LOCUS BBa_K525311 4062 bp DNA linear 08-May-2024 DEFINITION Fusion Protein of S-Layer SgsE and Firefly-Luciferase ACCESSION BBa_K525311 VERSION BBa_K525311.1 FEATURES Location/Qualifiers promoter 1..20 /label=pT7 misc_feature 20..44 /label=lac opterator misc_feature 53..64 /label=B0034 RBS 56..59 /label=RBS CDS 73..2400 /label=SgsE domain misc_feature 73..2400 /label=K525301 misc_feature 2401..2406 /label=Freiburg scar misc_feature 73..75 /label=ATG misc_feature 76..81 /label=NgoMIV CDS 2407..4062 /label=Luciferase misc_feature 2407..4062 /label=K525999 misc_feature 4060..4062 /label=TAA misc_feature 4054..4059 /label=AgeI ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcctactag agaaagagga 61 gaaatactag agatggccgg cgctaccaag ctcgacaaga tgcgccagga gctgaaggca 121 gctgttgacg caaaggatct caagaaggct gaggaactgt accacaagat ctcctacgag 181 cttaagaccc gtactgttat cctcgaccgc gtctacggcc agtccacccg tgagctgctc 241 cgctccacct tcaaggcaga cgctcaggca ctgcgtgatt ctcttatcta cgacatcacc 301 gttgctatga aggcacgcga agctcaggac gcagttaagg ctggtaacct cgataaggca 361 aaggctgcac tggaccaggt caaccagtac gtttccaagg ttactgacgc tttcaaggca 421 gagctccaga aggctgcaca ggatgctaag gcagcttacg aggcagctct gccaccaaag 481 gtcgaatccg tgaccgcagt taacgctaag acccttgaga tcaagttcaa caaggcagtt 541 gacgctgcaa ccgtcatcga caacaagggc acttccgata cctctgacga cgttgttaag 601 gctaccgcaa tcaccctcaa ggctatcgat gaccaggtcc ctgtttccac tgttaaggca 661 tccctgtccg acgataagaa gaccctcaag ctggtcgtgg acggcgctca gttcttcacc 721 aagcgttacg ttgttgacat caagaacgtc aagacccttg atggtaagga cgttccagca 781 tacactacca ccatcgacac cactgattct gttcgcccat ccgtcctctc cttctcctac 841 gctgacaacg gcctgaccct caaggttaag ttctctgagc ctctggcatc cgttggcacc 901 gtcaagcttt acgacggtac cactgaaatc tccgtgtccc caaagttcac cgctggcgat 961 gacgagatga ccatcaacct cgcatcttcc tccgttccag ttaacaagga cctgaccctc 1021 aagatcttcg gcgctgtcga ttacaacggt aacgttatca accctaaccc agcagagctg 1081 actgttaaga agaccaccgt cgacatcacc aagccagttg ttcagtccat cgaagctgtc 1141 aacactaaga ccgtgaaggt taccttctct gagaagcttc tctccgcacc taccatcaag 1201 atcggcggcc agactgcttc cgtttccgtc gactctactg gtctggttta caccgcaacc 1261 ctcgcttccg cactgtccaa gggcgtttac gctgtcgagg tttccgatta caaggacctt 1321 gcaggcaact ctggtgacgc ttacactaag gttgtccagc tcaaggcaga taacaccgct 1381 ccaaagttcg tgtcctccca ggttgttaag atcaacggcg tcgaacacct ggttctcacc 1441 ttcgacgagg aggttaccac tggctccaac atcaccgtcg ttcagtcttc cgacaagtac 1501 atcgatgaaa acaacgttct gaaggcagtc ggtgctgacc ttaagaccac ctccgacaac 1561 ttcaagctct acctgccaac tgatggcaag tccaagtctg tggcactcaa catctcctcc 1621 ctgcctaagg gcacctacac cgttaccctt ccaaacggtc tcgtttccga cctggctgac 1681 aacccatacg cagagcgtaa gcagatcact ttcgtccgcg gctctgattc cctcaccacc 1741 aagcctgctc tggacaagga ctacgattcc aacggcgtta aggcagacaa caacaacgag 1801 cttgttttcg ctttcaccca gaacctcgac gcatccgctc tgaacctctc taacttcaac 1861 atcaacggtc tgactgtcac caaggcagtt ttcgatggcg acaccaagca catccgtgtt 1921 acccttgctc caggcgcaaa cacttggact ggtacccacg tcatcaccat ctccaacatc 1981 aagaacactt ccggcctcgt gatggacacc gttaccgtta acgaatacat gaaggagaac 2041 gtcgctccaa ccttcactgc aaccctgacc tccgctgatg ttatccgcgt tgacttctct 2101 gagcctgtcg caaacgctac catctcccgt gcactctccg ctaacaactt catcgttaag 2161 gttgacggca acgtcgtgac tgtttccaac gtttacgaag ataacaacgc aaccaacctg 2221 gtccagggtt ctaagggcta caagaccgtt taccttaagc tccagtcccc agttaccgac 2281 ctgtccaagc caatcactct ctccgctacc gacatcgtcg atgttaacca gaccggcgca 2341 atcaccgaca acaacgttgt cggtaacaac gtgtctgcta ctgttgttaa cgtcgcaaag 2401 accggcgaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat 2461 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca 2521 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg 2581 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc 2641 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga 2701 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc 2761 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg 2821 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag 2881 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac 2941 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct 3001 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc 3061 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt 3121 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga 3181 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat 3241 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact 3301 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc 3361 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga 3421 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg 3481 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg 3541 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc 3601 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat 3661 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag 3721 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc 3781 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa 3841 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg 3901 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg 3961 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc 4021 ataaaggcca agaagggcgg aaagatcgcc gtgaccggtt aa //