LOCUS       BBa_K525305             3129 bp    DNA     linear       27-Apr-2024
DEFINITION  Fusion Protein of S-Layer SgsE and mCitrine
ACCESSION   BBa_K525305
VERSION     BBa_K525305.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=T7 promoter
     misc_feature    20..44
                     /label=lac operator
     RBS             56..59
                     /label=RBS
     misc_feature    53..64
                     /label=B0034
     misc_feature    76..81
                     /label=NgoMIV
     misc_feature    73..75
                     /label=ATG
     CDS             73..2400
                     /label=SgsE domain
     misc_feature    2401..2406
                     /label=Freiburg scar
     CDS             2407..3129
                     /label=mCitrine (YFP)
     misc_feature    3127..3129
                     /label=TAA
     misc_feature    3121..3126
                     /label=AgeI
     misc_feature    73..2400
                     /label=K525301
     misc_feature    2407..3129
                     /label=J18931
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcctactag agaaagagga
       61 gaaatactag agatggccgg cgctaccaag ctcgacaaga tgcgccagga gctgaaggca
      121 gctgttgacg caaaggatct caagaaggct gaggaactgt accacaagat ctcctacgag
      181 cttaagaccc gtactgttat cctcgaccgc gtctacggcc agtccacccg tgagctgctc
      241 cgctccacct tcaaggcaga cgctcaggca ctgcgtgatt ctcttatcta cgacatcacc
      301 gttgctatga aggcacgcga agctcaggac gcagttaagg ctggtaacct cgataaggca
      361 aaggctgcac tggaccaggt caaccagtac gtttccaagg ttactgacgc tttcaaggca
      421 gagctccaga aggctgcaca ggatgctaag gcagcttacg aggcagctct gccaccaaag
      481 gtcgaatccg tgaccgcagt taacgctaag acccttgaga tcaagttcaa caaggcagtt
      541 gacgctgcaa ccgtcatcga caacaagggc acttccgata cctctgacga cgttgttaag
      601 gctaccgcaa tcaccctcaa ggctatcgat gaccaggtcc ctgtttccac tgttaaggca
      661 tccctgtccg acgataagaa gaccctcaag ctggtcgtgg acggcgctca gttcttcacc
      721 aagcgttacg ttgttgacat caagaacgtc aagacccttg atggtaagga cgttccagca
      781 tacactacca ccatcgacac cactgattct gttcgcccat ccgtcctctc cttctcctac
      841 gctgacaacg gcctgaccct caaggttaag ttctctgagc ctctggcatc cgttggcacc
      901 gtcaagcttt acgacggtac cactgaaatc tccgtgtccc caaagttcac cgctggcgat
      961 gacgagatga ccatcaacct cgcatcttcc tccgttccag ttaacaagga cctgaccctc
     1021 aagatcttcg gcgctgtcga ttacaacggt aacgttatca accctaaccc agcagagctg
     1081 actgttaaga agaccaccgt cgacatcacc aagccagttg ttcagtccat cgaagctgtc
     1141 aacactaaga ccgtgaaggt taccttctct gagaagcttc tctccgcacc taccatcaag
     1201 atcggcggcc agactgcttc cgtttccgtc gactctactg gtctggttta caccgcaacc
     1261 ctcgcttccg cactgtccaa gggcgtttac gctgtcgagg tttccgatta caaggacctt
     1321 gcaggcaact ctggtgacgc ttacactaag gttgtccagc tcaaggcaga taacaccgct
     1381 ccaaagttcg tgtcctccca ggttgttaag atcaacggcg tcgaacacct ggttctcacc
     1441 ttcgacgagg aggttaccac tggctccaac atcaccgtcg ttcagtcttc cgacaagtac
     1501 atcgatgaaa acaacgttct gaaggcagtc ggtgctgacc ttaagaccac ctccgacaac
     1561 ttcaagctct acctgccaac tgatggcaag tccaagtctg tggcactcaa catctcctcc
     1621 ctgcctaagg gcacctacac cgttaccctt ccaaacggtc tcgtttccga cctggctgac
     1681 aacccatacg cagagcgtaa gcagatcact ttcgtccgcg gctctgattc cctcaccacc
     1741 aagcctgctc tggacaagga ctacgattcc aacggcgtta aggcagacaa caacaacgag
     1801 cttgttttcg ctttcaccca gaacctcgac gcatccgctc tgaacctctc taacttcaac
     1861 atcaacggtc tgactgtcac caaggcagtt ttcgatggcg acaccaagca catccgtgtt
     1921 acccttgctc caggcgcaaa cacttggact ggtacccacg tcatcaccat ctccaacatc
     1981 aagaacactt ccggcctcgt gatggacacc gttaccgtta acgaatacat gaaggagaac
     2041 gtcgctccaa ccttcactgc aaccctgacc tccgctgatg ttatccgcgt tgacttctct
     2101 gagcctgtcg caaacgctac catctcccgt gcactctccg ctaacaactt catcgttaag
     2161 gttgacggca acgtcgtgac tgtttccaac gtttacgaag ataacaacgc aaccaacctg
     2221 gtccagggtt ctaagggcta caagaccgtt taccttaagc tccagtcccc agttaccgac
     2281 ctgtccaagc caatcactct ctccgctacc gacatcgtcg atgttaacca gaccggcgca
     2341 atcaccgaca acaacgttgt cggtaacaac gtgtctgcta ctgttgttaa cgtcgcaaag
     2401 accggcgtga gcaaaggcga agaactgttt accggcgtgg tgccgattct ggtggaactg
     2461 gatggtgatg tgaacggcca taaatttagc gtgagcggcg aaggcgaagg tgatgcgacc
     2521 tatggcaaac tgaccctgaa atttatttgc accacgggta aactgccggt tccgtggccg
     2581 accctggtga ccacctttgg ctatggcctg atgtgctttg cgcgttatcc ggatcatatg
     2641 aaacagcacg atttctttaa aagcgccatg ccggaaggct atgtgcagga acgcaccatc
     2701 ttttttaaag atgatggcaa ctataaaacc cgtgcggaag tgaaatttga aggcgatacc
     2761 ctggtgaacc gtattgaact gaaaggcatc gatttcaaag aagatggcaa cattctgggc
     2821 cataaactgg aatataacta caacagccat aacgtgtata tcatggcgga taaacagaaa
     2881 aacggcatca aagtgaactt taaaatccgc cacaacattg aagatggcag cgtgcagctg
     2941 gccgatcatt atcagcagaa caccccgatt ggtgatggcc cggtgctgct gccggataac
     3001 cattatctga gctaccagag caaactgagc aaagatccga acgaaaaacg tgatcacatg
     3061 gtgctgctgg aatttgtgac cgcagccggt attaccctgg gcatggatga actgtacaaa
     3121 accggttaa
//