##gff-version 3
##sequence-region BBa_K525305 1 3129
BBa_K525305	.	engineered_region	73	2400	.	+	0	ID=annotation2127339;Name=K525301
BBa_K525305	.	CDS	2407	3129	.	+	0	ID=annotation2127336;Name=mCitrine (YFP)
BBa_K525305	.	operator	20	44	.	+	0	ID=annotation2127328;Name=lac operator
BBa_K525305	.	engineered_region	53	64	.	+	0	ID=annotation2127331;Name=B0034
BBa_K525305	.	promoter	1	20	.	+	0	ID=annotation2127327;Name=T7 promoter
BBa_K525305	.	CDS	73	2400	.	+	0	ID=annotation2127334;Name=SgsE domain
BBa_K525305	.	start_codon	73	75	.	+	0	ID=annotation2127333;Name=ATG
BBa_K525305	.	stop_codon	3127	3129	.	+	0	ID=annotation2127337;Name=TAA
BBa_K525305	.	sequence_feature	2401	2406	.	+	0	ID=annotation2127335;Name=Freiburg scar
BBa_K525305	.	non_covalent_binding_site	76	81	.	+	0	ID=annotation2127332;Name=NgoMIV
BBa_K525305	.	non_covalent_binding_site	3121	3126	.	+	0	ID=annotation2127338;Name=AgeI
BBa_K525305	.	engineered_region	2407	3129	.	+	0	ID=annotation2127341;Name=J18931
BBa_K525305	.	ribosome_entry_site	56	59	.	+	0	ID=annotation2127330;Name=RBS
>BBa_K525305
taatacgactcactataggggaattgtgagcggataacaattcctactagagaaagaggagaaatactagagatggccg
gcgctaccaagctcgacaagatgcgccaggagctgaaggcagctgttgacgcaaaggatctcaagaaggctgaggaact
gtaccacaagatctcctacgagcttaagacccgtactgttatcctcgaccgcgtctacggccagtccacccgtgagctg
ctccgctccaccttcaaggcagacgctcaggcactgcgtgattctcttatctacgacatcaccgttgctatgaaggcac
gcgaagctcaggacgcagttaaggctggtaacctcgataaggcaaaggctgcactggaccaggtcaaccagtacgtttc
caaggttactgacgctttcaaggcagagctccagaaggctgcacaggatgctaaggcagcttacgaggcagctctgcca
ccaaaggtcgaatccgtgaccgcagttaacgctaagacccttgagatcaagttcaacaaggcagttgacgctgcaaccg
tcatcgacaacaagggcacttccgatacctctgacgacgttgttaaggctaccgcaatcaccctcaaggctatcgatga
ccaggtccctgtttccactgttaaggcatccctgtccgacgataagaagaccctcaagctggtcgtggacggcgctcag
ttcttcaccaagcgttacgttgttgacatcaagaacgtcaagacccttgatggtaaggacgttccagcatacactacca
ccatcgacaccactgattctgttcgcccatccgtcctctccttctcctacgctgacaacggcctgaccctcaaggttaa
gttctctgagcctctggcatccgttggcaccgtcaagctttacgacggtaccactgaaatctccgtgtccccaaagttc
accgctggcgatgacgagatgaccatcaacctcgcatcttcctccgttccagttaacaaggacctgaccctcaagatct
tcggcgctgtcgattacaacggtaacgttatcaaccctaacccagcagagctgactgttaagaagaccaccgtcgacat
caccaagccagttgttcagtccatcgaagctgtcaacactaagaccgtgaaggttaccttctctgagaagcttctctcc
gcacctaccatcaagatcggcggccagactgcttccgtttccgtcgactctactggtctggtttacaccgcaaccctcg
cttccgcactgtccaagggcgtttacgctgtcgaggtttccgattacaaggaccttgcaggcaactctggtgacgctta
cactaaggttgtccagctcaaggcagataacaccgctccaaagttcgtgtcctcccaggttgttaagatcaacggcgtc
gaacacctggttctcaccttcgacgaggaggttaccactggctccaacatcaccgtcgttcagtcttccgacaagtaca
tcgatgaaaacaacgttctgaaggcagtcggtgctgaccttaagaccacctccgacaacttcaagctctacctgccaac
tgatggcaagtccaagtctgtggcactcaacatctcctccctgcctaagggcacctacaccgttacccttccaaacggt
ctcgtttccgacctggctgacaacccatacgcagagcgtaagcagatcactttcgtccgcggctctgattccctcacca
ccaagcctgctctggacaaggactacgattccaacggcgttaaggcagacaacaacaacgagcttgttttcgctttcac
ccagaacctcgacgcatccgctctgaacctctctaacttcaacatcaacggtctgactgtcaccaaggcagttttcgat
ggcgacaccaagcacatccgtgttacccttgctccaggcgcaaacacttggactggtacccacgtcatcaccatctcca
acatcaagaacacttccggcctcgtgatggacaccgttaccgttaacgaatacatgaaggagaacgtcgctccaacctt
cactgcaaccctgacctccgctgatgttatccgcgttgacttctctgagcctgtcgcaaacgctaccatctcccgtgca
ctctccgctaacaacttcatcgttaaggttgacggcaacgtcgtgactgtttccaacgtttacgaagataacaacgcaa
ccaacctggtccagggttctaagggctacaagaccgtttaccttaagctccagtccccagttaccgacctgtccaagcc
aatcactctctccgctaccgacatcgtcgatgttaaccagaccggcgcaatcaccgacaacaacgttgtcggtaacaac
gtgtctgctactgttgttaacgtcgcaaagaccggcgtgagcaaaggcgaagaactgtttaccggcgtggtgccgattc
tggtggaactggatggtgatgtgaacggccataaatttagcgtgagcggcgaaggcgaaggtgatgcgacctatggcaa
actgaccctgaaatttatttgcaccacgggtaaactgccggttccgtggccgaccctggtgaccacctttggctatggc
ctgatgtgctttgcgcgttatccggatcatatgaaacagcacgatttctttaaaagcgccatgccggaaggctatgtgc
aggaacgcaccatcttttttaaagatgatggcaactataaaacccgtgcggaagtgaaatttgaaggcgataccctggt
gaaccgtattgaactgaaaggcatcgatttcaaagaagatggcaacattctgggccataaactggaatataactacaac
agccataacgtgtatatcatggcggataaacagaaaaacggcatcaaagtgaactttaaaatccgccacaacattgaag
atggcagcgtgcagctggccgatcattatcagcagaacaccccgattggtgatggcccggtgctgctgccggataacca
ttatctgagctaccagagcaaactgagcaaagatccgaacgaaaaacgtgatcacatggtgctgctggaatttgtgacc
gcagccggtattaccctgggcatggatgaactgtacaaaaccggttaa