BBa_K525010 1 BBa_K525010 Fusion Protein of S-Layer SbpA and LigA with His-Tag 2011-10-24T11:00:00Z 2015-05-08T01:12:34Z SbpA was synthesized, LigA is the native ligase of E. coli. Fusion Protein of S-Layer SbpA and LigA with His-Tag false false _690_ 0 8718 9 Not in stock false Assembled with Freiburg Standard Assembly (RFC 25) false Manuel Wittchen annotation2160520 1 lac operator range2160520 1 20 44 annotation2160523 1 Freiburg scar range2160523 1 3193 3199 annotation2160526 1 His-Tag range2160526 1 5209 5226 annotation2160519 1 T7 Promotor range2160519 1 1 20 annotation2160529 1 NgoMIV range2160529 1 76 81 annotation2160528 1 K525740 range2160528 1 3189 5226 annotation2160525 1 LigA domain range2160525 1 3199 5208 annotation2160522 1 ATG range2160522 1 73 75 annotation2160524 1 SbpA domain range2160524 1 73 3202 annotation2160527 1 K525403 range2160527 1 1 3193 annotation2160521 1 B0034 range2160521 1 53 64 BBa_K525010_sequence 1 taatacgactcactataggggaattgtgagcggataacaattcctactagagaaagaggagaaatactagagatggccggcgctcaggttaacgactacaacaagatctccggctacgcaaaggaggctgttcagtccctcgtcgaccagggtgttatccagggcgataccaacggcaacttcaacccactgaacaccgttactcgcgcacaggctgctgagatcttcaccaaggctctcgaactggaggcagacggtgacgtcaacttctccgatgttaagaagggcgcttggtactacaactctatcgcagctgttgtcgcaaacggcatcttcgagggtgtgtccgctaacgagttcgcaccaaacaagtcccttacccgttccgaggctgcaaaggttctcgttgacgctttcggcctggagggctctgaatccctctcccagttcgcagacgcttcccaggtcaagggttgggcaaagtctgctctggagaccgcagttgctaacggcatcttcactggctccgaggaaaacggtaagcttaacctcaagcctaacgcagctatcacccgccaggatttcgcagttgtcttcgctcgtaccctggacctcgcagttgagaccgttgacgcttccgtcaaggcaatcaacaacactaccgtggaggttaccttcgatgaagaggttgacaacgtcgaggctctgaagttcaagatcgaaggccttgaggttaagaacgcagctgttaagcagaccaacaagaaggtcgttgttctcactaccgaggcacagaccgctgacaaggaatacgtcgtgaccctggatggcgaggagatcggtaagttcaagggcatcgaagcagttgttccaaagtccatcgtcctcaagactaccaacacccagggcaaggttggtaaccaggttaccctgactgctgacgtcggcgttaaggcagctggcatcccagttaccttcaacgtcgacgcacctaccggctctcttaacaaggatgctgtggttgaggtttacaccaacgcagagggcatcgcttcctactcctacactcagtacgcaccagacgctgacgatgtcaccgtttacccaaccggcgcacctcagctccgcgctttcggtccagtttactggggcgtcgacaacatcctgaccatcgaagaggttactaccggcaacaccctcgcaaacggtgttaagaagacctacaaggtcactttcaaggacccaaagaccggcgctgcactgaccaacaagaagcttaacgtgtccttcgttgagaacaccaacgttgctttcaacgcaatctctaaggctactgtcaccaacccttcctccggcctcaccgttaccccataccagactaccaccggcctccaggaagagatccaggttaccactgattccaacggccaggcaaccttcgtcgtttctggcaccaacaccgctgttactccatacgtcttcgtggacggttcctcctccgttctcggcgtttctaccgtcaccggcaccaacaacgttactcaggcaaccaccgctaacaagaagtgggaggcaaccgaactgactgctaccgcagctcaggttaagttcgagggtgcacagcttaaccaccagatcaccgtcgagcgtgacggcgaagaggaggcagcagctcagtacggctccaagctcaacggtcgcgaatacaaggttaaggttctggataaggacggcaagccttacgcaaacggcctcgtcaacatcggtctggacgaggtgcttgatcgtaacctcaacaccaactccaaggctcagttcgcaaacgttactgacggcaccgctctgaccctcaacccaggcacctcccagcagggtaagatcaagctgaactctaagggcgaggcaactgttgtcctttacggcgctaagggtgaagttggcaccccagttgtctggatcgaccagaacacctcccagaacaaccagtccggcgttctcgaggatggtgagcctttcttcaaggcaccagtttccaacttccagtacgaacgcgtcatgggcgctgagttcaccgtggagggcaagacttctaaccagaacgttggtaccgacggcatcgcaaactacgctttcgcactgaccaaccagtccggcaagaccctcactaacctgatcggtaagatgacctacgaaatccgtaacacctccggccagccaatcgaggttaagacccctatctcctaccgcgaccagaacaacgtctcttccactcttttcaagatcgaggaatacggctccatcaccatctccgctgatgttatctctacctccaacgcagctttcaccatcgagactgcaccaaacggtaaggctgcaaccgttgtcgttaccccatccttcgttaccgctaacaacttcgacgcatccgctgacaacaacctcaacggcgtcgtgactgatgcacgtgacttcaacaagcacgttaccgctcctgcactgaccgctaccttcggcaacaacaaggaggttggtactaactacaccggcaccgtccagtacttcaacaaggacaagaagaccatcactttcaacggcaagaacgcagttaagtacgctggtgaatctggcaagacctacaagtacttcggcctcggttccaccccaatctccaccgcagatgacttcatcactcagctccagaacaacgctgcaaacggctccgttaccgtcacctacaaggttgttgacaacgaggtccagttctacatcgtgtctttcaacaacgatggcgctgcaaacgctgttaagccagagcctaccgcagttgctccaactgacggtaccgtcgcattcaccaacacctccttcaacgcttccgcaaaccagactatcaccgttaccgacgctgatcttaacgcagacgctaccgttgcagaaactaccaccgtcaaggttacctccggcactgttgtctacaccctcaccgctaccgagactggcgtgaacaccggcgttttcaccgcaaccctgactgctgcacagctcgctgttctgtctgacggcgtcatcaccgcaacctacgaggatgttaagaacaccactggctcctccgttacccgcaccgctaccgcaactcttaacaccgtcgttggtcaggttaactacaacgcagcagctaccaccgcatactccaccggcgaatcaatcgaacaacaactgacagaactgcgaacgacgcttcgccatcatgaatatctttatcatgtgatggatgcgccggaaattcccgacgctgaatacgacaggctgatgcgcgaactgcgcgagctggaaaccaaacatccagaactgattacgcctgattcgcctactcaacgtgtaggcgctgcgccgctggcggctttcagccagatacgccatgaagtaccaatgctgtcactggataacgtttttgatgaagaaagctttcttgctttcaacaaacgtgtgcaggaccgtctgaaaaacaacgagaaagtcacctggtgctgtgagctgaagctggatggtcttgccgtcagtattctgtatgaaaatggcgttttagtcagtgccgcgacccgtggcgatggcaccaccggggaagatatcacgtctaatgtgcgtactattcgcgccattccgctgaagctgcacggagagaatatcccggcgcgtctggaagtgcgtggtgaagtgttcctgccgcaggcggggttcgaaaagattaacgaagatgcgcgacgcacgggcgggaaagtgtttgctaacccacgtaatgcggcagctggttcactgcgtcagcttgatccgcgtattacagcgaagcgaccgctcacttttttctgctatggcgttggtgttctggaaggtggcgagctgccggatactcatcttggccgtttactgcaatttaaaaagtgggggttgccggtcagcgatcgggtaacgctttgtgaatcggcggaagaagtgctggcgttctatcacaaagtggaagaagaccgcccgacgctgggctttgatatcgacggcgtggtgattaaggtcaactcactggcacagcaggagcagcttggctttgtcgcgcgtgccccgcgctgggcggtagcgtttaaattcccggcgcaggagcagatgacctttgtgcgtgacgtcgagtttcaggttgggcgtactggcgcgattacgcctgttgcgcgtctggaacctgtccatgttgcaggcgtgctggtgagtaacgcaaccttacacaatgcggatgaaatcgaacgtcttggtttacgcattggcgataaagtggtgattcgccgcgctggcgacgtgatcccgcaggtggttaacgtcgtgctttctgaacgcccggaagatacccgtgaggttgtattcccgacgcattgtccggtatgtggttctgacgttgagcgtgtggaaggtgaagcggttgcccgctgtaccggcggcctgatttgcggtgcgcagcgtaaagagtcgctgaaacactttgtttcccgccgtgcgatggatgttgacggaatgggcgacaaaatcatcgatcagctggttgaaaaagaatatgtccatactccggcagatctgttcaaactcaccgcaggcaaactgaccggactggagcgtatggggccaaaatcggcacaaaacgtggttaacgcgctggaaaaagcgaaagaaaccacctttgctcgcttcctctatgcacttggcatccgtgaagtcggcgaggccaccgcagcaggtctggcggcatatttcggcacgctggaagcgctggaagccgcttcgattgaagagctgcaaaaggtgcctgatgttggcattgtcgttgcatcccacgttcacaacttctttgccgaagaaagcaaccgcaatgtcatcagcgagctgttggcggaaggtgttcactggcctgcgccgatcgttatcaacgcggaagagattgacagcccgtttgctggtaaaaccgtggtgcttacgggcagcttaagccagatgtcgcgtgatgacgctaaagctcgactggtcgaactgggcgcgaaagtcgcgggcagcgtgtcgaagaaaaccgatctggtgatagcgggtgaagcggcaggatctaaactggcgaaggcgcaggaactgggcattgaagtcatcgacgaagcggaaatgctgcgtttgctgggtagccatcatcatcatcatcat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z