LOCUS       BBa_K525234             2189 bp    DNA     linear       22-Nov-2024
DEFINITION  Fusion Protein of mRFP, S-layer cspB from Corynebacterium 
            halotolerans with TAT-sequence, PT7, RBS
ACCESSION   BBa_K525234
VERSION     BBa_K525234.1  
FEATURES             Location/Qualifiers
     misc_feature    809..2189
                     /label=K525222
     promoter        1..20
                     /label=T7 promoter
     misc_feature    21..33
                     /label=BBa_B0034
     RBS             25..28
                     /label=RBS
     misc_feature    34..128
                     /label=TAT-sequence
     CDS             129..799
                     /label=mRFP
     misc_feature    129..799
                     /label=BBa_E1010
     misc_feature    800..808
                     /label=Linker
     CDS             809..2189
                     /label=cspB domain
     misc_feature    2187..2189
                     /label=TAA
ORIGIN
        1 taatacgact cactataggg aaagaggaga aaatgatcaa gaaccgcatc cgcaccgcgg
       61 ctctcgcagg tgccatcgct gtcgccaccg gtgtctccgg cctggccgtc ccggccatgg
      121 cgcaggacgc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
      181 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
      241 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
      301 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
      361 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
      421 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
      481 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
      541 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
      601 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
      661 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
      721 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
      781 gtcgtcactc caccggtgct gcaggtgcac aggacgccca ggttgccact gcacagttca
      841 ccgtcgagga tggctccgcc gccgtctccg ccgacccgca gaacatcgac gagaacgagc
      901 tgcgtgagct gaccgacgcc actggtgact acctccgtgg ctgggatcgt gccgacggtg
      961 tccgcgacat cgtccgcgcc taccttgaga ccggtgaggc cggcttcgac ccgtccgagg
     1021 aagctgctca ggccgctttc gagggcgccc gcgctgacgc tgccgctcag ctcgccacct
     1081 cggcagagaa catcaccaag gcccgtcagt ctgtcgcgtt cgctctgcag gccgaccaca
     1141 ctgccaccaa cgcatggaac gccctgcgtg gtgccctgga tgacacccga gatgtggtga
     1201 acccgctgat cgaggccacc aacgaggcca accggtacca gggcgaggag ttctgggact
     1261 ttgaggccct gtacaccacc ggtggtatcg gccactccga gtctgacctg gctgccgcct
     1321 accgcgatct gctcgagctg cgggacgaca tcaacaccca gtacgagacc gctggtgagt
     1381 ggcagattga cgaccgctac aaccgcgtca ctcgcgagca catggccgcc ctcgacgttc
     1441 tgaaggagga ggttgatgct caggtggccg ccatcgaggg tgtctaccag gaggccatcg
     1501 acaccaaccg tgaggcccag cgttccgacg tcctggttcg ccagctctac ctggagcgcg
     1561 ccactgctca gcgcgacacc ctccgcgttg tcgaagccac cttccgcacc gccgcccgcc
     1621 aggtcgagct gtacggtgat aatgacacgc tggtgggtaa cgacgagcag tccctgcggg
     1681 gtctctacac cgctctgctc gctccgaatg gcccgctgct caacggcctg agcatgaacc
     1741 tcgatttcct cgatgaggct gatcgtgaga cctacttcgg ccacgacgcc tggtggaccg
     1801 acctgggcaa caacgctgac ggtcgcttcc agatttaccg cgagcacctg accttcgcgt
     1861 ccgagagcta ctccaagatc ttcgcgaacg ccaccgtctg gcagaacgag ctctcccgtg
     1921 tcgagctgct cgatcagaag gctgccgacg aggtcgccga gcgtgaggca gagcgagagg
     1981 ctgagaaggc cgccgccgag gaggctgcgc gtcaggccaa ggaggccgct gagcgtgagg
     2041 agcggatcgc caaggccctt gaggagctgg ccaaggcccg cgaggaggcc ggcaaggaca
     2101 acggcaacaa caacggtgac gacaacgacg acgacgacgt caacgagggt tcctctgagg
     2161 gctcctccgt cggtggcctg ggcatctaa
//