctccgcgctttcggtccagtttactggggcgtcgacaacatcctgaccatcgaagaggttact
accggcaacaccctcgcaaacggtgttaagaagacctacaaggtcactttcaaggacccaaagaccggcgctgcactga
ccaacaagaagcttaacgtgtccttcgttgagaacaccaacgttgctttcaacgcaatctctaaggctactgtcaccaa
cccttcctccggcctcaccgttaccccataccagactaccaccggcctccaggaagagatccaggttaccactgattcc
aacggccaggcaaccttcgtcgtttctggcaccaacaccgctgttactccatacgtcttcgtggacggttcctcctccg
ttctcggcgtttctaccgtcaccggcaccaacaacgttactcaggcaaccaccgctaacaagaagtgggaggcaaccga
actgactgctaccgcagctcaggttaagttcgagggtgcacagcttaaccaccagatcaccgtcgagcgtgacggcgaa
gaggaggcagcagctcagtacggctccaagctcaacggtcgcgaatacaaggttaaggttctggataaggacggcaagc
cttacgcaaacggcctcgtcaacatcggtctggacgaggtgcttgatcgtaacctcaacaccaactccaaggctcagtt
cgcaaacgttactgacggcaccgctctgaccctcaacccaggcacctcccagcagggtaagatcaagctgaactctaag
ggcgaggcaactgttgtcctttacggcgctaagggtgaagttggcaccccagttgtctggatcgaccagaacacctccc
agaacaaccagtccggcgttctcgaggatggtgagcctttcttcaaggcaccagtttccaacttccagtacgaacgcgt
catgggcgctgagttcaccgtggagggcaagacttctaaccagaacgttggtaccgacggcatcgcaaactacgctttc
gcactgaccaaccagtccggcaagaccctcactaacctgatcggtaagatgacctacgaaatccgtaacacctccggcc
agccaatcgaggttaagacccctatctcctaccgcgaccagaacaacgtctcttccactcttttcaagatcgaggaata
cggctccatcaccatctccgctgatgttatctctacctccaacgcagctttcaccatcgagactgcaccaaacggtaag
gctgcaaccgttgtcgttaccccatccttcgttaccgctaacaacttcgacgcatccgctgacaacaacctcaacggcg
tcgtgactgatgcacgtgacttcaacaagcacgttaccgctcctgcactgaccgctaccttcggcaacaacaaggaggt
tggtactaactacaccggcaccgtccagtacttcaacaaggacaagaagaccatcactttcaacggcaagaacgcagtt
aagtacgctggtgaatctggcaagacctacaagtacttcggcctcggttccaccccaatctccaccgcagatgacttca
tcactcagctccagaacaacgctgcaaacggctccgttaccgtcacctacaaggttgttgacaacgaggtccagttcta
catcgtgtctttcaacaacgatggcgctgcaaacgctgttaagccagagcctaccgcagttgctccaactgacggtacc
gtcgcattcaccaacacctccttcaacgcttccgcaaaccagactatcaccgttaccgacgctgatcttaacgcagacg
ctaccgttgcagaaactaccaccgtcaaggttacctccggcactgttgtctacaccctcaccgctaccgagactggcgt
gaacaccggcgttttcaccgcaaccctgactgctgcacagctcgctgttctgtctgacggcgtcatcaccgcaacctac
gaggatgttaagaacaccactggctcctccgttacccgcaccgctaccgcaactcttaacaccgtcgttggtcaggtta
actacaacgcagcagctaccaccgcatactccaccggcgaatcaatcgaacaacaactgacagaactgcgaacgacgct
tcgccatcatgaatatctttatcatgtgatggatgcgccggaaattcccgacgctgaatacgacaggctgatgcgcgaa
ctgcgcgagctggaaaccaaacatccagaactgattacgcctgattcgcctactcaacgtgtaggcgctgcgccgctgg
cggctttcagccagatacgccatgaagtaccaatgctgtcactggataacgtttttgatgaagaaagctttcttgcttt
caacaaacgtgtgcaggaccgtctgaaaaacaacgagaaagtcacctggtgctgtgagctgaagctggatggtcttgcc
gtcagtattctgtatgaaaatggcgttttagtcagtgccgcgacccgtggcgatggcaccaccggggaagatatcacgt
ctaatgtgcgtactattcgcgccattccgctgaagctgcacggagagaatatcccggcgcgtctggaagtgcgtggtga
agtgttcctgccgcaggcggggttcgaaaagattaacgaagatgcgcgacgcacgggcgggaaagtgtttgctaaccca
cgtaatgcggcagctggttcactgcgtcagcttgatccgcgtattacagcgaagcgaccgctcacttttttctgctatg
gcgttggtgttctggaaggtggcgagctgccggatactcatcttggccgtttactgcaatttaaaaagtgggggttgcc
ggtcagcgatcgggtaacgctttgtgaatcggcggaagaagtgctggcgttctatcacaaagtggaagaagaccgcccg
acgctgggctttgatatcgacggcgtggtgattaaggtcaactcactggcacagcaggagcagcttggctttgtcgcgc
gtgccccgcgctgggcggtagcgtttaaattcccggcgcaggagcagatgacctttgtgcgtgacgtcgagtttcaggt
tgggcgtactggcgcgattacgcctgttgcgcgtctggaacctgtccatgttgcaggcgtgctggtgagtaacgcaacc
ttacacaatgcggatgaaatcgaacgtcttggtttacgcattggcgataaagtggtgattcgccgcgctggcgacgtga
tcccgcaggtggttaacgtcgtgctttctgaacgcccggaagatacccgtgaggttgtattcccgacgcattgtccggt
atgtggttctgacgttgagcgtgtggaaggtgaagcggttgcccgctgtaccggcggcctgatttgcggtgcgcagcgt
aaagagtcgctgaaacactttgtttcccgccgtgcgatggatgttgacggaatgggcgacaaaatcatcgatcagctgg
ttgaaaaagaatatgtccatactccggcagatctgttcaaactcaccgcaggcaaactgaccggactggagcgtatggg
gccaaaatcggcacaaaacgtggttaacgcgctggaaaaagcgaaagaaaccacctttgctcgcttcctctatgcactt
ggcatccgtgaagtcggcgaggccaccgcagcaggtctggcggcatatttcggcacgctggaagcgctggaagccgctt
cgattgaagagctgcaaaaggtgcctgatgttggcattgtcgttgcatcccacgttcacaacttctttgccgaagaaag
caaccgcaatgtcatcagcgagctgttggcggaaggtgttcactggcctgcgccgatcgttatcaacgcggaagagatt
gacagcccgtttgctggtaaaaccgtggtgcttacgggcagcttaagccagatgtcgcgtgatgacgctaaagctcgac
tggtcgaactgggcgcgaaagtcgcgggcagcgtgtcgaagaaaaccgatctggtgatagcgggtgaagcggcaggatc
taaactggcgaaggcgcaggaactgggcattgaagtcatcgacgaagcggaaatgctgcgtttgctgggtagccatcat
catcatcatcat