LOCUS       BBa_K525405             3921 bp    DNA     linear       25-Nov-2024
DEFINITION  Fusion Protein of S-Layer SbpA and mCitrine
ACCESSION   BBa_K525405
VERSION     BBa_K525405.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=T7 promoter
     misc_feature    20..44
                     /label=lac operator
     misc_feature    53..64
                     /label=B0034
     RBS             56..59
                     /label=RBS
     misc_feature    73..75
                     /label=ATG
     misc_feature    76..81
                     /label=NgoMIV
     misc_feature    3919..3921
                     /label=TAA
     misc_feature    3913..3918
                     /label=AgeI
     misc_feature    73..3192
                     /label=K525401
     CDS             73..3192
                     /label=SbpA domain
     misc_feature    3193..3198
                     /label=Freiburg scar
     CDS             3199..3921
                     /label=mCitrine (YFP)
     misc_feature    3199..3921
                     /label=J18931
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcctactag agaaagagga
       61 gaaatactag agatggccgg cgctcaggtt aacgactaca acaagatctc cggctacgca
      121 aaggaggctg ttcagtccct cgtcgaccag ggtgttatcc agggcgatac caacggcaac
      181 ttcaacccac tgaacaccgt tactcgcgca caggctgctg agatcttcac caaggctctc
      241 gaactggagg cagacggtga cgtcaacttc tccgatgtta agaagggcgc ttggtactac
      301 aactctatcg cagctgttgt cgcaaacggc atcttcgagg gtgtgtccgc taacgagttc
      361 gcaccaaaca agtcccttac ccgttccgag gctgcaaagg ttctcgttga cgctttcggc
      421 ctggagggct ctgaatccct ctcccagttc gcagacgctt cccaggtcaa gggttgggca
      481 aagtctgctc tggagaccgc agttgctaac ggcatcttca ctggctccga ggaaaacggt
      541 aagcttaacc tcaagcctaa cgcagctatc acccgccagg atttcgcagt tgtcttcgct
      601 cgtaccctgg acctcgcagt tgagaccgtt gacgcttccg tcaaggcaat caacaacact
      661 accgtggagg ttaccttcga tgaagaggtt gacaacgtcg aggctctgaa gttcaagatc
      721 gaaggccttg aggttaagaa cgcagctgtt aagcagacca acaagaaggt cgttgttctc
      781 actaccgagg cacagaccgc tgacaaggaa tacgtcgtga ccctggatgg cgaggagatc
      841 ggtaagttca agggcatcga agcagttgtt ccaaagtcca tcgtcctcaa gactaccaac
      901 acccagggca aggttggtaa ccaggttacc ctgactgctg acgtcggcgt taaggcagct
      961 ggcatcccag ttaccttcaa cgtcgacgca cctaccggct ctcttaacaa ggatgctgtg
     1021 gttgaggttt acaccaacgc agagggcatc gcttcctact cctacactca gtacgcacca
     1081 gacgctgacg atgtcaccgt ttacccaacc ggcgcacctc agctccgcgc tttcggtcca
     1141 gtttactggg gcgtcgacaa catcctgacc atcgaagagg ttactaccgg caacaccctc
     1201 gcaaacggtg ttaagaagac ctacaaggtc actttcaagg acccaaagac cggcgctgca
     1261 ctgaccaaca agaagcttaa cgtgtccttc gttgagaaca ccaacgttgc tttcaacgca
     1321 atctctaagg ctactgtcac caacccttcc tccggcctca ccgttacccc ataccagact
     1381 accaccggcc tccaggaaga gatccaggtt accactgatt ccaacggcca ggcaaccttc
     1441 gtcgtttctg gcaccaacac cgctgttact ccatacgtct tcgtggacgg ttcctcctcc
     1501 gttctcggcg tttctaccgt caccggcacc aacaacgtta ctcaggcaac caccgctaac
     1561 aagaagtggg aggcaaccga actgactgct accgcagctc aggttaagtt cgagggtgca
     1621 cagcttaacc accagatcac cgtcgagcgt gacggcgaag aggaggcagc agctcagtac
     1681 ggctccaagc tcaacggtcg cgaatacaag gttaaggttc tggataagga cggcaagcct
     1741 tacgcaaacg gcctcgtcaa catcggtctg gacgaggtgc ttgatcgtaa cctcaacacc
     1801 aactccaagg ctcagttcgc aaacgttact gacggcaccg ctctgaccct caacccaggc
     1861 acctcccagc agggtaagat caagctgaac tctaagggcg aggcaactgt tgtcctttac
     1921 ggcgctaagg gtgaagttgg caccccagtt gtctggatcg accagaacac ctcccagaac
     1981 aaccagtccg gcgttctcga ggatggtgag cctttcttca aggcaccagt ttccaacttc
     2041 cagtacgaac gcgtcatggg cgctgagttc accgtggagg gcaagacttc taaccagaac
     2101 gttggtaccg acggcatcgc aaactacgct ttcgcactga ccaaccagtc cggcaagacc
     2161 ctcactaacc tgatcggtaa gatgacctac gaaatccgta acacctccgg ccagccaatc
     2221 gaggttaaga cccctatctc ctaccgcgac cagaacaacg tctcttccac tcttttcaag
     2281 atcgaggaat acggctccat caccatctcc gctgatgtta tctctacctc caacgcagct
     2341 ttcaccatcg agactgcacc aaacggtaag gctgcaaccg ttgtcgttac cccatccttc
     2401 gttaccgcta acaacttcga cgcatccgct gacaacaacc tcaacggcgt cgtgactgat
     2461 gcacgtgact tcaacaagca cgttaccgct cctgcactga ccgctacctt cggcaacaac
     2521 aaggaggttg gtactaacta caccggcacc gtccagtact tcaacaagga caagaagacc
     2581 atcactttca acggcaagaa cgcagttaag tacgctggtg aatctggcaa gacctacaag
     2641 tacttcggcc tcggttccac cccaatctcc accgcagatg acttcatcac tcagctccag
     2701 aacaacgctg caaacggctc cgttaccgtc acctacaagg ttgttgacaa cgaggtccag
     2761 ttctacatcg tgtctttcaa caacgatggc gctgcaaacg ctgttaagcc agagcctacc
     2821 gcagttgctc caactgacgg taccgtcgca ttcaccaaca cctccttcaa cgcttccgca
     2881 aaccagacta tcaccgttac cgacgctgat cttaacgcag acgctaccgt tgcagaaact
     2941 accaccgtca aggttacctc cggcactgtt gtctacaccc tcaccgctac cgagactggc
     3001 gtgaacaccg gcgttttcac cgcaaccctg actgctgcac agctcgctgt tctgtctgac
     3061 ggcgtcatca ccgcaaccta cgaggatgtt aagaacacca ctggctcctc cgttacccgc
     3121 accgctaccg caactcttaa caccgtcgtt ggtcaggtta actacaacgc agcagctacc
     3181 accgcatact ccaccggcgt gagcaaaggc gaagaactgt ttaccggcgt ggtgccgatt
     3241 ctggtggaac tggatggtga tgtgaacggc cataaattta gcgtgagcgg cgaaggcgaa
     3301 ggtgatgcga cctatggcaa actgaccctg aaatttattt gcaccacggg taaactgccg
     3361 gttccgtggc cgaccctggt gaccaccttt ggctatggcc tgatgtgctt tgcgcgttat
     3421 ccggatcata tgaaacagca cgatttcttt aaaagcgcca tgccggaagg ctatgtgcag
     3481 gaacgcacca tcttttttaa agatgatggc aactataaaa cccgtgcgga agtgaaattt
     3541 gaaggcgata ccctggtgaa ccgtattgaa ctgaaaggca tcgatttcaa agaagatggc
     3601 aacattctgg gccataaact ggaatataac tacaacagcc ataacgtgta tatcatggcg
     3661 gataaacaga aaaacggcat caaagtgaac tttaaaatcc gccacaacat tgaagatggc
     3721 agcgtgcagc tggccgatca ttatcagcag aacaccccga ttggtgatgg cccggtgctg
     3781 ctgccggata accattatct gagctaccag agcaaactga gcaaagatcc gaacgaaaaa
     3841 cgtgatcaca tggtgctgct ggaatttgtg accgcagccg gtattaccct gggcatggat
     3901 gaactgtaca aaaccggtta a
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