LOCUS BBa_K525411 4854 bp DNA linear 22-Nov-2024 DEFINITION Fusion Protein of S-Layer SbpA and Firefly-Luciferase ACCESSION BBa_K525411 VERSION BBa_K525411.1 FEATURES Location/Qualifiers promoter 1..20 /label=T7 promoter misc_feature 20..44 /label=lac operator misc_feature 53..64 /label=B0034 RBS 56..59 /label=RBS misc_feature 73..3192 /label=K525401 CDS 73..3192 /label=SbpA domain misc_feature 73..75 /label=ATG misc_feature 3193..3198 /label=Freiburg scar CDS 3199..4854 /label=Luciferase misc_feature 3199..4854 /label=K525999 misc_feature 4846..4851 /label=AgeI misc_feature 4852..4854 /label=TAA misc_feature 76..81 /label=NgoMIV ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcctactag agaaagagga 61 gaaatactag agatggccgg cgctcaggtt aacgactaca acaagatctc cggctacgca 121 aaggaggctg ttcagtccct cgtcgaccag ggtgttatcc agggcgatac caacggcaac 181 ttcaacccac tgaacaccgt tactcgcgca caggctgctg agatcttcac caaggctctc 241 gaactggagg cagacggtga cgtcaacttc tccgatgtta agaagggcgc ttggtactac 301 aactctatcg cagctgttgt cgcaaacggc atcttcgagg gtgtgtccgc taacgagttc 361 gcaccaaaca agtcccttac ccgttccgag gctgcaaagg ttctcgttga cgctttcggc 421 ctggagggct ctgaatccct ctcccagttc gcagacgctt cccaggtcaa gggttgggca 481 aagtctgctc tggagaccgc agttgctaac ggcatcttca ctggctccga ggaaaacggt 541 aagcttaacc tcaagcctaa cgcagctatc acccgccagg atttcgcagt tgtcttcgct 601 cgtaccctgg acctcgcagt tgagaccgtt gacgcttccg tcaaggcaat caacaacact 661 accgtggagg ttaccttcga tgaagaggtt gacaacgtcg aggctctgaa gttcaagatc 721 gaaggccttg aggttaagaa cgcagctgtt aagcagacca acaagaaggt cgttgttctc 781 actaccgagg cacagaccgc tgacaaggaa tacgtcgtga ccctggatgg cgaggagatc 841 ggtaagttca agggcatcga agcagttgtt ccaaagtcca tcgtcctcaa gactaccaac 901 acccagggca aggttggtaa ccaggttacc ctgactgctg acgtcggcgt taaggcagct 961 ggcatcccag ttaccttcaa cgtcgacgca cctaccggct ctcttaacaa ggatgctgtg 1021 gttgaggttt acaccaacgc agagggcatc gcttcctact cctacactca gtacgcacca 1081 gacgctgacg atgtcaccgt ttacccaacc ggcgcacctc agctccgcgc tttcggtcca 1141 gtttactggg gcgtcgacaa catcctgacc atcgaagagg ttactaccgg caacaccctc 1201 gcaaacggtg ttaagaagac ctacaaggtc actttcaagg acccaaagac cggcgctgca 1261 ctgaccaaca agaagcttaa cgtgtccttc gttgagaaca ccaacgttgc tttcaacgca 1321 atctctaagg ctactgtcac caacccttcc tccggcctca ccgttacccc ataccagact 1381 accaccggcc tccaggaaga gatccaggtt accactgatt ccaacggcca ggcaaccttc 1441 gtcgtttctg gcaccaacac cgctgttact ccatacgtct tcgtggacgg ttcctcctcc 1501 gttctcggcg tttctaccgt caccggcacc aacaacgtta ctcaggcaac caccgctaac 1561 aagaagtggg aggcaaccga actgactgct accgcagctc aggttaagtt cgagggtgca 1621 cagcttaacc accagatcac cgtcgagcgt gacggcgaag aggaggcagc agctcagtac 1681 ggctccaagc tcaacggtcg cgaatacaag gttaaggttc tggataagga cggcaagcct 1741 tacgcaaacg gcctcgtcaa catcggtctg gacgaggtgc ttgatcgtaa cctcaacacc 1801 aactccaagg ctcagttcgc aaacgttact gacggcaccg ctctgaccct caacccaggc 1861 acctcccagc agggtaagat caagctgaac tctaagggcg aggcaactgt tgtcctttac 1921 ggcgctaagg gtgaagttgg caccccagtt gtctggatcg accagaacac ctcccagaac 1981 aaccagtccg gcgttctcga ggatggtgag cctttcttca aggcaccagt ttccaacttc 2041 cagtacgaac gcgtcatggg cgctgagttc accgtggagg gcaagacttc taaccagaac 2101 gttggtaccg acggcatcgc aaactacgct ttcgcactga ccaaccagtc cggcaagacc 2161 ctcactaacc tgatcggtaa gatgacctac gaaatccgta acacctccgg ccagccaatc 2221 gaggttaaga cccctatctc ctaccgcgac cagaacaacg tctcttccac tcttttcaag 2281 atcgaggaat acggctccat caccatctcc gctgatgtta tctctacctc caacgcagct 2341 ttcaccatcg agactgcacc aaacggtaag gctgcaaccg ttgtcgttac cccatccttc 2401 gttaccgcta acaacttcga cgcatccgct gacaacaacc tcaacggcgt cgtgactgat 2461 gcacgtgact tcaacaagca cgttaccgct cctgcactga ccgctacctt cggcaacaac 2521 aaggaggttg gtactaacta caccggcacc gtccagtact tcaacaagga caagaagacc 2581 atcactttca acggcaagaa cgcagttaag tacgctggtg aatctggcaa gacctacaag 2641 tacttcggcc tcggttccac cccaatctcc accgcagatg acttcatcac tcagctccag 2701 aacaacgctg caaacggctc cgttaccgtc acctacaagg ttgttgacaa cgaggtccag 2761 ttctacatcg tgtctttcaa caacgatggc gctgcaaacg ctgttaagcc agagcctacc 2821 gcagttgctc caactgacgg taccgtcgca ttcaccaaca cctccttcaa cgcttccgca 2881 aaccagacta tcaccgttac cgacgctgat cttaacgcag acgctaccgt tgcagaaact 2941 accaccgtca aggttacctc cggcactgtt gtctacaccc tcaccgctac cgagactggc 3001 gtgaacaccg gcgttttcac cgcaaccctg actgctgcac agctcgctgt tctgtctgac 3061 ggcgtcatca ccgcaaccta cgaggatgtt aagaacacca ctggctcctc cgttacccgc 3121 accgctaccg caactcttaa caccgtcgtt ggtcaggtta actacaacgc agcagctacc 3181 accgcatact ccaccggcga agacgccaaa aacataaaga aaggcccggc gccattctat 3241 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg 3301 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag 3361 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat 3421 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg 3481 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc 3541 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa 3601 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa 3661 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt 3721 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc 3781 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc 3841 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact 3901 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat 3961 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg 4021 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc 4081 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt 4141 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt 4201 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg 4261 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat 4321 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct 4381 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat 4441 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt 4501 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa 4561 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat 4621 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa 4681 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga 4741 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc 4801 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgaccgg ttaa //