##gff-version 3
##sequence-region BBa_K525311 1 4062
BBa_K525311	.	engineered_region	73	2400	.	+	0	ID=annotation2137362;Name=K525301
BBa_K525311	.	CDS	2407	4062	.	+	0	ID=annotation2137366;Name=Luciferase
BBa_K525311	.	CDS	73	2400	.	+	0	ID=annotation2137354;Name=SgsE domain
BBa_K525311	.	promoter	1	20	.	+	0	ID=annotation2137343;Name=pT7
BBa_K525311	.	engineered_region	53	64	.	+	0	ID=annotation2137352;Name=B0034
BBa_K525311	.	engineered_region	2407	4062	.	+	0	ID=annotation2137367;Name=K525999
BBa_K525311	.	operator	20	44	.	+	0	ID=annotation2137351;Name=lac opterator
BBa_K525311	.	stop_codon	4060	4062	.	+	0	ID=annotation2137368;Name=TAA
BBa_K525311	.	start_codon	73	75	.	+	0	ID=annotation2137364;Name=ATG
BBa_K525311	.	sequence_feature	2401	2406	.	+	0	ID=annotation2137363;Name=Freiburg scar
BBa_K525311	.	non_covalent_binding_site	4054	4059	.	+	0	ID=annotation2137369;Name=AgeI
BBa_K525311	.	non_covalent_binding_site	76	81	.	+	0	ID=annotation2137365;Name=NgoMIV
BBa_K525311	.	ribosome_entry_site	56	59	.	+	0	ID=annotation2137353;Name=RBS
>BBa_K525311
taatacgactcactataggggaattgtgagcggataacaattcctactagagaaagaggagaaatactagagatggccg
gcgctaccaagctcgacaagatgcgccaggagctgaaggcagctgttgacgcaaaggatctcaagaaggctgaggaact
gtaccacaagatctcctacgagcttaagacccgtactgttatcctcgaccgcgtctacggccagtccacccgtgagctg
ctccgctccaccttcaaggcagacgctcaggcactgcgtgattctcttatctacgacatcaccgttgctatgaaggcac
gcgaagctcaggacgcagttaaggctggtaacctcgataaggcaaaggctgcactggaccaggtcaaccagtacgtttc
caaggttactgacgctttcaaggcagagctccagaaggctgcacaggatgctaaggcagcttacgaggcagctctgcca
ccaaaggtcgaatccgtgaccgcagttaacgctaagacccttgagatcaagttcaacaaggcagttgacgctgcaaccg
tcatcgacaacaagggcacttccgatacctctgacgacgttgttaaggctaccgcaatcaccctcaaggctatcgatga
ccaggtccctgtttccactgttaaggcatccctgtccgacgataagaagaccctcaagctggtcgtggacggcgctcag
ttcttcaccaagcgttacgttgttgacatcaagaacgtcaagacccttgatggtaaggacgttccagcatacactacca
ccatcgacaccactgattctgttcgcccatccgtcctctccttctcctacgctgacaacggcctgaccctcaaggttaa
gttctctgagcctctggcatccgttggcaccgtcaagctttacgacggtaccactgaaatctccgtgtccccaaagttc
accgctggcgatgacgagatgaccatcaacctcgcatcttcctccgttccagttaacaaggacctgaccctcaagatct
tcggcgctgtcgattacaacggtaacgttatcaaccctaacccagcagagctgactgttaagaagaccaccgtcgacat
caccaagccagttgttcagtccatcgaagctgtcaacactaagaccgtgaaggttaccttctctgagaagcttctctcc
gcacctaccatcaagatcggcggccagactgcttccgtttccgtcgactctactggtctggtttacaccgcaaccctcg
cttccgcactgtccaagggcgtttacgctgtcgaggtttccgattacaaggaccttgcaggcaactctggtgacgctta
cactaaggttgtccagctcaaggcagataacaccgctccaaagttcgtgtcctcccaggttgttaagatcaacggcgtc
gaacacctggttctcaccttcgacgaggaggttaccactggctccaacatcaccgtcgttcagtcttccgacaagtaca
tcgatgaaaacaacgttctgaaggcagtcggtgctgaccttaagaccacctccgacaacttcaagctctacctgccaac
tgatggcaagtccaagtctgtggcactcaacatctcctccctgcctaagggcacctacaccgttacccttccaaacggt
ctcgtttccgacctggctgacaacccatacgcagagcgtaagcagatcactttcgtccgcggctctgattccctcacca
ccaagcctgctctggacaaggactacgattccaacggcgttaaggcagacaacaacaacgagcttgttttcgctttcac
ccagaacctcgacgcatccgctctgaacctctctaacttcaacatcaacggtctgactgtcaccaaggcagttttcgat
ggcgacaccaagcacatccgtgttacccttgctccaggcgcaaacacttggactggtacccacgtcatcaccatctcca
acatcaagaacacttccggcctcgtgatggacaccgttaccgttaacgaatacatgaaggagaacgtcgctccaacctt
cactgcaaccctgacctccgctgatgttatccgcgttgacttctctgagcctgtcgcaaacgctaccatctcccgtgca
ctctccgctaacaacttcatcgttaaggttgacggcaacgtcgtgactgtttccaacgtttacgaagataacaacgcaa
ccaacctggtccagggttctaagggctacaagaccgtttaccttaagctccagtccccagttaccgacctgtccaagcc
aatcactctctccgctaccgacatcgtcgatgttaaccagaccggcgcaatcaccgacaacaacgttgtcggtaacaac
gtgtctgctactgttgttaacgtcgcaaagaccggcgaagacgccaaaaacataaagaaaggcccggcgccattctatc
cgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttt
tacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaa
cgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcg
cgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttc
gcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaa
aaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctc
ccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctgg
atctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatt
tttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacac
tcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcagga
ttacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgat
ttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttcc
atctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataa
accgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgtt
aatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgcct
tgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccg
cctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaac
atcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacg
gaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagt
tgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggcc
aagaagggcggaaagatcgccgtgaccggttaa